Functional Definition of LuxS, an Autoinducer-2 (AI-2) Synthase and Its Role in Full Virulence of Streptococcus suis Serotype 2

Min Cao; Youjun Feng; Changjun Wang; Feng Zheng; Ming Li; Hui Liao; Yinghua Mao; Xiuzhen Pan; Jing Wang; Dan Hu; Fuquan Hu; Jiaqi Tang

doi:10.1007/s12275-011-1523-1

Articles

Page Path: HOME > J. Microbiol > Volume 49(6); 2011 > Article

Research Support, Non-U.S. Gov't
Functional Definition of LuxS, an Autoinducer-2 (AI-2) Synthase and Its Role in Full Virulence of Streptococcus suis Serotype 2: Min Cao ^1,2, Youjun Feng ^2,3, Changjun Wang ², Feng Zheng ^1,2, Ming Li ¹, Hui Liao ², Yinghua Mao ², Xiuzhen Pan ², Jing Wang ², Dan Hu ², Fuquan Hu ¹, Jiaqi Tang ^1,2,4; Journal of Microbiology 2011;49(6):1000-1011.
DOI: https://doi.org/10.1007/s12275-011-1523-1
Published online: December 28, 2011

¹Department of Microbiology, Third Military Medical University, Chongqing, P. R. China, ²Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, P. R. China, ³Clinical Microbiology & Clinical Immunology, Third Military Medical University, Chongqing, P. R. China, ⁴Department of laboratory Sciences, Nanjing General Hospital of Nanjing Military Command, Nanjing, P. R. China¹Department of Microbiology, Third Military Medical University, Chongqing, P. R. China, ²Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, P. R. China, ³Clinical Microbiology & Clinical Immunology, Third Military Medical University, Chongqing, P. R. China, ⁴Department of laboratory Sciences, Nanjing General Hospital of Nanjing Military Command, Nanjing, P. R. China

Corresponding author: Fuquan Hu , Tel: +86-023-68752834,
Jiaqi Tang , Tel: +86-025-84507094,

Received: 19 October 2011 • Accepted: 13 December 2011

prev next

44 Views
0 Download
34 Crossref
38 Scopus

Full Article

Abstract

Quorum sensing is a widespread chemical communication in response to fluctuation of bacterial population density, and has been implicated into bacterial biofilm formation and regulation of expression of virulence factors. The luxS gene product, S-ribosylhomocysteinase, catalizes the last committed step in biosynthetic pathway of autoinducer 2 (AI-2), a signaling molecule for inter-species quorum sensing. We found a luxS homologue in 05ZYH33, an epidemic strain of Streptococcus suis serotype 2 (SS2) in China. A luxS null mutant (ΔluxS) of 05ZYH33 strain was obtained using an approach of homologous recombination. LuxS was determined to be required for AI-2 production in 05ZYH33 strain of S. suis 2. Inactivation of luxS gene led to a wide range of phenotypic changes including thinner capsular walls, increased tolerance to H2O2, reduced adherence capacity to epithelial cells, etc. In particular, loss of LuxS impaired dramatically its full virulence of SS2 in experimental model of piglets, and functional complementation restored it nearly to the level of parent strain. Genome-wide transcriptome analyses suggested that some known virulence factors such as CPS are down-regulated in the ΔluxS mutant, which might in part explain virulence attenuation by luxS deletion. Similarly, 29 of 71 genes with different expression level were proposed to be targets candidate regulated by LuxS/AI-2-dependent quorum sensing.

Keywords: Streptococcus suis;Quorum sensing;Autoinducor 2 (AI-2);LuxS

Figure & Data

References

Citations

Citations to this article as recorded by

Effects of vitamin B 12 supply on cellular processes of the facultative vitamin B 12 consumer Vibrio campbellii
Luna-Agrippina Groon, Stefan Bruns, Leon Dlugosch, Heinz Wilkes, Gerrit Wienhausen, Isaac Cann
Applied and Environmental Microbiology.2025;[Epub] CrossRef
Cooperation of quorum sensing and central carbon metabolism in the pathogenesis of Gram-positive bacteria
Shuji Gao, Yuxin Wang, Shuo Yuan, Jing Zuo, Wenjie Jin, Yamin Shen, Daniel Grenier, Li Yi, Yang Wang
Microbiological Research.2024; 282: 127655. CrossRef
Collateral sensitivity to gamithromycin in ciprofloxacin-resistant Streptococcus suis is driven by increasing intracellular antibiotic accumulation
Yuejun Zhang, Mengting Tao, Ruiling Wang, Zilin Shi, Peipei Li, Sijia Tang, Jian Sun, Xiaoping Liao, Yufeng Zhou
Journal of Integrative Agriculture.2024;[Epub] CrossRef
Characterization of AI-2/LuxS quorum sensing system in biofilm formation, pathogenesis of Streptococcus equi subsp. zooepidemicus
Honglin Xie, Riteng Zhang, Ruhai Guo, Yining Zhang, Jingya Zhang, Hui Li, Qiang Fu, Xinglong Wang
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Whole-Genome Sequencing and Phenotypic Analysis of Streptococcus equi subsp. zooepidemicus Sequence Type 147 Isolated from China
Yan Su, Zehua Zhang, Li Wang, Baojiang Zhang, Lingling Su
Microorganisms.2024; 12(4): 824. CrossRef
A critical review on experimental Streptococcus suis infection in pigs with a focus on clinical monitoring and refinement strategies
Carolin Liedel, Karoline Rieckmann, Christoph G. Baums
BMC Veterinary Research.2023;[Epub] CrossRef
The Antibacterial Effect of Cannabigerol toward Streptococcus mutans Is Influenced by the Autoinducers 21-CSP and AI-2
Muna Aqawi, Ronit Vogt Sionov, Michael Friedman, Doron Steinberg
Biomedicines.2023; 11(3): 668. CrossRef
Contribution of quorum sensing to virulence and antibiotic resistance in zoonotic bacteria
Qingying Fan, Jing Zuo, Haikun Wang, Daniel Grenier, Li Yi, Yang Wang
Biotechnology Advances.2022; 59: 107965. CrossRef
mRNA-Seq reveals the quorum sensing system luxS gene contributes to the environmental fitness of Streptococcus suis type 2
Jinpeng Li, Yuxin Wang, Yanbin Du, Hui Zhang, Qingying Fan, Liyun Sun, Li Yi, Shaohui Wang, Yang Wang
BMC Microbiology.2021;[Epub] CrossRef
LsrR, the effector of AI-2 quorum sensing, is vital for the H2O2 stress response in mammary pathogenic Escherichia coli
Hui Wang, Fei Shang, Jiawei Shen, Jingyi Xu, Xiaolin Chen, Jingtian Ni, Lumin Yu, Ting Xue
Veterinary Research.2021;[Epub] CrossRef
Effect of Quorum Sensing Inhibitor Agents against Pseudomonas aeruginosa
M. Asif, M. Imran
Russian Journal of Bioorganic Chemistry.2020; 46(2): 149. CrossRef
Involvement of Various Enzymes in the Physiology and Pathogenesis of Streptococcus suis
Chengkun Zheng, Man Wei, Mengdie Jia, ManMan Cao
Veterinary Sciences.2020; 7(4): 143. CrossRef
How Streptococcus suis serotype 2 attempts to avoid attack by host immune defenses
Xiaojing Xia, Wanhai Qin, Huili Zhu, Xin Wang, Jinqing Jiang, Jianhe Hu
Journal of Microbiology, Immunology and Infection.2019; 52(4): 516. CrossRef
Quorum Sensing Inhibition: Current Advances of the Natural Antimicrobial Agents
M. Asif, M. Imran
Russian Journal of Bioorganic Chemistry.2019; 45(6): 488. CrossRef
Does the Endocannabinoid Anandamide Affect Bacterial Quorum Sensing, Vitality, and Motility?
Liat Friedman, Reem Smoum, Mark Feldman, Raphael Mechoulam, Doron Steinberg
Cannabis and Cannabinoid Research.2019; 4(2): 102. CrossRef
The LuxS/AI-2 system of Streptococcus suis
Yang Wang, Yuxin Wang, Liyun Sun, Daniel Grenier, Li Yi
Applied Microbiology and Biotechnology.2018; 102(17): 7231. CrossRef
Role of two-component regulatory systems in the virulence of Streptococcus suis
Chengkun Zheng, Lingzhi Li, Haojie Ge, Hongmei Meng, Yang Li, Weicheng Bei, Xiaohui Zhou
Microbiological Research.2018; 214: 123. CrossRef
Critical Streptococcus suis Virulence Factors: Are They All Really Critical?
Mariela Segura, Nahuel Fittipaldi, Cynthia Calzas, Marcelo Gottschalk
Trends in Microbiology.2017; 25(7): 585. CrossRef
Stress Physiology of Lactic Acid Bacteria
Konstantinos Papadimitriou, Ángel Alegría, Peter A. Bron, Maria de Angelis, Marco Gobbetti, Michiel Kleerebezem, José A. Lemos, Daniel M. Linares, Paul Ross, Catherine Stanton, Francesca Turroni, Douwe van Sinderen, Pekka Varmanen, Marco Ventura, Manuel Z
Microbiology and Molecular Biology Reviews.2016; 80(3): 837. CrossRef
Autoinducer-2 signaling is involved in regulation of stress-related genes of Deinococcus radiodurans
Lin Lin, Tao Li, Shang Dai, Jiangliu Yu, Xiuqin Chen, Liangyan Wang, Yunguang Wang, Yuejin Hua, Bing Tian
Archives of Microbiology.2016; 198(1): 43. CrossRef
Initial steps of the pathogenesis of the infection caused byStreptococcus suis: fighting against nonspecific defenses
Mariela Segura, Cynthia Calzas, Daniel Grenier, Marcelo Gottschalk
FEBS Letters.2016; 590(21): 3772. CrossRef
Crystal Structure and Identification of Two Key Amino Acids Involved in AI-2 Production and Biofilm Formation in Streptococcus suis LuxS
Yang Wang, Li Yi, Shaohui Wang, Hongjie Fan, Chan Ding, Xiang Mao, Chengping Lu, Eric Cascales
PLOS ONE.2015; 10(10): e0138826. CrossRef
First human case report of sepsis due to infection with Streptococcus suis serotype 31 in Thailand
Rujirat Hatrongjit, Anusak Kerdsin, Marcelo Gottschalk, Dan Takeuchi, Shigeyuki Hamada, Kazunori Oishi, Yukihiro Akeda
BMC Infectious Diseases.2015;[Epub] CrossRef
Two novel regulators of N‐acetyl‐galactosamine utilization pathway and distinct roles in bacterial infections
Huimin Zhang, Dmitry A. Ravcheev, Dan Hu, Fengyu Zhang, Xiufang Gong, Lina Hao, Min Cao, Dmitry A. Rodionov, Changjun Wang, Youjun Feng
MicrobiologyOpen.2015; 4(6): 983. CrossRef
Functional analysis of AI-2/LuxS from bacteria in Chinese fermented meat after high nitrate concentration shock
Mei Lin, Guang-Hong Zhou, Zhi-Geng Wang, Bai Yun
European Food Research and Technology.2015; 240(1): 119. CrossRef
Two Spx Regulators Modulate Stress Tolerance and Virulence in Streptococcus suis Serotype 2
Chengkun Zheng, Jiali Xu, Jinquan Li, Luohong Hu, Jiandong Xia, Jingyan Fan, Weina Guo, Huanchun Chen, Weicheng Bei, José A. Lemos
PLoS ONE.2014; 9(9): e108197. CrossRef
The β-galactosidase (BgaC) of the zoonotic pathogen Streptococcus suis is a surface protein without the involvement of bacterial virulence
Dan Hu, Fengyu Zhang, Huimin Zhang, Lina Hao, Xiufang Gong, Meiling Geng, Min Cao, Feng Zheng, Jin Zhu, Xiuzhen Pan, Jiaqi Tang, Youjun Feng, Changjun Wang
Scientific Reports.2014;[Epub] CrossRef
The Streptococcus suis transcriptional landscape reveals adaptation mechanisms in pig blood and cerebrospinal fluid
Zongfu Wu, Chunyan Wu, Jing Shao, Zhenzhen Zhu, Weixue Wang, Wenwei Zhang, Min Tang, Na Pei, Hongjie Fan, Jiguang Li, Huochun Yao, Hongwei Gu, Xun Xu, Chengping Lu
RNA.2014; 20(6): 882. CrossRef
Biofilm Formation, Host-Cell Adherence, and Virulence Genes Regulation of Streptococcus suis in Response to Autoinducer-2 Signaling
Yang Wang, Li Yi, Zhicheng Zhang, Hongjie Fan, Xiangchao Cheng, Chengping Lu
Current Microbiology.2014; 68(5): 575. CrossRef
Increased Constituent Ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. and a Decrease in Microflora Diversity May Be Indicators of Ventilator-Associated Pneumonia: A Prospective Study in the Respiratory Tracts of Neonates
Wei Lu, Jialin Yu, Qing Ai, Dong Liu, Chao Song, Luquan Li, Katriina Aalto-Setala
PLoS ONE.2014; 9(2): e87504. CrossRef
Streptococcus suisinfection
Youjun Feng, Huimin Zhang, Zuowei Wu, Shihua Wang, Min Cao, Dan Hu, Changjun Wang
Virulence.2014; 5(4): 477. CrossRef
Overexpression of luxS Cannot Increase Autoinducer‐2 Production, Only Affect the Growth and Biofilm Formation in Streptococcus suis
Yang Wang, Li Yi, Zhicheng Zhang, Hongjie Fan, Xiangchao Cheng, Chengping Lu, E. T. Johnson, D. Ustek
The Scientific World Journal.2013;[Epub] CrossRef
Heterologous Expression of sahH Reveals That Biofilm Formation Is Autoinducer-2-independent in Streptococcus sanguinis but Is Associated with an Intact Activated Methionine Cycle
Sylvio Redanz, Kerstin Standar, Andreas Podbielski, Bernd Kreikemeyer
Journal of Biological Chemistry.2012; 287(43): 36111. CrossRef
Attenuation of Streptococcus suis virulence by the alteration of bacterial surface architecture
Youjun Feng, Min Cao, Jie Shi, Huimin Zhang, Dan Hu, Jing Zhu, Xianyun Zhang, Meiling Geng, Feng Zheng, Xiuzhen Pan, Xianfu Li, Fuquan Hu, Jiaqi Tang, Changjun Wang
Scientific Reports.2012;[Epub] CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Functional Definition of LuxS, an Autoinducer-2 (AI-2) Synthase and Its Role in Full Virulence of Streptococcus suis Serotype 2

J. Microbiol. 2011;49(6):1000-1011. Published online December 28, 2011

DOI: https://doi.org/10.1007/s12275-011-1523-1

Related articles