Porphyromonas gingivalis-Derived Lipopolysaccharide-Mediated Activation of MAPK Signaling Regulates Inflammatory Response and Differentiation in Human Periodontal Ligament Fibroblasts

Taegun Seo; Seho Cha; Tae-Il Kim; Hee-Jung Park; Jeong-Soon Lee; Kyung Mi Woo

doi:10.1007/s12275-012-2146-x

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Porphyromonas gingivalis-Derived Lipopolysaccharide-Mediated Activation of MAPK Signaling Regulates Inflammatory Response and Differentiation in Human Periodontal Ligament Fibroblasts: Taegun Seo ¹, Seho Cha ¹, Tae-Il Kim ², Hee-Jung Park ², Jeong-Soon Lee ², Kyung Mi Woo ³; Journal of Microbiology 2012;50(2):311-319.
DOI: https://doi.org/10.1007/s12275-012-2146-x
Published online: April 27, 2012

¹Department of Life Science, Dongguk University-Seoul, Seoul 100-715, Republic of Korea, ²Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry, Seoul 110-749, Republic of Korea, ³Department of Cell and Developmental Biology, Seoul National University School of Dentistry, Seoul 110-749, Republic of Korea¹Department of Life Science, Dongguk University-Seoul, Seoul 100-715, Republic of Korea, ²Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry, Seoul 110-749, Republic of Korea, ³Department of Cell and Developmental Biology, Seoul National University School of Dentistry, Seoul 110-749, Republic of Korea

Corresponding author: Tae-Il Kim , Tel: +82-2-2072-2642,

Received: 15 March 2012 • Accepted: 6 April 2012

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Abstract

Porphyromonas gingivalis (P.g.), which is a potential pathogen for periodontal diseases, contains lipopolysaccharide (LPS), and this endotoxin stimulates a variety of cellular responses. At present, P.g.-derived LPS-induced cellular responses in human periodontal ligament fibroblasts (PDLFs) are not well characterized. Here, we demonstrate that P.gderived LPS regulates inflammatory responses, apoptosis and differentiation in PDLFs. Interleukin-6 (IL-6) and -8 (IL-8) were effectively upregulated by treatment of P.g.-derived LPS, and we confirmed apoptosis markers including elevated cytochrome c levels, active caspase-3 and morphological change in the presence of P.g.-derived LPS. Moreover, when PDLFs were cultured with differentiation media, P.g.- derived LPS reduced the expression of differentiation marker genes, as well as reducing alkaline phosphatase (ALP) activity and mineralization. P.g.-derived LPS-mediated these cellular responses were effectively abolished by treatment of mitogen-activated protein kinase (MAPK) inhibitors. Taken together, our results suggest that P.g.-derived LPS regulates several cellular responses via activation of MAPK signaling pathways in PDLFs.

Keywords: apoptosis;cell differentiation;lipopolysaccharide;mitogen-activated protein kinase;periodontal ligament;Porphyromonas gingivalis

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Porphyromonas gingivalis-Derived Lipopolysaccharide-Mediated Activation of MAPK Signaling Regulates Inflammatory Response and Differentiation in Human Periodontal Ligament Fibroblasts

J. Microbiol. 2012;50(2):311-319. Published online April 27, 2012

DOI: https://doi.org/10.1007/s12275-012-2146-x

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