MSK
Articles
- Page Path
- HOME > J. Microbiol > Volume 50(4); 2012 > Article
-
Research Support, Non-U.S. Gov't
- Novel Bifidobacterium Promoters Selected Through Microarray Analysis Lead to Constitutive High-Level Gene Expression
- Yan Wang 1, Jin Yong Kim 1, Myeong Soo Park 2,3, Geun Eog Ji 1,3
-
Journal of Microbiology 2012;50(4):638-643.
DOI: https://doi.org/10.1007/s12275-012-1591-x
Published online: July 21, 2012
-
Corresponding author:
Myeong Soo Park , Tel: +82-2-880-8749/+82-31-441-1347,
Geun Eog Ji , Tel: +82-2-880-8749/+82-31-441-1347,
Abstract
For the development of a food-grade expression system for Bifidobacterium, a strong promoter leading to high-level expression of cloned gene is a prerequisite. For this purpose, a promoter screening host-vector system for Bifidobacterium has been established using β-glucosidase from Bifidobacterium lactis as a reporter and Bifidobacterium bifidum BGN4 as a host, which is β-glucosidase negative strain. Seven putative promoters showing constitutive high-level expression were selected through microarray analysis based on the genome sequence of B. bifidum BGN4. They were cloned into upstream of β-glucosidase gene and transformed into Escherichia coli DH5α and B. bifidum BGN4. Promoter activities were analyzed both in E. coli and B. bifidum BGN4 by measuring β-glucosidase activity. β-Glucosidase activities in all of the transformants showed growth-associated characteristics. Among them, P919 was the strongest in B. bifidum BGN4 and showed maximum activity at 18 h, while P895 was the strongest in E. coli DH5α at 7 h. This study shows that novel strong promoters such as P919 can be used for high-level expression of foreign genes in Bifidobacterium and will be useful for the construction of an efficient food-grade expression system.
-
Cite this Article
- Cite this Article
-
- Close
- Download Citation
- Close
- Related articles
-
TOP
