Abstract
			
			L-Methioninase was purified to electrophoretic homogeneity from cultures of Aspergillus flavipes using anionexchange and gel filtration chromatography by 12.1 fold compared to the crude enzyme preparation. The purified enzyme had a molecular mass of 47 kDa under denaturing conditions and an isoelectric point
of 5.8 with no structural glycosyl residues. The enzyme had optimum activity at pH 7.8 and pH stability from 6.8-8.0 at 35°C. The enzyme appeared to be catalytically stable below 40°C. The enzyme activity was strongly inhibited by DL-propargylglycine, hydroxylamine, PMSF, 2-mercaptoethanol, Hg2+, Cu2+, and
Fe2+, with slight inhibition by Triton X-100. A. flavipes L-methioninase has a higher catalytic affinity towards L-methionine (Km, 6.5 mM and Kcat, 14.1 S-1) followed by a relative demethiolating activity to L-homocysteine (Km, 12 mM and Kcat, 9.3 S-1). The enzyme has two absorption maxima at 280 and 420 nm, typical of other PLP-enzymes. Apo-L-methioninase has the ability to reconstitute its structural catalytic
state completely upon addition of 0.15 mM PLP. L-Methioninase has neither an appreciable effect on liver function, platelet aggregation, nor hemolysis of human blood. The purified L-methioninase from solid cultures of A. flavipes displayed unique biochemical and catalytic properties over the currently applied Pseudomonad
enzyme.			
						
						
					 
		
		
		 
		
			Citations
			Citations to this article as recorded by  

- Purification, characterization, and anti-cancer activity of methionine gamma-lyase from a native strain of Pseudomonas mosselii for human cancer treatment
 Matin Nasrian, Mohsen Mobini-Dehkordi, Pegah Khosravian
 BMC Biotechnology.2025;[Epub]     CrossRef
- Biochemical Characterization of Thermostable Acrylamide Amidohydrolase from Aspergillus fumigatus with Potential Activity for Acrylamide Degradation in Various Food Products
 Ashraf S. A. El-Sayed, Hala N. Elghamry, Marwa A. Yassin
 Current Microbiology.2024;[Epub]     CrossRef
- Bioprocessing of Epothilone B from Aspergillus fumigatus under solid state fermentation: Antiproliferative activity, tubulin polymerization and cell cycle analysis
 Ashraf S. A. El-Sayed, Ahmed Shindia, Hala Ammar, Mohamed G. Seadawy, Samar A. Khashana
 BMC Microbiology.2024;[Epub]     CrossRef
- Enzymatic properties of ornithine decarboxylase from Clostridium aceticum DSM1496
 Qian Tan, Linbo Gou, Tai‐Ping Fan, Yujie Cai
 Biotechnology and Applied Biochemistry.2024; 71(3): 525.     CrossRef
- Potential fungicidal and antiaflatoxigenic effects of cinnamon essential oils on Aspergillus flavus inhabiting the stored wheat grains
 Manar M. Abdel Gwad, Ashraf S. A. El-Sayed, Gamal M. Abdel-Fattah, Mohamed Abdelmoteleb, Ghada G. Abdel-Fattah
 BMC Plant Biology.2024;[Epub]     CrossRef
- Aspergillus flavipes L-methionine γ-lyase-β-cyclodextrin conjugates with improved stability, catalytic efficiency and anticancer activity
 Ashraf S. A. El-Sayed, Ahmed Shindia, Esraa Emam, Mai Labib, Eman Nour El-Deen, Mohamed G. Seadawy, Marwa A. Yassin
 Scientific Reports.2024;[Epub]     CrossRef
- Biochemical, molecular and anti-tumor characterization of L-methionine gamma lyase produced by local Pseudomonas sp. in Egypt
 Azza A. Abou Zeid, Asmaa H. Mohamed, Ashraf S.A. El-Sayed, Ashraf M. EL-Shawadfy
 Saudi Journal of Biological Sciences.2023; 30(7): 103682.     CrossRef
- Isolation and characterization of Trichoderma harzianum L-methioninase with promising a powerful anticancer
 Mada F. Ashkan, Sadia A. Younis, Nahla T. Elazab
 Saudi Journal of Biological Sciences.2023; 30(12): 103870.     CrossRef
- Aspergillus Niger thermostable Cytosine deaminase-dextran conjugates with enhanced structure stability, proteolytic resistance, and Antiproliferative activity
 Ashraf S. A. El-Sayed, Amgad M. Rady, Hossam Taha Mohamed, Nabila Zein, Marwa A. Yassin, Nabil Z. Mohamed, Abdallah Hassan, Mahmoud M. Amer, Reyad El-Sharakawy, Aya Ali El-Sharkawy, Nesma El-Sayed, Mostafa G. Ali
 BMC Microbiology.2023;[Epub]     CrossRef
- Purification, Characterization and anticancer activity of L-methionine γ-lyase from thermo-tolerant Aspergillus fumigatus
 Mahmoud H. Hendy, Amr H. Hashem, Waleed B. Suleiman, Mahmoud H. Sultan, Mohamed Abdelraof
 Microbial Cell Factories.2023;[Epub]     CrossRef
- Bioprospecting of a Thermostable L-Methioninase from Alcaligenes aquatilis BJ-1 in Agro-Industrial Waste
 Bhumi Javia, Megha Gadhvi, Suhas Vyas, Pravin Dudhagara, Douglas J. H. Shyu, Yih-Yuan Chen, Dushyant Dudhagara
 Microbiology Research.2023; 14(3): 959.     CrossRef
- Recombinant production, purification, and biochemical characterization of a novel L-lactate dehydrogenase from Bacillus cereus NRC1 and inhibition study of mangiferin
 Sayed S. Esa, Ahmed F. El-Sayed, Mohamed I. El-Khonezy, Shubing Zhang
 Frontiers in Bioengineering and Biotechnology.2023;[Epub]     CrossRef
- Thermostable Chitosan-L-Asparaginase conjugate from Aspergillus fumigatus is a novel structurally stable composite for abolishing acrylamide formation in French fried potatoes
 Marwa A. Yassin, Ahmed Shindia, Mai Labib, Mohamed Soud, Ashraf S.A. El-Sayed
 LWT.2022; 162: 113494.     CrossRef
- Enhancement of production of l-methioninase after optimizing culture condition of Pseudomonas stutzeri using artificial neural network
 Bhawana Kharayat, Priyanka Singh, Shailendra Singh Shera, Rathindra Mohan Banik
 Vegetos.2022; 35(2): 453.     CrossRef
- Screening, characterization and anti-cancer application of purified intracellular MGL
 Bhupender Sharma, Sunita Devi, Rakesh Kumar, Shamsher Singh Kanwar
 International Journal of Biological Macromolecules.2022; 217: 96.     CrossRef
- Application of Principal Component Analysis (PCA) to the Evaluation and Screening of Multiactivity Fungi
 Zonglin Yang, Yaqi Shi, Pinglin Li, Kanghong Pan, Guoqiang Li, Xianguo Li, Shuo Yao, Dahai Zhang
 Journal of Ocean University of China.2022; 21(3): 763.     CrossRef
- Biochemical Properties of Tyrosinase from Aspergillus terreus and Penicillium copticola; Undecanoic Acid from Aspergillus flavus, an Endophyte of Moringa oleifera, Is a Novel Potent Tyrosinase Inhibitor
 Hanaa Salah Maamoun, Gamal H. Rabie, Ibrahim Shaker, Bothaina A. Alaidaroos, Ashraf S. A. El-Sayed
 Molecules.2021; 26(5): 1309.     CrossRef
- Utilization of orange pulp and corn steep liquor for L-methioninase production by Wickerhamomyces subpelliculosus
 Amany A. Hassabo, Elsayed E. Mostafa, Moataza M. Saad, Mohsen H. Selim
 Egyptian Pharmaceutical Journal.2021; 20(1): 8.     CrossRef
- Computational Elucidation of Phylogenetic, Functional and Structural Features of Methioninase from Pseudomonas, Escherichia, Clostridium and Citrobacter Strains
 Cambyz Irajie, Milad Mohkam , Bahareh  Vakili, Navid  Nezafat
 Recent Patents on Biotechnology.2021; 15(4): 286.     CrossRef
- Exploiting the Biosynthetic Potency of Taxol from Fungal Endophytes of Conifers Plants; Genome Mining and Metabolic Manipulation
 Ashraf S.A. El-Sayed, Manal T. El-Sayed, Amgad M. Rady, Nabila Zein, Gamal Enan, Ahmed Shindia, Sara El-Hefnawy, Mahmoud Sitohy, Basel Sitohy
 Molecules.2020; 25(13): 3000.     CrossRef
- Purification, characterization and anticancer evaluation of l-methioninase from Trichoderma harzianum
 Nisha Salim, A. Santhiagu, K. Joji
 3 Biotech.2020;[Epub]     CrossRef
- Aspergillus flavipes is a novel efficient biocontrol agent of Phytophthora parasitica
 Ashraf S.A. El-Sayed, Gul Shad Ali
 Biological Control.2020; 140: 104072.     CrossRef
- Biocatalytic Cascade Reaction for the Asymmetric Synthesis of L‐ and D‐Homoalanine
 Marcus V. de M. Silva, Ingrid C. R. Costa, Rodrigo O. M. A. de Souza, Uwe T. Bornscheuer
 ChemCatChem.2019; 11(1): 407.     CrossRef
- Aspergillus nidulans thermostable arginine deiminase-Dextran conjugates with enhanced molecular stability, proteolytic resistance, pharmacokinetic properties and anticancer activity
 Ashraf S.A. El-Sayed, Ahmed A. Shindia, Azza A. Abou Zeid, Amany M. Yassin, Mahmoud Z. Sitohy, Basel Sitohy
 Enzyme and Microbial Technology.2019; 131: 109432.     CrossRef
- Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity, permanence and application as efficient therapeutic formulations
 Suganya Kannan, Murugan Marudhamuthu
 International Journal of Biological Macromolecules.2019; 141: 218.     CrossRef
- Process modeling and optimization of high yielding L-methioninase from a newly isolated Trichoderma harzianum using response surface methodology and artificial neural network coupled genetic algorithm
 Nisha Salim, A. Santhiagu, K. Joji
 Biocatalysis and Agricultural Biotechnology.2019; 17: 299.     CrossRef
- Purification and Characterization of Ornithine Decarboxylase from Aspergillus terreus; Kinetics of Inhibition by Various Inhibitors
 Ashraf El-Sayed, Nelly George, Marwa Yassin, Bothaina Alaidaroos, Ahmed Bolbol, Marwa Mohamed, Amgad Rady, Safa Aziz, Rawia Zayed, Mahmoud Sitohy
 Molecules.2019; 24(15): 2756.     CrossRef
- Sterol inhibitor “Fluconazole” enhance the Taxol yield and molecular expression of its encoding genes cluster from Aspergillus flavipes
 Ashraf S.A. El-Sayed, Dalia M.I. Ali, Marwa A. Yassin, Rawia A. Zayed, Gul Shad Ali
 Process Biochemistry.2019; 76: 55.     CrossRef
- Amino Acid Degrading Enzymes and their Application in Cancer Therapy
 Vadim S. Pokrovsky, Olga E. Chepikova, Denis Zh. Davydov, Andrey A. Zamyatnin Jr, Alexander N. Lukashev, Elena V. Lukasheva
 Current Medicinal Chemistry.2019; 26(3): 446.     CrossRef
- Biochemical characterization of peptidylarginine deiminase-like orthologs from thermotolerant Emericella dentata and Aspergillus nidulans
 Ashraf S.A. El-Sayed, Ahmed A. Shindia, Azza A. AbouZaid, Amany M. Yassin, Gul Shad Ali, Mahmoud Z. Sitohy
 Enzyme and Microbial Technology.2019; 124: 41.     CrossRef
- Detoxification and Bioremediation of Sulfa Drugs and Synthetic Dyes by Streptomyces mutabilis A17 Laccase Produced in Solid State Fermentation
 Fifi M Reda, Rasha M El-Mekkawy, Noha S Hassan
 Journal of Pure and Applied Microbiology.2019; 13(1): 85.     CrossRef
- Statistically optimized production of extracellular l-methionine γ-lyase by Streptomyces Sp. DMMMH60 and evaluation of purified enzyme in sub-culturing cell lines
 Mohamed Abdelraof, Mohsen Helmy Selim, Mostafa M. Abo Elsoud, Mamdouh Moawad Ali
 Biocatalysis and Agricultural Biotechnology.2019; 18: 101074.     CrossRef
- Optimization of clinical uricase production by Bacillus cereus under submerged fermentation, its purification and structure characterization
 S.M. Khade, S.K. Srivastava, Krishan Kumar, Kedar Sharma, Arun Goyal, A.D. Tripathi
 Process Biochemistry.2018; 75: 49.     CrossRef
- An extensive review on L-methioninase and its potential applications
 Kannan Suganya, Krishnamoorthy Govindan, Palanichamy Prabha, Marudhamuthu Murugan
 Biocatalysis and Agricultural Biotechnology.2017; 12: 104.     CrossRef
- Optimized production and isolation of antibacterial agent from marine Aspergillus flavipes against Vibrio harveyi
 Lei Guo, Cong Wang
 3 Biotech.2017;[Epub]     CrossRef
- Molecular and Spectroscopic Characterization of Aspergillus flavipes and Pseudomonas putida L-Methionine γ-Lyase in Vitro
 Ashraf S. A. El-Sayed, Laura E. Ruff, Salah E. Abdel Ghany, Gul Shad Ali, Sadik Esener
 Applied Biochemistry and Biotechnology.2017; 181(4): 1513.     CrossRef
- Aspergillus flavipes methionine γ-lyase-dextran conjugates with enhanced structural, proteolytic stability and anticancer efficiency
 Ashraf S.A. El-Sayed, Abdalla E.A. Hassan, Ahmed A. Shindia, Shaimaa G. Mohamed, Mahmoud Z. Sitohy
 Journal of Molecular Catalysis B: Enzymatic.2016; 133: S15.     CrossRef
- Biochemical and biophysical characterization of a peroxidase isolated fromEuphorbia tirucalliwith antifungal activity
 Ankita Shukla, Ravi Kumar Gundampati, Medicherla V. Jagannadham
 Biocatalysis and Biotransformation.2016; 34(5): 236.     CrossRef
- Purification, Characterization of L-Methioninase fromCandida tropicalis, and Its Application as an Anticancer
 Mohsen Helmy Selim, El-Zahraa Karm Eldin, Moataza Mahmoud Saad, El-Sayed Eliwa Mostafa, Yosrea Hassan Shetia, Amany Ahmed Hassabo Anise
 Biotechnology Research International.2015; 2015: 1.     CrossRef
- Coimmobilization of l‐methioninase and glutamate dehydrogenase: Novel approach for l‐homoalanine synthesis
 Ashraf S.A. El‐Sayed, Marwa A. Yassin, Hend Ibrahim
 Biotechnology and Applied Biochemistry.2015; 62(4): 514.     CrossRef
- Purification, immobilization, and biochemical characterization ofl‐arginine deiminase from thermophilicAspergillus fumigatusKJ434941: Anticancer activityin vitro
 Ashraf S. A. El‐Sayed, Mohamed N. Hassan, Hend M. S. Nada
 Biotechnology Progress.2015; 31(2): 396.     CrossRef
- Characterization of Glutathione-Homocystine Transhydrogenase as a Novel Isoform of Glutathione S-Transferase from Aspergillus flavipes
                  
 Ashraf S. A. El-Sayed, Abdalla E. Hassan, Marwa A. Yassin, Asmaa M. F. Hassan
 Pharmaceutical Chemistry Journal.2015; 49(6): 373.     CrossRef
- Screening, morphological and molecular characterization of fungi producing cystathionine γ-lyase
 Ashraf El-Sayed, Salwa Khalaf, Gamal Abdel-Hamid, Mohamed El-Batrik
 Acta Biologica Hungarica.2015; 66(1): 119.     CrossRef
- Biochemical and Pharmacokinetic Properties of PEGylated Cystathionine γ-Lyase from Aspergillus carneus KF723837
 Ashraf S.A. El-Sayed, Marwa A. Yassin, Salwa A. Khalaf, Mohamed El-Batrik, Gul Shad Ali, Sadik Esener
 Microbial Physiology.2015; 25(5): 301.     CrossRef
- Co-immobilization of PEGylated Aspergillus flavipes l-methioninase with glutamate dehydrogenase: A novel catalytically stable anticancer consortium
 Ashraf S.A. El-Sayed, Hend Ibrahim, Mahmoud Z. Sitohy
 Enzyme and Microbial Technology.2014; 54: 59.     CrossRef
- Methylthio-Aspochalasins from a Marine-Derived Fungus Aspergillus sp.
 Ying Liu, Shizhe Zhao, Wanjing Ding, Pinmei Wang, Xianwen Yang, Jinzhong Xu
 Marine Drugs.2014; 12(10): 5124.     CrossRef
- Advances in Detection Methods of l-Amino Acid Oxidase Activity
 Zhiliang Yu, Yangsheng Wang, Ning Zhou, Minyan Zhao, Juanping Qiu, Jianxun Lin
 Applied Biochemistry and Biotechnology.2014; 174(1): 13.     CrossRef
- Ферментные препараты в онкогематологии: актуальные направления экспериментальных исследований и перспективы клинического применения
 В. С. Покровский, Е. М. Трещалина
 Clinical Oncohematology.2014; 7(1): 28.     CrossRef
- Purification and immobilization of l-arginase from thermotolerant Penicillium chrysogenum KJ185377.1; with unique kinetic properties as thermostable anticancer enzyme
 Ashraf S. El-Sayed, Ahmed A. Shindia, Ayman A. Diab, Amgad M. Rady
 Archives of Pharmacal Research.2014;[Epub]     CrossRef
- midD-encoded ‘rhizomimosinase’ from Rhizobium sp. strain TAL1145 is a C–N lyase that catabolizes L-mimosine into 3-hydroxy-4-pyridone, pyruvate and ammonia
 Vishal Singh Negi, Jon-Paul Bingham, Qing X. Li, Dulal Borthakur
 Amino Acids.2013; 44(6): 1537.     CrossRef
- Purification and characterization of L-amino acid oxidase from the solid-state grown cultures of Aspergillus oryzae ASH
 Ashraf S. El-Sayed, Ahmed A. Shindia, Yomna A. Zaher
 Microbiology.2013; 82(6): 762.     CrossRef
- Extracellular l-Asparaginase from a Protease-Deficient Bacillus aryabhattai ITBHU02: Purification, Biochemical Characterization, and Evaluation of Antineoplastic Activity In Vitro
 Yogendra Singh, Ravi Kumar Gundampati, Medicherla V. Jagannadham, S. K. Srivastava
 Applied Biochemistry and Biotechnology.2013; 171(7): 1759.     CrossRef
- L-Amino acid oxidase from filamentous fungi: screening and optimization
 Ashraf S. El-Sayed, Ahmed A. Shindia, Yomna Zaher
 Annals of Microbiology.2012; 62(2): 773.     CrossRef
- Pharmacokinetics, immunogenicity and anticancer efficiency of Aspergillus flavipes l-methioninase
 Ashraf S. El-Sayed, Samia A. Shouman, Hatem M. Nassrat
 Enzyme and Microbial Technology.2012; 51(4): 200.     CrossRef
- Characterization and immobilization of purified Aspergillus flavipesl-methioninase: continuous production of methanethiol
 A.S. El-Sayed, A.A. Shindia
 Journal of Applied Microbiology.2011; 111(1): 54.     CrossRef