Responses in the Expression of Extracellular Proteins in Methicillin-Resistant Staphylococcus aureus Treated with Rhodomyrtone

Monton Visutthi; Potjanee Srimanote; Supayang Piyawan Voravuthikunchai

doi:10.1007/s12275-011-1115-0

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Responses in the Expression of Extracellular Proteins in Methicillin-Resistant Staphylococcus aureus Treated with Rhodomyrtone: Monton Visutthi ¹, Potjanee Srimanote ², Supayang Piyawan Voravuthikunchai ^1,3; Journal of Microbiology 2011;49(6):956-964.
DOI: https://doi.org/10.1007/s12275-011-1115-0
Published online: December 28, 2011

¹Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand, ²Faculty of Allied Health Sciences, Thammasat University, Prathumthani 12120, Thailand, ³Natural Products Research Center, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand

Corresponding author: Supayang Piyawan Voravuthikunchai , Tel: +66-744-46661,

Received: 9 March 2011 • Accepted: 1 August 2011

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Abstract

Rhodomyrtone from a medicinal plant species, Rhodomyrtus tomentosa, is a challenged effective agent against Gram-positive bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA). The present study was undertaken to provide insight into MRSA extracellular protein expression following rhodomyrtone treatment. Secreteomic approach was performed on a representative clinical MRSA isolate exposing to subinhibitory concentration rhodomyrtone (0.174 μg/ml). The identified extracellular proteins of a response of MRSA to rhodomyrtone treated condition were both suppressed and overexpressed. Staphylococcal antigenic proteins, immunodominant antigen A (IsaA) and staphylococcal secretory antigen (SsaA) involved in cell wall hydrolysis were downregulated after the treatment. The results suggested that rhodomyrtone may interfere with WalK/WalR (YycG/YycF) system. Other enzymes such as lipase precursor and another lipase, glycerophosphoryl diester phosphodiesterase, were absent. In contrast, cytoplasmic proteins such as SpoVG and glycerol phosphate lipoteichoic acid synthase, and ribosomal proteins were found in the treated sample. Appearance of several cytoplasmic proteins in the treated culture supernatant revealed that the bacterial cell wall biosynthesis was disturbed. This finding provides a proteomic mapping of extracellular proteins after rhodomytone treatment. Extensive investigation is required for this natural compound as it has a great potency as an alternative anti-MRSA drug.

Keywords: Rhodomyrtus tomentosa;methicillin-resistant Staphylococcus aureus;rhodomyrtone;anti-MRSA;extracellular proteins

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Responses in the Expression of Extracellular Proteins in Methicillin-Resistant Staphylococcus aureus Treated with Rhodomyrtone

J. Microbiol. 2011;49(6):956-964. Published online December 28, 2011

DOI: https://doi.org/10.1007/s12275-011-1115-0

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