Screening of Mutant Strain Streptomyces mediolani sp. AC37 for (-)-8-O-Methyltetrangomycin Production Enhancement

Jakeline Trejos Jiménez; Maria Sturdíková; Vlasta Brezová; Emil Svajdlenka; Marta Novotová

doi:10.1007/s12275-012-2025-5

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Research Support, Non-U.S. Gov't
Screening of Mutant Strain Streptomyces mediolani sp. AC37 for (-)-8-O-Methyltetrangomycin Production Enhancement: Jakeline Trejos Jiménez ¹, Maria Sturdíková ¹, Vlasta Brezová ², Emil Svajdlenka ³, Marta Novotová ⁴; Journal of Microbiology 2012;50(6):1014-1023.
DOI: https://doi.org/10.1007/s12275-012-2025-5
Published online: December 30, 2012

¹Institute of Biotechnology and Food Science, Slovak University of Technology in Bratislava, Radlinského 9, SK-812 37 Bratislava, Slovak Republic, ²Institute of Physical Chemistry and Chemical Physics, Slovak University of Technology in Bratislava, Radlinského 9, SK-812 37 Bratislava, Slovak Republic, ³BEL/NOVAMANN International s.r.o, Komjatická 73, 940 02 Nové Zámky, Slovak Republic, ⁴Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlárska 5, SK-833 34 Bratislava, Slovak Republic¹Institute of Biotechnology and Food Science, Slovak University of Technology in Bratislava, Radlinského 9, SK-812 37 Bratislava, Slovak Republic, ²Institute of Physical Chemistry and Chemical Physics, Slovak University of Technology in Bratislava, Radlinského 9, SK-812 37 Bratislava, Slovak Republic, ³BEL/NOVAMANN International s.r.o, Komjatická 73, 940 02 Nové Zámky, Slovak Republic, ⁴Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlárska 5, SK-833 34 Bratislava, Slovak Republic

Corresponding author: Jakeline Trejos Jiménez , Tel: +421-2-59325-712,

Received: 18 January 2012 • Accepted: 20 July 2012

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Abstract

Streptomyces mediolani sp. AC37 was isolated from the root system of higher plant Taxus baccata and produced metabolite identified as (-)-8-O-methyltetrangomycin according to LC/MS/MS analysis. In our screening program for improvements of bioactive secondary metabolites from plant associate streptomycetes, mutation was used as a tool for the induction of genetic variations for selection of higher (-)-8-O-methyltetrangomycin producers of isolates. S. mediolani sp. AC37 was treated with UV irradiation and chemical mutagenic treatment (N-nitroso-N-methyl-urea). The radical scavenging and antioxidant capacity of (-)-8-O-methyltetrangomycin and extracts isolated from mutants were tested using EPR spin trapping technique and ABTS􀁹+ assay. Comparison of electron microscopic images of Streptomyces sp. AC37 and mutant strains of Streptomyces sp. AC37 revealed substantial differences in morphology and ultrastructure.

Keywords: Streptomyces mediolani;(-)-8-O-methyltetrangomycin;mutation;N-nitroso-N-methyl-urea;EPR spectroscopy;sulphate radical anion;antioxidant

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Screening of Mutant Strain Streptomyces mediolani sp. AC37 for (-)-8-O-Methyltetrangomycin Production Enhancement

J. Microbiol. 2012;50(6):1014-1023. Published online December 30, 2012

DOI: https://doi.org/10.1007/s12275-012-2025-5

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