Quantification of Toxigenic Microcystis spp. in Freshwaters by Quantitative Real-time PCR Based on the Microcystin Synthetase A Gene

Kyoung-Hee Oh; Dong-Hwan Jeong; Young-Cheol Cho

doi:10.1007/s12275-013-2354-z

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Quantification of Toxigenic Microcystis spp. in Freshwaters by Quantitative Real-time PCR Based on the Microcystin Synthetase A Gene: Kyoung-Hee Oh ¹, Dong-Hwan Jeong ², Young-Cheol Cho ¹; Journal of Microbiology 2013;51(1):18-24.
DOI: https://doi.org/10.1007/s12275-013-2354-z
Published online: March 2, 2013

¹Department of Environmental Engineering, Chungbuk National University, Cheongju 361-763, Republic of Korea, ²National Institute of Environmental Research, Incheon 404-708, Republic of Korea

Corresponding author: Young-Cheol Cho , Tel: +82-43-261-3577,

Received: 10 July 2012 • Accepted: 16 November 2012

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Abstract

A method to estimate the abundance of toxigenic Microcystis in environmental samples by using quantitative real-time PCR was developed and optimized. The basis of this method is the amplification of a highly conserved region of the mcyA gene within the microcystin synthetase gene cluster. Using this method, the average copy number of mcyA gene per cell in toxigenic Microcystis strains was estimated. The molecular markers and method developed in this study can be used to monitor toxigenic strains of Microcystis in Korean freshwaters, in which harmful cyanobacterial blooms are routinely found.

Keywords: toxic Microcystis;microcystins;quantitative realtime PCR;microcystin synthetase gene

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Quantification of Toxigenic Microcystis spp. in Freshwaters by Quantitative Real-time PCR Based on the Microcystin Synthetase A Gene

J. Microbiol. 2013;51(1):18-24. Published online March 2, 2013

DOI: https://doi.org/10.1007/s12275-013-2354-z

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