Detection of Xanthomonas arboricola pv. pruni by PCR Using Primers Based on DNA Sequences Related to the hrp Genes

So Yeon Park; Young Sun Lee; Young Jin Koh; Jae-Sun Hur; Jae Sung Jung

doi:10.1007/s12275-010-0072-3

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Detection of Xanthomonas arboricola pv. pruni by PCR Using Primers Based on DNA Sequences Related to the hrp Genes: So Yeon Park ¹, Young Sun Lee ¹, Young Jin Koh ², Jae-Sun Hur ³, Jae Sung Jung ¹; Journal of Microbiology 2010;48(5):554-558.
DOI: https://doi.org/10.1007/s12275-010-0072-3
Published online: November 3, 2010

¹Department of Biology, ²Department of Plant medicine, ³Department of Environmental Education, Sunchon National University, Suncheon 540-742, Republic of Korea¹Department of Biology, ²Department of Plant medicine, ³Department of Environmental Education, Sunchon National University, Suncheon 540-742, Republic of Korea

Corresponding author: Jae Sung Jung , Tel: +82-61-750-3616,

Received: 23 February 2010 • Accepted: 31 May 2010

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Abstract

Efficient control of Xanthomonas arboricola pv. pruni, the causal agent of bacterial spot on stone fruit, requires a sensitive and reliable diagnostic tool. A PCR detection method that utilizes primers to target the hrp gene cluster region was developed in this study. The nucleotide sequence of the PCR product amplified with primers specific for the hrp region of the xanthomonads and genomic DNA of X. arboricola pv. pruni was determined, and the sequence was aligned with that of X. campestris pv. campestris, which was obtained from the GenBank database. On the basis of the sequence of the amplified hrp region, a PCR primer set of XapF/R specific to X. arboricola pv. pruni was designed. This primer set yielded a 243-bp product from the genomic DNA of X. aboricola pv. pruni strains, but no products from other 21 strains of Xanthomonas or from two epiphytic bacterial species. Southern blot hybridization with the probe derived from the PCR product with the primer set and X. aboricola pv. pruni DNA confirmed the PCR results. The Xap primer system was successfully applied to detect the pathogen from infected peach fruits. When it was applied in field samples, the primer set was proved as a reliable diagnostic tool for specific detection of X. aboricola pv. pruni from peach orchards.

Keywords: bacterial spot;detection;PCR;X. arboricola pv. pruni

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Detection of Xanthomonas arboricola pv. pruni by PCR Using Primers Based on DNA Sequences Related to the hrp Genes

J. Microbiol. 2010;48(5):554-558. Published online November 3, 2010

DOI: https://doi.org/10.1007/s12275-010-0072-3

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