An Aqueous Extract of Yunnan Baiyao Inhibits the Quorum-Sensing-Related Virulence of Pseudomonas aeruginosa

Zu-Guo Zhao; Shuang-Shuang Yan; Yun-Mei Yu; Na Mi; La-Xi Zhang; Jun Liu; Xiao-Ling Li; Fang Liu; Jun-Fa Xu; Wei-Qing Yang; Guo-Ming Li

doi:10.1007/s12275-013-2595-x

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An Aqueous Extract of Yunnan Baiyao Inhibits the Quorum-Sensing-Related Virulence of Pseudomonas aeruginosa: Zu-Guo Zhao ¹, Shuang-Shuang Yan ², Yun-Mei Yu ³, Na Mi ¹, La-Xi Zhang ³, Jun Liu ¹, Xiao-Ling Li ¹, Fang Liu ¹, Jun-Fa Xu ², Wei-Qing Yang ¹, Guo-Ming Li ¹; Journal of Microbiology 2013;51(2):207-212.
DOI: https://doi.org/10.1007/s12275-013-2595-x
Published online: April 27, 2013

¹Department of Microbiology and Immunology, Guangdong Medical College, Zhanjiang, 524023, P. R. China, ²Institute of Medical Laboratory, Guangdong Medical College, Dongguan, 523808, P. R. China, ³The 422nd Central Hospital, the People’s Liberation Army, Zhanjiang, 524023, P. R. China

Corresponding author: Guo-Ming Li , Tel: +867592388270,

Received: 30 October 2012 • Accepted: 30 December 2012

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Abstract

Yunnan Baiyao is a famous Chinese medicine that has long been directly applied to wounds to reduce bleeding, pain, and swelling without causing infection. However, little is known about its ability to prevent infection. The present study aimed to assess in vitro the anti-virulence activity of an aqueous extract of Yunnan Baiyao (YBX) using Pseudomonas aeruginosa as a pathogenic model. We found that a sub-MIC (2.5 mg/ml) of YBX can efficiently interfere with the quorum-sensing (QS) signaling circuit. Real-time polymerase chain reaction analysis showed that a sub-MIC of YBX downregulated the transcriptions of lasR, lasI, rhlR, and rhlI, which resulted in global attenuation of QS-regulated virulence activities, such as biofilm formation, and secretion of LasA protease, LasB elastase and pyocyanin. Further, YBX reduced the motility of P. aeruginosa related to QS, and impaired the formation of biofilms. These results suggest that YBX may possess global inhibitory activity against the virulence of P. aeruginosa and that YBX may also exhibit antimicrobial activity in vivo. The present study suggests that Yunnan Baiyao represents a potential source for isolating novel, safe, and efficacious antimicrobial agents.

Keywords: P. aeruginosa;quorum sensing;virulence;motility;Yunnan Baiyao

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An Aqueous Extract of Yunnan Baiyao Inhibits the Quorum-Sensing-Related Virulence of Pseudomonas aeruginosa

J. Microbiol. 2013;51(2):207-212. Published online April 27, 2013

DOI: https://doi.org/10.1007/s12275-013-2595-x

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