Isolation and Characterization of Ethylbenzene Degrading Pseudomonas putida E41

Lan-Hee Kim; Sang-Seob Lee

doi:10.1007/s12275-011-0399-4

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Isolation and Characterization of Ethylbenzene Degrading Pseudomonas putida E41: Lan-Hee Kim , Sang-Seob Lee; Journal of Microbiology 2011;49(4):575-584.
DOI: https://doi.org/10.1007/s12275-011-0399-4
Published online: September 2, 2011

Department of Biotechnology, Kyonggi University, Suwon 433-760, Republic of Korea

Corresponding author: Sang-Seob Lee , Tel: +82-31-249-9642,

Received: 4 October 2010 • Accepted: 26 March 2011

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Abstract

Pseudomonas putida E41 was isolated from oil-contaminated soil and showed its ability to grow on ethylbenzene as the sole carbon and energy source. Moreover, P. putida E41 show the activity of biodegradation of ethylbenzene in the batch culture. E41 showed high efficiency of biodegradation of ethylbenzene with the optimum conditions (a cell concentration of 0.1 g wet cell weight/L, pH 7.0, 25°C, and ethylbenzene concentration of 50 mg/L) from the results of the batch culture. The maximum degradation rate and specific growth rate (μmax) under the optimum conditions were 0.19±0.03 mg/mg-DCW (Dry Cell Weight)/h and 0.87±0.13 h-1, respectively. Benzene, toluene and ethylbenzene were degraded when these compounds were provided together; however, xylene isomers persisted during degradation by P. putida E41. When using a bioreactor batch system with a binary culture with P. putida BJ10, which was isolated previously in our lab, the degradation rate for benzene and toluene was improved in BTE mixed medium (each initial concentration: 50 mg/L). Almost all of the BTE was degraded within 4 h and 70-80% of m-, p-, and o-xylenes within 11 h in a BTEX mixture (initial concentration: 50 mg/L each). In summary, we found a valuable new strain of P. putida, determined the optimal degradation conditions for this isolate and tested a mixed culture of E41 and BJ10 for its ability to degrade a common sample of mixed contaminants containing benzene, toluene, and xylene.

Keywords: ethylbenzene;Pseudomonas putida E41;characterization;biodegradation;aerobic condition

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Isolation and Characterization of Ethylbenzene Degrading Pseudomonas putida E41

J. Microbiol. 2011;49(4):575-584. Published online September 2, 2011

DOI: https://doi.org/10.1007/s12275-011-0399-4

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