Abstract
Bacillus amyloliquefaciens LBM 5006 produces antagonistic activity against pathogenic bacteria and phytopathogenic fungi, including Aspergillus spp., Fusarium spp., and Bipolaris sorokiniana. PCR analysis revealed the presence of ituD, but not sfp genes, coding for iturin and surfactin, respectively. The antimicrobial substance produced by this strain was isolated by ammonium sulfate precipitation, gel filtration chromatography and 1-butanol extraction. The ultraviolet spectrum was typical of a polypeptide and the infrared spectrum indicates the presence of peptide bonds and acyl group(s). The antimicrobial substance was resistant to proteolytic enzymes and heat treatment, and was reactive with ninhydrin. Mass spectroscopy analysis indicated that B. amyloliquefaciens LBM 5006 produces two antimicrobial peptides, with main peaks at m/z 1,058 Da and 1,464 Da, corresponding to iturin-like and fengycin-like peptides, respectively. B. amyloliquefaciens LBM 5006 showed significant activity against phytopatogenic fungi, showing potential for use as a biocontrol agent or production of antifungal preparations.
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- Study on the Inhibitory Effects of Three Endophytic Bacillus Strains on Aspergillus flavus in Maize
Siyu Ma, Min Li, Siqi Zhang, Yin Yang, Fengsha Zhu, Xingyu Li, Shahzad Munir, Pengfei He, Pengbo He, Yixin Wu, Yueqiu He, Ping Tang
Metabolites.2025; 15(4): 268. CrossRef - Culture Optimization of Endophytic and Rhizosphere Bacteria and Exploration of Antifungal Substances
Y. Sheng, X. Li, H. Yang, M. Chu, X. Niu, N. Wang, H. Bao, F. Zhan, R. Yang, K. Lou, Y. Shi, G. Zhang
Applied Biochemistry and Microbiology.2025;[Epub] CrossRef - Enhancing Phosphorus Uptake and Mitigating Lead Stress in Maize Using the Rhizospheric Fungus Talaromyces purpureogenus PH7
Iqbal Hussain, Muhammad Irshad, Anwar Hussain, Muhammad Qadir, Asif Mehmood, Muneeb urrehman, Naeem Khan
CLEAN – Soil, Air, Water.2025;[Epub] CrossRef - Phosphate solubilizing Aspergillus Niger PH1 ameliorates growth and alleviates lead stress in maize through improved photosynthetic and antioxidant response
Iqbal Hussain, Muhammad Irshad, Anwar Hussain, Muhammad Qadir, Asif Mehmood, Muneebur Rahman, Abdulwahed Fahad Alrefaei, Mikhlid H. Almutairi, Sajid Ali, Muhammad Hamayun
BMC Plant Biology.2024;[Epub] CrossRef - Genome analysis of Pseudomonas strain 4B with broad antagonistic activity against toxigenic fungi
Flávio Fonseca Veras, Paolo Stincone, Juliane Elisa Welke, Ana Carolina Ritter, Franciele Maboni Siqueira, Ana Paula Muterle Varela, Fabiana Quoos Mayer, Adriano Brandelli
Brazilian Journal of Microbiology.2024; 55(1): 269. CrossRef - Genomic Insights into the Bactericidal and Fungicidal Potential of Bacillus mycoides b12.3 Isolated in the Soil of Olkhon Island in Lake Baikal, Russia
Maria N. Romanenko, Anton E. Shikov, Iuliia A. Savina, Fedor M. Shmatov, Anton A. Nizhnikov, Kirill S. Antonets
Microorganisms.2024; 12(12): 2450. CrossRef - Antagonistic effects of Bacillus species against bacterial multi-drug resistant (MDR) food-borne pathogens and aflatoxigenic fungi
Temilade Ozabor, Deborah Falomo, Elizabeth Taiwo, Oyindamola Alabi, Precious Adediran, Femi Ayoade, Ilesanmi Fadahunsi, Janet Olaitan
UMYU Journal of Microbiology Research (UJMR).2024; : 89. CrossRef - Evaluation of the mechanism of action of Bacillus spp. to manage Meloidogyne incognita with split root assay, RT-qPCR and qPCR
Kaitlin M. Gattoni, Sang Wook Park, Kathy S. Lawrence
Frontiers in Plant Science.2023;[Epub] CrossRef - Microbial lipopeptides: Properties, mechanics and engineering for novel lipopeptides
Smitha Mony Sreedharan, Niharika Rishi, Rajni Singh
Microbiological Research.2023; 271: 127363. CrossRef - Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans
Elizabeth Teixeira de Almeida Ramos, Carlos Henrique Salvino Gadelha Meneses, Marcia Soares Vidal, José Ivo Baldani
Annals of Applied Biology.2022; 180(1): 163. CrossRef - Isolation and characterization of a new strain of Bacillus amyloliquefaciens and its effect on strawberry preservation
Tingting Ying, Peijun Wu, Linlin Gao, Congcong Wang, Tonghe Zhang, Sisi Liu, Ruqiang Huang
LWT.2022; 165: 113712. CrossRef - Bacillus subtilis strain F62 against Fusarium oxysporum and promoting plant growth in the grapevine rootstock SO4
ALESSANDRA RUSSI, MARCUS ANDRÉ K. ALMANÇA, JOSÉLI SCHWAMBACH
Anais da Academia Brasileira de Ciências.2022;[Epub] CrossRef - Influence of Heat Stress on Poultry Growth Performance, Intestinal Inflammation, and Immune Function and Potential Mitigation by Probiotics
Rafiq Ahmad, Yu-Hsiang Yu, Felix Shih-Hsiang Hsiao, Chin-Hui Su, Hsiu-Chou Liu, Isabel Tobin, Guolong Zhang, Yeong-Hsiang Cheng
Animals.2022; 12(17): 2297. CrossRef - In Vitro and In Vivo Anti-Clostridioides difficile Effect of a Probiotic Bacillus amyloliquefaciens Strain
Md Imtiazul Islam, Hoonhee Seo, Asma Redwan, Sukyung Kim, Saebim Lee, Mashuk Siddiquee, Ho-Yeon Song
Journal of Microbiology and Biotechnology.2022; 32(1): 46. CrossRef - A review on characterization, applications and structure-activity relationships of Bacillus species-produced bacteriocins
Shradha Basi-Chipalu, Pallavi Sthapit, Saphala Dhital
Drug Discoveries & Therapeutics.2022; 16(2): 55. CrossRef - The Effect of Surfactants of Microbial Origin on Phytopathogenic Microorganisms
T.P. Pirog, D.V. Piatetska, H.А. Yarova, G.O. Iutynska
Mikrobiolohichnyi Zhurnal.2021; 83(6): 75. CrossRef - Bacterial and yeast microbial diversity of the ripened traditional middle east surk cheese
Yusuf Esen, Bülent Çetin
International Dairy Journal.2021; 117: 105004. CrossRef - Biocontrol of black foot disease on grapevine rootstocks using Bacillus subtilis strain F62
Alessandra Russi, Marcus André Kurtz Almança, Daniel Santos Grohs, Joséli Schwambach
Tropical Plant Pathology.2020; 45(2): 103. CrossRef - Cell-Free Supernatants of Plant Growth-Promoting Bacteria: A Review of Their Use as Biostimulant and Microbial Biocontrol Agents in Sustainable Agriculture
Marika Pellegrini, Giancarlo Pagnani, Matteo Bernardi, Alessandro Mattedi, Daniela M. Spera, Maddalena Del Gallo
Sustainability.2020; 12(23): 9917. CrossRef - Prebiotic, Probiotic, Antimicrobial, and Functional Food Applications of Bacillus amyloliquefaciens
Kalekristos WoldemariamYohannes, Zhen Wan, Qinglin Yu, Hongyan Li, Xuetuan Wei, Yingli Liu, Jing Wang, Baoguo Sun
Journal of Agricultural and Food Chemistry.2020; 68(50): 14709. CrossRef - The response strategies of Colletotrichum gloeosporioides s.s. due to the stress caused by biological control agent Bacillus amyloliquefaciens deciphered by transcriptome analyses
Qing-Hai Wang, Yan-Ping Ji, Yong-Yun Qu, Yu-Kun Qi, De-Wei Li, Zhen-Yu Liu, Xiao-Qin Wu
Biological Control.2020; 150: 104372. CrossRef - Biocontrol of Fusarium wilt disease in strawberries using bioorganic fertilizer fortified with Bacillus licheniformis X-1 and Bacillus methylotrophicus Z-1
Yan Chen, Yongping Xu, Tong Zhou, Mahinur S. Akkaya, Lili Wang, Shuying Li, Xiaoyu Li
3 Biotech.2020;[Epub] CrossRef -
Antimicrobial
Bacillus velezensis
HC6: production of three kinds of lipopeptides and biocontrol potential in maize
Y. Liu, K. Teng, T. Wang, E. Dong, M. Zhang, Y. Tao, J. Zhong
Journal of Applied Microbiology.2020; 128(1): 242. CrossRef - A novel antimicrobial protein of the endophytic Bacillus amyloliquefaciens and its control effect against Fusarium chlamydosporum
Han-mingyue Zhu, Yuan-zhi Pan
BioControl.2019; 64(6): 737. CrossRef - Antagonist effects of strains of Bacillus spp. against Rhizoctonia solani for their protection against several plant diseases: Alternatives to chemical pesticides
Aqleem Abbas, Shahid Ullah Khan, Wasim Ullah Khan, Tawfik A. Saleh, Muhammad Hafeez Ullah Khan, Sana Ullah, Ahmad Ali, Muhammad Ikram
Comptes Rendus. Biologies.2019; 342(5-6): 124. CrossRef - Phân lập và định danh vi khuẩn nội sinh ở cây diếp cá (Houttuynia cordata Thunb, Saururaceae) tại tỉnh Kiên Giang có hoạt tính kháng khuẩn với vi khuẩn Staphylococcus aureus từ mụn nhọt ở người
Huỳnh Văn Trương, Nguyễn Hữu Hiệp, Lý Tú Hương
Can Tho University Journal of Science.2019; 55(Công ng: 166. CrossRef - Identification of the biocontrol strain LB-2 and determination of its antifungal effects on plant pathogenic fungi
Caiyun Liu, Z. Chang
Journal of Plant Pathology.2018; 100(1): 25. CrossRef - Identification and characterization of a novel antimicrobial peptide compound produced by Bacillus megaterium strain isolated from oral microflora
Abdullah S.A. Al-Thubiani, Yahia A. Maher, Adel Fathi, Mohammed A.S. Abourehab, Mohammed Alarjah, Mohd S.A. Khan, Saleh B. Al- Ghamdi
Saudi Pharmaceutical Journal.2018; 26(8): 1089. CrossRef - Antimicrobial discovery from natural and unusual sources
Salwa Mansur Ali, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Journal of Pharmacy and Pharmacology.2018; 70(10): 1287. CrossRef -
Bacillus amyloliquefaciens
induces production of a novel blennolide K in coculture of Setophoma terrestris
D. Arora, G. Chashoo, V. Singamaneni, N. Sharma, P. Gupta, S. Jaglan
Journal of Applied Microbiology.2018; 124(3): 730. CrossRef - Identification of a small antimycotic peptide produced by Bacillus amyloliquefaciens 6256
Qing Xia Zhang, Ying Zhang, Ling Ling He, Zhao Lin Ji, Yun Hui Tong
Pesticide Biochemistry and Physiology.2018; 150: 78. CrossRef - Biological control of plant pathogens by Bacillus species
Djordje Fira, Ivica Dimkić, Tanja Berić, Jelena Lozo, Slaviša Stanković
Journal of Biotechnology.2018; 285: 44. CrossRef - Molecular characterization of bmyC gene of the mosquito pupicidal bacteria, Bacillus amyloliquefaciens (VCRC B483) and in silico analysis of bacillomycin D synthetase C protein
Mathivanan Ashokkumar, Geetha Irudayaraj, Nandakumar Yellapu, Arulsamy Mary Manonmani
World Journal of Microbiology and Biotechnology.2018;[Epub] CrossRef - Isolation and identification of antifungal peptides from Bacillus amyloliquefaciens W10
Qing-Xia Zhang, Ying Zhang, Hai-Huan Shan, Yun-Hui Tong, Xi-Jun Chen, Feng-Quan Liu
Environmental Science and Pollution Research.2017; 24(32): 25000. CrossRef - Biological characteristics ofBacillus amyloliquefaciensAK-0 and suppression of ginseng root rot caused byCylindrocarpon destructans
Y.S. Kim, K. Balaraju, Y.H. Jeon
Journal of Applied Microbiology.2017; 122(1): 166. CrossRef - Bacillusspecies as versatile weapons for plant pathogens: a review
Jamil Shafi, Hui Tian, Mingshan Ji
Biotechnology & Biotechnological Equipment.2017; 31(3): 446. CrossRef - Antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247
Atsushi Kurata, Yuto Yamaura, Takumi Tanaka, Chiaki Kato, Kaoru Nakasone, Noriaki Kishimoto
World Journal of Microbiology and Biotechnology.2017;[Epub] CrossRef - Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments
Yulin Chen, Shiliang A. Liu, Haijin Mou, Yunxiao Ma, Meng Li, Xiaoke Hu
Frontiers in Microbiology.2017;[Epub] CrossRef - Identification for the First Time of Cyclo(d-Pro-l-Leu) Produced by Bacillus amyloliquefaciens Y1 as a Nematocide for Control of Meloidogyne incognita
Qaiser Jamal, Jeong-Yong Cho, Jae-Hak Moon, Shahzad Munir, Muhammad Anees, Kil Yong Kim
Molecules.2017; 22(11): 1839. CrossRef - Partial purification and characterization of bacteriocin produced byEnterococcus faecalisDU10 and its probiotic attributes
Venkatesh Perumal, Ayyanna Repally, Ankaiah Dasari, Arul Venkatesan
Preparative Biochemistry & Biotechnology.2016; 46(7): 686. CrossRef - Isolation and anti- Verticillium dahliae activity from Bacillus axarquiensis TUBP1 protein
Hong Zeng, Rong Chen, Xiaoxia Luo, Jun Tian
Process Biochemistry.2016; 51(10): 1691. CrossRef - Towards bacterial lipopeptide products for specific applications — a review of appropriate downstream processing schemes
Vivek Rangarajan, Kim G. Clarke
Process Biochemistry.2016; 51(12): 2176. CrossRef - Complete genome sequence of Bacillus amyloliquefaciens B15 isolated from grape skin, a strain of strong inhibitory activity against fungi
Yinzhuo Yan, Shiyu Liu, Deliang Wang, Jie Xue, Danyang Guo, Xulei Song, Fengjie Zhang, Shihai Huang, Chunguang Luan
Journal of Biotechnology.2016; 228: 28. CrossRef - Inhibition of mycotoxin-producing fungi by Bacillus strains isolated from fish intestines
Flávio Fonseca Veras, Ana Paula Folmer Correa, Juliane Elisa Welke, Adriano Brandelli
International Journal of Food Microbiology.2016; 238: 23. CrossRef - Postharvest decay control of a “long-storage” tomato landrace using different preharvest treatments
R. Carrieri, M. Parisi, F. Di Dato, P. Tarantino, F. De Vita, R. Perreca, R. Riccardi, E. Lahoz
Acta Horticulturae.2016; (1144): 423. CrossRef - Antimicrobial peptides of the genus Bacillus: a new era for antibiotics
Chandra Datta Sumi, Byung Wook Yang, In-Cheol Yeo, Young Tae Hahm
Canadian Journal of Microbiology.2015; 61(2): 93. CrossRef - Characterization of Bacitracin Zinc by Matrix-Assisted Laser Desorption Ionization – Tandem Time-of-Flight Mass Spectrometry
Wen-jie Zhao, Jun-qin Qiao, Qun Song, Hong-zhen Lian
Instrumentation Science & Technology.2015; 43(4): 453. CrossRef - Antagonistic Activities of Novel Peptides from Bacillus amyloliquefaciens PT14 against Fusarium solani and Fusarium oxysporum
Young Gwon Kim, Hee Kyoung Kang, Kee-Deok Kwon, Chang Ho Seo, Hyang Burm Lee, Yoonkyung Park
Journal of Agricultural and Food Chemistry.2015; 63(48): 10380. CrossRef - Structural characterization and temperature-dependent production of C17-fengycin B derived from Bacillus amyloliquefaciens subsp. plantarum BC32-1
Jiyoung Nam, Min Young Jung, Pyoung Il Kim, Hyang Burm Lee, Si Wouk Kim, Chul Won Lee
Biotechnology and Bioprocess Engineering.2015; 20(4): 708. CrossRef - Bacteriocinogenic potential of a probiotic strain Bacillus coagulans [BDU3] from Ngari
Kaja Abdhul, Mohan Ganesh, Santhanam Shanmughapriya, Shanmugam Vanithamani, Murugesan Kanagavel, Kumarasamy Anbarasu, Kalimuthusamy Natarajaseenivasan
International Journal of Biological Macromolecules.2015; 79: 800. CrossRef - Bacillus amyloliquefaciens Q‐426 as a potential biocontrol agent against Fusarium oxysporum f. sp. spinaciae
Pengchao Zhao, Chunshan Quan, Yingguo Wang, Jianhua Wang, Shengdi Fan
Journal of Basic Microbiology.2014; 54(5): 448. CrossRef - Inhibition of conidiophore development in Aspergillus fumigatus by an Escherichia coli DH5α strain, a promising antifungal candidate against aspergillosis
M. Balhara, S. Ruhil, M. Kumar, S. Dhankhar, A.K. Chhillar
Journal de Mycologie Médicale.2014; 24(1): 1. CrossRef -
Bacillus amyloliquefaciens
ssp. plantarum
strains as potential protective starter cultures for the production of Bikalga
, an alkaline fermented food
C.S. Compaoré, D.S. Nielsen, H. Sawadogo-Lingani, T.S. Berner, K.F. Nielsen, D.B. Adimpong, B. Diawara, G.A. Ouédraogo, M. Jakobsen, L. Thorsen
Journal of Applied Microbiology.2013; 115(1): 133. CrossRef - Co-production of surfactin and a novel bacteriocin by Bacillus subtilis subsp. subtilis H4 isolated from Bikalga, an African alkaline Hibiscus sabdariffa seed fermented condiment
Clarisse S. Compaoré, Dennis S. Nielsen, Labia I.I. Ouoba, Torben S. Berner, Kristian F. Nielsen, Hagrétou Sawadogo-Lingani, Bréhima Diawara, Georges A. Ouédraogo, Mogens Jakobsen, Line Thorsen
International Journal of Food Microbiology.2013; 162(3): 297. CrossRef - Isolation and characterization of an antimicrobial substance from Bacillus subtilis BY08 antagonistic to Bacillus cereus and Listeria monocytogenes
Wei-Jie Wu, Seung-Moon Park, Byung-Yong Ahn
Food Science and Biotechnology.2013; 22(2): 433. CrossRef - Control of Listeria monocytogenes on sliced bologna sausage using a novel bacteriocin, amysin, produced by Bacillus amyloliquefaciens isolated from Thai shrimp paste (Kapi)
Siriporn Kaewklom, Saovaluk Lumlert, Wassawan Kraikul, Ratchaneewan Aunpad
Food Control.2013; 32(2): 552. CrossRef - Antimicrobial factor from Bacillus amyloliquefaciens inhibits Paenibacillus larvae, the causative agent of American foulbrood
Lisianne Brittes Benitez, Renata Voltolini Velho, Amanda de Souza da Motta, Jéferson Segalin, Adriano Brandelli
Archives of Microbiology.2012; 194(3): 177. CrossRef - Isolation of Bacillus amyloliquefaciens Strains with Antifungal Activities from Meju
Hwang-A Lee, Jeong-Hwan Kim
Preventive Nutrition and Food Science.2012; 17(1): 64. CrossRef
MSK
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