Involvement of Alternative Oxidase in the Regulation of Growth, Development, and Resistance to Oxidative Stress of Sclerotinia sclerotiorum

Ting Xu; Fei Yao; Wu-Sheng Liang; Yong-Hong Li; Dian-Rong Li; Hao Wang; Zheng-Yi Wang

doi:10.1007/s12275-012-2015-7

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Research Support, Non-U.S. Gov't
Involvement of Alternative Oxidase in the Regulation of Growth, Development, and Resistance to Oxidative Stress of Sclerotinia sclerotiorum: Ting Xu ^1,2, Fei Yao ^1,2, Wu-Sheng Liang ^1,2, Yong-Hong Li ³, Dian-Rong Li ³, Hao Wang ³, Zheng-Yi Wang ^1,2; Journal of Microbiology 2012;50(4):594-602.
DOI: https://doi.org/10.1007/s12275-012-2015-7
Published online: August 25, 2012

¹Institute of Biotechnology, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, P. R. China, ²State Key Laboratory of Rice Biology, Hangzhou 310058, P. R. China, ³Hybrid Rapeseed Research Center of Shaanxi Province, Dali 715105, P. R. China¹Institute of Biotechnology, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, P. R. China, ²State Key Laboratory of Rice Biology, Hangzhou 310058, P. R. China, ³Hybrid Rapeseed Research Center of Shaanxi Province, Dali 715105, P. R. China

Corresponding author: Wu-Sheng Liang , Tel: +86-571-88982261,

Received: 11 January 2012 • Accepted: 26 May 2012

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Abstract

Sclerotinia sclerotiorum is a cosmopolitan, filamentous, fungal pathogen that can cause serious disease in many kinds of crops. Alternative oxidase is the terminal oxidase of the alternative mitochondrial respiratory pathway in fungi and higher plants. We report the presence of this alternative pathway respiration and demonstrate its expression in two isolates of S. sclerotiorum under unstressed, normal culture conditions. Application of salicylhydroxamic acid, a specific inhibitor of alternative oxidase, severely inhibited the mycelial growth of S. sclerotiorum both on potato dextrose agar plates and in liquid culture media. Inhibition of alternative oxidase could influence the growth pattern of S. sclerotiorum, as salicylhydroxamic acid treatment induced obvious aerial mycelia growing on potato dextrose agar plates. Under the treatment with salicylhydroxamic acid, S. sclerotiorum formed sclerotia much more slowly than the control. Treatment with hydrogen peroxide in millimolar concentrations greatly decreased the growth rate of mycelia and delayed the formation of sclerotia in both tested S. sclerotiorum isolates. As well, this treatment obviously increased their alternative pathway respiration and the levels of both mRNA and protein of the alternative oxidase. These results indicate that alternative oxidase is involved in the regulation of growth, development, and resistance to oxidative stress of S. sclerotiorum.

Keywords: Sclerotinia sclerotiorum;alternative oxidase;mitochondrial respiratory chain;hydrogen peroxide;oxidative stress

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Involvement of Alternative Oxidase in the Regulation of Growth, Development, and Resistance to Oxidative Stress of Sclerotinia sclerotiorum

J. Microbiol. 2012;50(4):594-602. Published online August 25, 2012

DOI: https://doi.org/10.1007/s12275-012-2015-7

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