Effects of Phosphate Addition on Biofilm Bacterial Communities and Water Quality in Annular Reactors Equipped with Stainless Steel and Ductile Cast Iron Pipes

Hyun-Jung Jang; Young-June Choi; Hee-Myong Ro; Jong-Ok Ka

doi:10.1007/s12275-012-1040-x

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Research Support, Non-U.S. Gov't
Effects of Phosphate Addition on Biofilm Bacterial Communities and Water Quality in Annular Reactors Equipped with Stainless Steel and Ductile Cast Iron Pipes: Hyun-Jung Jang ^1,2, Young-June Choi ¹, Hee-Myong Ro ², Jong-Ok Ka ^2,3; Journal of Microbiology 2012;50(1):17-28.
DOI: https://doi.org/10.1007/s12275-012-1040-x
Published online: February 27, 2012

¹Division of R&D for Water, Waterworks Research Institute, Seoul 143-820, Republic of Korea, ²Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea, ³Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea

Corresponding author: Jong-Ok Ka , Tel: +82-2-880-4673,

Received: 24 January 2011 • Accepted: 16 September 2011

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Abstract

The impact of orthophosphate addition on biofilm formation and water quality was studied in corrosion-resistant stainless steel (STS) pipe and corrosion-susceptible ductile cast iron (DCI) pipe using cultivation and culture-independent approaches. Sample coupons of DCI pipe and STS pipe were installed in annular reactors, which were operated for 9 months under hydraulic conditions similar to a domestic plumbing system. Addition of 5 mg/L of phosphate to the plumbing systems, under low residual chlorine conditions, promoted a more significant growth of biofilm and led to a greater rate reduction of disinfection by-products in DCI pipe than in STS pipe. While the level of THMs (trihalomethanes) increased under conditions of low biofilm concentration, the levels of HAAs (halo acetic acids) and CH (chloral hydrate) decreased in all cases in proportion to the amount of biofilm. It was also observed that chloroform, the main species of THM, was not readily decomposed biologically and decomposition was not proportional to the biofilm concentration; however, it was easily biodegraded after the addition of phosphate. Analysis of the 16S rDNA sequences of 102 biofilm isolates revealed that Proteobacteria (50%) was the most frequently detected phylum, followed by Firmicutes (10%) and Actinobacteria (2%), with 37% of the bacteria unclassified. Bradyrhizobium was the dominant genus on corroded DCI pipe, while Sphingomonas was predominant on non-corroded STS pipe. Methylobacterium and Afipia were detected only in the reactor without added phosphate. PCR-DGGE analysis showed that the diversity of species in biofilm tended to increase when phosphate was added regardless of the pipe material, indicating that phosphate addition upset the biological stability in the plumbing systems.

Keywords: biofilm;phosphate;disinfection by-products;drinking water distribution system;stainless steel pipe;ductile cast iron pipe

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Effects of Phosphate Addition on Biofilm Bacterial Communities and Water Quality in Annular Reactors Equipped with Stainless Steel and Ductile Cast Iron Pipes

J. Microbiol. 2012;50(1):17-28. Published online February 27, 2012

DOI: https://doi.org/10.1007/s12275-012-1040-x

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