Expression of the Lactobacillus plantarum malE Gene Is Regulated by CcpA and a MalR-Like Protein

Lidia Muscariello; Valeria Vastano; Rosa A. Siciliano; Margherita Sacco; Rosangela Marasco

doi:10.1007/s12275-011-0495-5

Articles

Page Path: HOME > J. Microbiol > Volume 49(6); 2011 > Article

Research Support, Non-U.S. Gov't
Expression of the Lactobacillus plantarum malE Gene Is Regulated by CcpA and a MalR-Like Protein: Lidia Muscariello ¹, Valeria Vastano ¹, Rosa A. Siciliano ², Margherita Sacco ¹, Rosangela Marasco ³; Journal of Microbiology 2011;49(6):950-955.
DOI: https://doi.org/10.1007/s12275-011-0495-5
Published online: December 28, 2011

¹Dipartimento di Scienze Ambientali, Seconda Università di Napoli, via Vivaldi 43, 81100 Caserta, Italy, ²Centro di Spettrometria di Massa Proteomica e Biomolecolare, Istituto di Scienze dell’Alimentazione, CNR, Avellino, Italy, ³Dipartimento di Scienze della Vita, Seconda Università di Napoli, via Vivaldi 43, 81100 Caserta, Italy

Corresponding author: Rosangela Marasco , Tel: +39-0823-274557,

Received: 2 December 2010 • Accepted: 15 July 2011

prev next

278 Views
0 Download
12 Crossref
13 Scopus

Full Article

Download PDF

Abstract

Lactobacillus plantarum is commonly used in the food industry as a starter in various fermentations, especially in vegetable fermentations, in which starch is a common substrate. This polysaccharide, which is obtained from potatoes or corn and is hydrolysed mainly to maltose and glucose by acids or enzymes, is commercially used for the production of lactate by lactic acid fermentation. In this study, we describe the regulation of malE gene expression in L. plantarum. This gene, located in a 7-gene cluster, probably organized in an operon, encodes a putative maltose/maltodextrin-binding protein. We studied the expression of malE in L. plantarum LM3 (wild type) and in LM3-2 (ccpA1), which carries a null mutation in the ccpA gene, encoding the catabolite control protein A (CcpA). In the presence of glucose, expression of the MalE protein was higher in the mutant strain as compared to that in the wild-type strain. Transcription of the malE gene was induced by maltose and regulated by a CcpA-mediated carbon catabolite repression. Further, we isolated strains carrying mutations in 2 genes, lp_0172 and lp_0173, whose deduced amino acid sequences share significant identity with MalR, a regulator of the maltose operon in several gram-positive bacteria. A double mutant exhibited glucose-insensitive malE transcription, while absence of the functional Lp_0172 open reading frame had no effect on malE expression.

Keywords: maltose/maltodextrin;Lactobacillus plantarum;catabolite control protein A;MalR

Figure & Data

References

Citations

Citations to this article as recorded by

CcpA-mediated regulation of cellular energy metabolism in the ruminal bacterium Streptococcus bovis
Yunan Weng, Yaqian Jin, Wenze Zhang, Lihong Wang, Hongrong Wang, Zhongxiong Lai
Microbiology Spectrum.2025;[Epub] CrossRef
Systematic elucidation of independently modulated genes in Lactiplantibacillus plantarum reveals a trade‐off between secondary and primary metabolism
Sizhe Qiu, Yidi Huang, Shishun Liang, Hong Zeng, Aidong Yang
Microbial Biotechnology.2024;[Epub] CrossRef
Analyses of cellular responses of phorate-incubated Lactiplantibacillus plantarum by transcriptomics and proteomics
Jingna Zhao, Lai-Yu Kwok, Hui Fan, Xiufang Liu, Yongfu Chen
LWT.2023; 189: 115443. CrossRef
Global transcriptomic analysis of Lactobacillus plantarum CAUH2 in response to hydrogen peroxide stress
Zhengyuan Zhai, Yang Yang, Hui Wang, Guohong Wang, Fazheng Ren, Zaigui Li, Yanling Hao
Food Microbiology.2020; 87: 103389. CrossRef
Comparative Transcriptional Analysis of Lactobacillus plantarum and Its ccpA-Knockout Mutant Under Galactooligosaccharides and Glucose Conditions
Chen Chen, Linlin Wang, Yanqing Lu, Haiyan Yu, Huanxiang Tian
Frontiers in Microbiology.2019;[Epub] CrossRef
Technological characterization of vaginal probiotic lactobacilli: resistance to osmotic stress and strains compatibility
J.A. Silva, A. Marchesi, B. Wiese, M.E.F. Nader‐Macias
Journal of Applied Microbiology.2019; 127(6): 1835. CrossRef
Stress Physiology of Lactic Acid Bacteria
Konstantinos Papadimitriou, Ángel Alegría, Peter A. Bron, Maria de Angelis, Marco Gobbetti, Michiel Kleerebezem, José A. Lemos, Daniel M. Linares, Paul Ross, Catherine Stanton, Francesca Turroni, Douwe van Sinderen, Pekka Varmanen, Marco Ventura, Manuel Z
Microbiology and Molecular Biology Reviews.2016; 80(3): 837. CrossRef
Catabolite responsive element deficiency ofxyloperon resulting in carbon catabolite derepression inLactobacillus fermentum1001
C. Zhang, T. Guo, Y. Xin, X. Gao, J. Kong
Journal of Applied Microbiology.2016; 120(1): 126. CrossRef
Transcriptional Profile during Deoxycholate-Induced Sporulation in a Clostridium perfringens Isolate Causing Foodborne Illness
Mayo Yasugi, Daisuke Okuzaki, Ritsuko Kuwana, Hiromu Takamatsu, Masaya Fujita, Mahfuzur R. Sarker, Masami Miyake, C. M. Dozois
Applied and Environmental Microbiology.2016; 82(10): 2929. CrossRef
Comparative proteomic analysis of biofilm and planktonic cells of Lactobacillus plantarum DB200
Maria De Angelis, Sonya Siragusa, Daniela Campanella, Raffaella Di Cagno, Marco Gobbetti
PROTEOMICS.2015; 15(13): 2244. CrossRef
Fermentation and proteome profiles of Lactobacillus plantarum strains during growth under food-like conditions
Sonya Siragusa, Maria De Angelis, Maria Calasso, Daniela Campanella, Fabio Minervini, Raffaella Di Cagno, Marco Gobbetti
Journal of Proteomics.2014; 96: 366. CrossRef
Effect of inactivation of ccpA and aerobic growth in Lactobacillus plantarum: A proteomic perspective
Maria F. Mazzeo, Giuseppina Cacace, Angela Peluso, Teresa Zotta, Lidia Muscariello, Valeria Vastano, Eugenio Parente, Rosa A. Siciliano
Journal of Proteomics.2012; 75(13): 4050. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Expression of the Lactobacillus plantarum malE Gene Is Regulated by CcpA and a MalR-Like Protein

J. Microbiol. 2011;49(6):950-955. Published online December 28, 2011

DOI: https://doi.org/10.1007/s12275-011-0495-5

Related articles