Abstract
Fungal cell walls consist of various glucans and chitin. The inky cap, Coprinellus congregatus, produces mushrooms at 25°C in a regime of 15 h light/9 h dark, and then the mushroom is autolyzed rapidly to generate black liquid droplets in which no cell walls are detected by microscopy. Chitinase cDNA from the mature mushroom tissues of C. congregatus, which consisted of 1,622 nucleotides (chi2), was successfully cloned using the rapid amplification of cDNA ends polymerase chain reaction technique. The deduced 498 amino acid sequence of Chi2 had a conserved catalytic domain as in other fungal chitinase family 18 enzymes. The Chi2 enzyme
was purified from the Pichia pastoris expression system, and its estimated molecular weight was 68 kDa. The optimum pH and temperature of Chi2 was pH 4.0 and 35°C,
respectively when 4-nitrophenyl N,N’-diacetyl-β-D-chitobioside was used as the substrate. The Km value and Vmax for the substrate A, 4-nitrophenyl N,N’-diacetyl-β-D-chitobioside, was 0.175 mM and 0.16 OD min-1unit-1, respectively.
Citations
Citations to this article as recorded by

- Chitin Synthases in Cordyceps militaris: Genome-Wide Gene Identification, Evolutionary Insights, and Life Cycle Transcript Profiling
Shoumian Li, Huihui Ren, Jie Zhang, Shangpai Gao, Zixuan Chen, Guojie Li, Jinghua Tian, Junling Wang, Ming Li, Xiao Li, Caihong Dong
Horticulturae.2024; 10(5): 494. CrossRef - Chitinase Is Involved in the Fruiting Body Development of Medicinal Fungus Cordyceps militaris
Zi-Juan Zhang, Yuan-Yuan Yin, Yao Cui, Yue-Xuan Zhang, Bi-Yang Liu, You-Chu Ma, Yong-Nan Liu, Gao-Qiang Liu
Life.2023; 13(3): 764. CrossRef - Myco-chitinases as versatile biocatalysts for translation of coastal residual resources to eco-competent chito-bioactives
Meenakshi Rajput, Manish Kumar, Nidhi Pareek
Fungal Biology Reviews.2022; 41: 52. CrossRef - Transcriptome analysis of genes associated with autolysis of Coprinus comatus
Hong-Bo Guo, Zhi-Fei Zhang, Jia-Qing Wang, Si-Yu Wang, Ji-Kang Yang, Xi-Yao Xing, Xiao-Jian Qi, Xiao-Dan Yu
Scientific Reports.2022;[Epub] CrossRef - Production of polyclonal antibody against human Neuritin and its application of immunodetection
Na Wang, Yu Wei, Wen Zhang, Xingyi Li, Jingling Zhu, Liya Shan, Chunyan Liu, Wumei Yuan, Jin Huang
Preparative Biochemistry and Biotechnology.2019; 49(3): 209. CrossRef - Identification and characterization of a marine-derived chitinolytic fungus, Acremonium sp. YS2-2
Dawoon Chung, Kyunghwa Baek, Seung Seob Bae, Jaejoon Jung
Journal of Microbiology.2019; 57(5): 372. CrossRef - Lentinula edodes Genome Survey and Postharvest Transcriptome Analysis
Yuichi Sakamoto, Keiko Nakade, Shiho Sato, Kentaro Yoshida, Kazuhiro Miyazaki, Satoshi Natsume, Naotake Konno, Daniel Cullen
Applied and Environmental Microbiology.2017;[Epub] CrossRef - きのこ類のキチン分解酵素と形態形成への寄与
Yuichi SAKAMOTO, Naotake KONNO
KAGAKU TO SEIBUTSU.2017; 55(10): 663. CrossRef - Heterologous Expression and Characterization of a Novel Chitinase (ChiEn1) from Coprinopsis cinerea and its Synergism in the Degradation of Chitin
Xin Niu, Jiang-Sheng Zhou, Yan-Xin Wang, Cui-Cui Liu, Zhong-Hua Liu, Sheng Yuan
Journal of Agricultural and Food Chemistry.2017; 65(32): 6943. CrossRef - Purification, characterization and physiological significance of a chitinase from the pilei ofCoprinopsis cinereafruiting bodies
Yajun Zhou, Liqin Kang, Xin Niu, Jun Wang, Zhonghua Liu, Sheng Yuan, Paolina Garbeva
FEMS Microbiology Letters.2016; 363(12): fnw120. CrossRef - Inhibition of cell wall synthesis in Cryptococcus neoformans and decrease of skin allergy induced with Alternaria alternata in mouse model by a chitinase from an inky cap
Yuri Kang, Hyoung T. Choi
The Korean Journal of Microbiology.2016; 52(2): 226. CrossRef - The Modes of Action of ChiIII, a Chitinase from Mushroom Coprinopsis cinerea, Shift with Changes in the Length of GlcNAc Oligomers
Xin Niu, Cui-Cui Liu, Yuan-Jing Xiong, Ming-Mei Yang, Fei Ma, Zhong-Hua Liu, Sheng Yuan
Journal of Agricultural and Food Chemistry.2016; 64(37): 6958. CrossRef - Comparative Study of Nonautolytic Mutant and Wild-Type Strains of Coprinopsis cinerea Supports an Important Role of Glucanases in Fruiting Body Autolysis
Zhonghua Liu, Xin Niu, Jun Wang, Wenming Zhang, Mingmei Yang, Cuicui Liu, Yuanjing Xiong, Yan Zhao, Siyu Pei, Qin Qin, Yu Zhang, Yuan Yu, Sheng Yuan
Journal of Agricultural and Food Chemistry.2015; 63(43): 9609. CrossRef - Molecular cloning, characterization, and expression analysis of a β-N-acetylhexosaminidase (LeHex20B) from the shiitake mushroom, Lentinula edodes
Naotake Konno, Ayumi Obara, Yuichi Sakamoto
Journal of Wood Science.2015; 61(2): 178. CrossRef - Novel Protease-Resistant Exochitinase (Echi47) from Pig Fecal Environment DNA with Application Potentials in the Food and Feed Industries
Yuchun Liu, Qiaojuan Yan, Shaoqing Yang, Zhengqiang Jiang
Journal of Agricultural and Food Chemistry.2015; 63(27): 6262. CrossRef - Expression and purification of recombinant human neuritin from Pichia pastoris and a partial analysis of its neurobiological activity in vitro
Yunhua Zhang, Shujun Zhang, Lingling Xian, Juan Tang, Jingling Zhu, Lijuan Cui, Shanman Li, Lei Yang, Jin Huang
Applied Microbiology and Biotechnology.2015; 99(19): 8035. CrossRef - Antifungal chitinase against human pathogenic yeasts from Coprinellus congregatus
Yeeun Yoo, Hyoung T. Choi
Journal of Microbiology.2014; 52(5): 441. CrossRef - Biochemical Characterization of Heterologously Expressed Chitinase 1 (Chi1) from an Inky Cap, Coprinellus congregatus
Yeeun Yoo, Hyoung T. Choi
The Korean Journal of Microbiology.2013; 49(4): 309. CrossRef