Identification of Petriella setifera LH and Characterization of Its Crude Carboxymethyl Cellulase for Application in Denim Biostoning

Xi-Hua Zhao; Wei Wang; Dong-Zhi Wei

doi:10.1007/s12275-013-2370-z

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Identification of Petriella setifera LH and Characterization of Its Crude Carboxymethyl Cellulase for Application in Denim Biostoning: Xi-Hua Zhao ^1,2, Wei Wang ¹, Dong-Zhi Wei ¹; Journal of Microbiology 2013;51(1):82-87.
DOI: https://doi.org/10.1007/s12275-013-2370-z
Published online: March 2, 2013

¹State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, P. R. China, ²College of Life Science, Jiangxi Normal University, Nanchang 330022, P. R. China

Corresponding author: Wei Wang , Tel: +86-21-64253287,
Dong-Zhi Wei , Tel: +86-21-64253287,

Received: 19 July 2012 • Accepted: 3 October 2012

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Abstract

The phylogenetic tree of the partial elongation factor-1 alpha gene fits better than the partial 18S rDNA for generic classification. From the results of the molecular tree and analysis of morphological characters, Petriella setifera LH was identified. It can be induced to produce carboxymethyl cellulase (CMCase). The crude CMCase only shows a 44.1-kDa band by activity staining after SDS-PAGE. It is optimally active at 55°C and pH 6.0, and is stable from pH 5.0–8.0 and at 45°C or below. The crude CMCase, which is not affected by Co2+, is strongly activated in the presence of 10 mM Na+, K+, Ca2+, Mg2+, EDTA, and Mn2+. It is strongly inhibited by 10 mM Fe2+, Pb2+, Al3+, Zn2+, Ag+, Fe3+, and Cu2+. When compared with denim treatment by Novoprime A800 (a commercial neutral cellulase), crude CMCase exhibits a similar fabric weight loss and indigo dye removal. These results indicate that crude CMCase has potential application in denim biostoning.

Keywords: neutral carboxymethyl cellulase;Petriella setifera LH;biostoning

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Identification of Petriella setifera LH and Characterization of Its Crude Carboxymethyl Cellulase for Application in Denim Biostoning

J. Microbiol. 2013;51(1):82-87. Published online March 2, 2013

DOI: https://doi.org/10.1007/s12275-013-2370-z

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