A Modified Immunoblot Method to Identify Substrates of Protein Kinases

Choong-Min Kang; Wan Jin Jahng; Robert N. Husson; Sang Hee Lee

doi:10.1007/s12275-011-0465-y

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Research Support, Non-U.S. Gov't
A Modified Immunoblot Method to Identify Substrates of Protein Kinases: Choong-Min Kang ¹, Wan Jin Jahng ², Robert N. Husson ³, Sang Hee Lee ⁴; Journal of Microbiology 2011;49(3):499-501.
DOI: https://doi.org/10.1007/s12275-011-0465-y
Published online: June 30, 2011

¹Department of Biological Science, Wayne State University, 5047 Gullen Mall, Detroit, MI 48202, USA, ²Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA, ³Division of Infectious Diseases, Children’s Hospital Boston, Harvard Medical School, 300 Longwood Ave., Boston, MA 02115, USA, ⁴Department of Biological Sciences, Myongji University, Gyeonggido 449-728, Republic of Korea

Corresponding author: Choong-Min Kang , Tel: +82-31-330-6195;,
Sang Hee Lee , Tel: +82-31-330-6195;,

Received: 16 November 2010 • Accepted: 13 January 2011

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Abstract

While protein kinases are key components in multiple cellular processes, efficient identification of cognate in vivo substrates remains challenging. Here we describe a powerful method to screen potential substrates of protein kinases by partial transfer of proteins from a 2D-PAGE gel to a Western blot membrane. This approach allowed precise pinpointing of candidate substrate spots in the 2D gel, and identifying physiological substrates of protein kinases in Mycobacterium tuberculosis.

Keywords: modified immunoblot method;substrates of kinases;partial transfer of proteins

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Cardiac and pulmonary oxidative stress in rats exposed to realistic emissions of source aerosols
Miriam Lemos, Edgar A. Diaz, Tarun Gupta, Choong-Min Kang, Pablo Ruiz, Brent A. Coull, John J. Godleski, Beatriz Gonzalez-Flecha
Inhalation Toxicology.2011; 23(sup2): 75. CrossRef

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A Modified Immunoblot Method to Identify Substrates of Protein Kinases

J. Microbiol. 2011;49(3):499-501. Published online June 30, 2011

DOI: https://doi.org/10.1007/s12275-011-0465-y

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