In Vitro Development and Transfer of Resistance to Chlortetracycline in Bacillus subtilis

Menghong Dai; Junjie Lu; Yulian Wang; Zhenli Liu; Zonghui Yuan

doi:10.1007/s12275-012-1454-5

Articles

Page Path: HOME > J. Microbiol > Volume 50(5); 2012 > Article

Research Support, Non-U.S. Gov't
In Vitro Development and Transfer of Resistance to Chlortetracycline in Bacillus subtilis: Menghong Dai , Junjie Lu , Yulian Wang , Zhenli Liu , Zonghui Yuan; Journal of Microbiology 2012;50(5):807-812.
DOI: https://doi.org/10.1007/s12275-012-1454-5
Published online: November 4, 2012

MOA Key Laboratory of Food Safety Evaluation / National Reference Laboratory of Veterinary Drug Residue (HZAU), Huazhong Agricultural University, Wuhan, Hubei 430070, P. R. China

Corresponding author: Zonghui Yuan , Tel: +86-027-8728-7186,

Received: 14 September 2011 • Accepted: 29 June 2012

prev next

193 Views
0 Download
12 Crossref
19 Scopus

Full Article

Abstract

The present criteria and rules controlling the approval of the use of probiotics are limited to antibiotic resistance patterns and the presence of antibiotic resistance genes in bacteria. There is little information available in the literature regarding the risk of the usage of probiotics in the presence of antibiotic pressure. In this study we investigated the development and transfer of antibiotic resistance in Bacillus subtilis selected in vitro by chlortetracycline in a stepwise manner. Bacillus subtilis was exposed to increasing concentrations of chlortetracyclineto induce in vitro resistance to chlortetracycline, and the minimal inhibitory concentrations were determinedfor the mutants. Resistant B. subtilis were conjugated with Escherichia coli NK5449 and Enterococcus faecalis JH2-2 using the filter mating. Three B. subtilis tetracycline resistant mutants (namely, BS-1, BS-2, and BS-3) were derived in vitro. A tetracycline resistant gene, tet (K), was found in the plasmids of BS-1 and BS-2. Three conjugates (BS-1N, BS-2N, and BS-3N) were obtained when the resistant B. subtilis was conjugated with E. coli NK5449. The conjugation frequencies for the BS-1N, BS-2N, and BS-3N conjugates were 4.57×10-7, 1.4×10-7, and 1.3×10-8, respectively. The tet(K) gene was found only in the plasmids of BS-1N. These results indicate that long-term use of probiotics under antibiotic selection pressure could cause antibiotic resistance, and the resistance gene could be transferred to other bacteria. The risk arising from the use of probiotics under antibiotic pressure should be considered in the criteria and rules for the safety assessment of probiotics.

Keywords: Bacillus subtilis;chlortetracycline;antibiotic resistance;conjugation transfer;tet(K);minimal inhibitory concentrations

Figure & Data

References

Citations

Citations to this article as recorded by

Searching, identifying and characterizing antagonistic isolates to control Neofabraea representatives causing bull’s-eye rot in apples
Klaudia Zawadzka, Karolina Oszust, Michał Pylak, Agata Gryta, Jacek Panek, Tomasz Lipa, Magdalena Frąc
International Agrophysics.2025; 39(2): 145. CrossRef
Antibiotic Resistance Gene Expression in Veterinary Probiotics: Two Sides of the Coin
Ádám Kerek, István Román, Ábel Szabó, Nikolett Palkovicsné Pézsa, Ákos Jerzsele
Veterinary Sciences.2025; 12(3): 217. CrossRef
Probiyotiklerin Genel Özellikleri ve Sağlık Üzerine Etkileri
Batuhan Horasan, Nevzat Atalay Çelikyürek
Sağlık Akademisi Kastamonu.2024; 9(2): 345. CrossRef
Tetracyclines resistance in Mycoplasma and Ureaplasma urogenital isolates derived from human: a systematic review and meta-analysis
Xiaoyan Wen, Mojgan Sarabi Nobakht, Yue Yang, Ebrahim Kouhsari, Sara Hajilari, Matin Zafar Shakourzadeh, Khalil Azizian
Annals of Clinical Microbiology and Antimicrobials.2023;[Epub] CrossRef
Bacillus Species as Direct-Fed Microbial Antibiotic Alternatives for Monogastric Production
Shifa A. Bahaddad, Meshal H. K. Almalki, Othman A. Alghamdi, Sayed S. Sohrab, Muhammad Yasir, Esam I. Azhar, Hichem Chouayekh
Probiotics and Antimicrobial Proteins.2023; 15(1): 1. CrossRef
Bacillusspore-forming probiotics: benefits with concerns?
Svetoslav Dimitrov Todorov, Iskra Vitanova Ivanova, Igor Popov, Richard Weeks, Michael Leonidas Chikindas
Critical Reviews in Microbiology.2022; 48(4): 513. CrossRef
Exploring the persistence and spreading of antibiotic resistance from manure to biocompost, soils and vegetables
Chengjun Pu, Yao Yu, Jianxiong Diao, Xiaoyan Gong, Ji Li, Ying Sun
Science of The Total Environment.2019; 688: 262. CrossRef
Bacillusprobiotics: an alternative to antibiotics for livestock production
S. Mingmongkolchai, W. Panbangred
Journal of Applied Microbiology.2018; 124(6): 1334. CrossRef
Distinguishing Effects of Ultraviolet Exposure and Chlorination on the Horizontal Transfer of Antibiotic Resistance Genes in Municipal Wastewater
Mei-Ting Guo, Qing-Bin Yuan, Jian Yang
Environmental Science & Technology.2015; 49(9): 5771. CrossRef
Probiotics: an update
Yvan Vandenplas, Geert Huys, Georges Daube
Jornal de Pediatria (Versão em Português).2015; 91(1): 6. CrossRef
Probiotics: an update
Yvan Vandenplas, Geert Huys, Georges Daube
Jornal de Pediatria.2015; 91(1): 6. CrossRef
Probiotics and Prebiotics in Infants and Children
Y. Vandenplas, E. De Greef, T. Devreker, G. Veereman-Wauters, B. Hauser
Current Infectious Disease Reports.2013; 15(3): 251. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

In Vitro Development and Transfer of Resistance to Chlortetracycline in Bacillus subtilis

J. Microbiol. 2012;50(5):807-812. Published online November 4, 2012

DOI: https://doi.org/10.1007/s12275-012-1454-5

Related articles