Evaluation of Fusarium Head Blight in Barley Infected by Fusarium graminearum

Woo-Ri Kang; Duk-Ju Hwang; Shin-Chul Bae; Theresa Lee; Soonok Kim; Il-Pyung Ahn

doi:10.1007/s12275-013-3338-8

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Research Support, Non-U.S. Gov't
Evaluation of Fusarium Head Blight in Barley Infected by Fusarium graminearum: Woo-Ri Kang ¹, Duk-Ju Hwang ¹, Shin-Chul Bae ¹, Theresa Lee ¹, Soonok Kim ², Il-Pyung Ahn ¹; Journal of Microbiology 2013;51(4):540-543.
DOI: https://doi.org/10.1007/s12275-013-3338-8
Published online: August 30, 2013

¹National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea, ²Wildlife Genetic Resources Centre, National Institute of Biological Resources, Incheon 404-708, Republic of Korea

Corresponding author: Il-Pyung Ahn , Tel: +82-31-299-1724,

Received: 24 June 2013 • Accepted: 29 July 2013

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Abstract

Fusarium head blight, which is primarily caused by Fusarium graminearum, is a devastating disease in the barley field. A real-time PCR protocol was developed to evaluate the growth of this pathogen in the host plant tissues. All four strains harbored the gene encoding ATP-BINDING CASSETTE TRANSPORTER (FgABC; FGSG_00541) as a single copy within their genomes. Our Southern blot result was identical with the genomic data for F. graminearum strain PH-1. Based on the crossing point (CP) values obtained in our TaqMan real-time PCR analysis, two standard curves describing the relationship among the CP value, FgABC copy number, and amount of fungal DNA were constructed. Chronological enumeration of fungal growth was coincided with the symptom development.

Keywords: Gibberella zeae;TaqMan real-time PCR

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Evaluation of Fusarium Head Blight in Barley Infected by Fusarium graminearum

J. Microbiol. 2013;51(4):540-543. Published online August 30, 2013

DOI: https://doi.org/10.1007/s12275-013-3338-8

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