NOTE] Analysis of Cytoplasmic Membrane Proteome of Streptococcus pneumoniae by Shotgun Proteomic Approach

Chi-Won Choi; Sung-Ho Yun; Sang-Oh Kwon; Sun-Hee Leem; Jong-Soon Choi; Chi-Young Yun; Seung Il Kim

doi:10.1007/s12275-010-0220-9

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NOTE] Analysis of Cytoplasmic Membrane Proteome of Streptococcus pneumoniae by Shotgun Proteomic Approach: Chi-Won Choi ^1,2, Sung-Ho Yun ¹, Sang-Oh Kwon ^1,3, Sun-Hee Leem ⁴, Jong-Soon Choi ^1,3, Chi-Young Yun ², Seung Il Kim ¹; Journal of Microbiology 2010;48(6):872-876.
DOI: https://doi.org/10.1007/s12275-010-0220-9
Published online: January 9, 2011

¹Devision of Life Science, Korea Basic Science Institute, Daejeon 305-806, Republic of Korea, ²Department of Biology, Daejeon University, Daejeon 300-716, Republic of Korea, ³Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, Republic of Korea, ⁴Department of Biology and Biomedical Science, Dong-A University, Busan 604-714, Republic of Korea

Corresponding author: Seung Il Kim , Tel: +82-42-865-3451,

Received: 15 June 2010 • Accepted: 8 September 2010

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Abstract

In this study, cytoplasmic membrane proteins of S. pneumoniae strain R6 (ATCC BBA-255) were effectively separated from cell wall or extracellular proteins by sodium carbonate precipitation (SCP) and ultracentrifugation. Forty seven proteins were analyzed as cytoplasmic membrane proteins from the 260 proteins identified by the shotgun proteomic method using SDS-PAGE/LC/MS-MS. ABC transporters for metabolites such as metals, oligopeptides, phosphate, sugar, and amino acids, and membrane proteins involved in phosphotransferse systems, were identified as the predominant and abundant, cytoplasmic membrane proteins that would be essential for nutrient uptake, antibiotic resistance and virulence mechanisms. Our result supports that gel-based shotgun proteomics combined with sodium carbonate precipitation and ultracentrifugation is an effective method for analysis of cytoplasmic membrane proteins of S. pneumoniae.

Keywords: Streptococcus pneumoniae;membrane proteome;1-DE/LC/MS-MS

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NOTE] Analysis of Cytoplasmic Membrane Proteome of Streptococcus pneumoniae by Shotgun Proteomic Approach

J. Microbiol. 2010;48(6):872-876. Published online January 9, 2011

DOI: https://doi.org/10.1007/s12275-010-0220-9

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