A Simple and Reliable Method for Obtaining Entomosporium Monoconidial Isolates

Mi-Jeong Park; Quinn A. Holtslag; Hyeon-Dong Shin

doi:10.1007/s12275-011-1117-y

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A Simple and Reliable Method for Obtaining Entomosporium Monoconidial Isolates: Mi-Jeong Park ¹, Quinn A. Holtslag ², Hyeon-Dong Shin ¹; Journal of Microbiology 2011;49(2):324-326.
DOI: https://doi.org/10.1007/s12275-011-1117-y
Published online: May 3, 2011

¹Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea, ²Greenspace Management, Civil Engineering Technology Department, Red River College, Manitoba, Canada

Corresponding author: Hyeon-Dong Shin , Tel: +82-2-3290-3063,

Received: 9 March 2011 • Accepted: 12 April 2011

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Abstract

Monoconidial isolates of Entomosporium mespili were successfully cultured using a simple isolation procedure. A detailed description of the steps required for isolating E. mespili is provided. The characteristic pattern of conidial germination and growth on potato dextrose agar is also described. The process that was successful in obtaining pure isolates involved collecting material in the morning, picking a glistening white mass of conidia under dissecting microscope magnification, depositing a mass of conidia onto a drop of water on a glass slide, streaking the conidial mass onto potato dextrose agar, incubating the plate for 48 h, and transferring the characteristic fan-shaped colonies to fresh medium.

Keywords: Entomosporium mespili;fan-shaped colony;germination;isolation technique

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A Simple and Reliable Method for Obtaining Entomosporium Monoconidial Isolates

J. Microbiol. 2011;49(2):324-326. Published online May 3, 2011

DOI: https://doi.org/10.1007/s12275-011-1117-y

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