Analysis of Bacterial Diversity in Sponges Collected off Chujado, an Island in Korea, Using Barcoded 454 Pyrosequencing: Analysis of a Distinctive Sponge Group Containing Chloroflexi

In-Hye Jeong; Kyoung-Ho Kim; Jin-Sook Park

doi:10.1007/s12275-013-3426-9

Articles

Page Path: HOME > J. Microbiol > Volume 51(5); 2013 > Article

Research Support, Non-U.S. Gov't
Analysis of Bacterial Diversity in Sponges Collected off Chujado, an Island in Korea, Using Barcoded 454 Pyrosequencing: Analysis of a Distinctive Sponge Group Containing Chloroflexi: In-Hye Jeong ¹, Kyoung-Ho Kim ², Jin-Sook Park ¹; Journal of Microbiology 2013;51(5):570-577.
DOI: https://doi.org/10.1007/s12275-013-3426-9
Published online: October 31, 2013

¹Department of Biological Science and Biotechnology, Hannam University, Daejeon 305-811, Republic of Korea, ²Department of Microbiology, Pukyong National University, Pusan 608-737, Republic of Korea¹Department of Biological Science and Biotechnology, Hannam University, Daejeon 305-811, Republic of Korea, ²Department of Microbiology, Pukyong National University, Pusan 608-737, Republic of Korea

Corresponding author: Jin-Sook Park , Tel: +82-42-629-8771,

Received: 9 August 2013 • Accepted: 4 October 2013

prev next

74 Views
0 Download
12 Crossref
14 Scopus

Full Article

Abstract

The bacterial diversity of 14 sponges belonging to 5 different orders that were collected around Chuja Island, Korea was investigated using barcoded 454 pyrosequencing. The sponges contained many unidentified bacterial groups (e.g. more than half of the taxa at the family level) that were known only in environmental sequences and obtained from culture-independent methods. Five of the sponges were clustered into one notable group (CF group), which was distinguished from the other sponges in accordance with bacterial composition (the other sponges may be separated into more groups but clustering is not clear). The CF group contained high amounts of Chloroflexi (25.0–47.7%) and moderate amounts of Gemmatimonadetes (2.3–7.0%), AncK6 (0.6–2.2%), PAUC34f (0.8–6.0%), Acidobacteria (3.7–9.6%), and SBR1093 (1.8–5.6%) exclusively or almost exclusively to this group. Sponges in the CF group also showed higher diversity (e.g. Shannon index) than the other sponges and contained group-specific taxonomic lineages (e.g. class or family level) from group-specific phyla and even from the Proteobacteria and Actinobacteria, which were detected in all sponges at the phylum level. The CF group may be one of the most distinctive groups in sponges in terms of bacterial diversity.

Keywords: sponge;bacterial diversity;454 pyrosequencing;Chujado;Chloroflexi group

Figure & Data

References

Citations

Citations to this article as recorded by

The effect of environmental and anthropogenic factors on the microbiome of the sponge, Halichondria panicea, at three coastal sites with different bathing water quality in North east England
Sakinah Al-Haddad, Gary S. Caldwell, Anthony S. Clare
Aquatic Sciences.2025;[Epub] CrossRef
Diversity and functional roles of the symbiotic microbiome associated to marine sponges off Karah Island, Terengganu, Malaysia
Yuki Hayami, Logajothiswaran Ambalavanan, Sandra Catherine Zainathan, Muhd Danish-Daniel, Noor Emilia Sharifah, Shumpei Iehata
Regional Studies in Marine Science.2023; 62: 102967. CrossRef
Pyrosequencing reveals sponge specific bacterial communities in marine sponges of Red Sea, Saudi Arabia
Fehmida Bibi, Sana Akhtar Alvi, Abdulmohsin Al-Sofyani, Muhammad Imran Naseer, Muhammad Yasir, Esam Ibraheem Azhar
Saudi Journal of Biological Sciences.2020; 27(1): 67. CrossRef
First Record of Microbiomes of Sponges Collected From the Persian Gulf, Using Tag Pyrosequencing
Akram Najafi, Maryam Moradinasab, Iraj Nabipour
Frontiers in Microbiology.2018;[Epub] CrossRef
Prokaryote composition and predicted metagenomic content of two Cinachyrella Morphospecies and water from West Papuan Marine Lakes
Daniel F R Cleary, Ana R M Polónia, Nicole J de Voogd
FEMS Microbiology Ecology.2018;[Epub] CrossRef
Metabolic Roles of Uncultivated Bacterioplankton Lineages in the Northern Gulf of Mexico “Dead Zone”
J. Cameron Thrash, Kiley W. Seitz, Brett J. Baker, Ben Temperton, Lauren E. Gillies, Nancy N. Rabalais, Bernard Henrissat, Olivia U. Mason, Mary Ann Moran
mBio.2017;[Epub] CrossRef
Intestinal microecology associated with fluoride resistance capability of the silkworm (Bombyx mori L.)
Guan-Nan Li, Xue-Juan Xia, Wen-Chao Tang, Yong Zhu
Applied Microbiology and Biotechnology.2016; 100(15): 6715. CrossRef
In four shallow and mesophotic tropical reef sponges from Guam the microbial community largely depends on host identity
Georg Steinert, Michael W. Taylor, Peter Deines, Rachel L. Simister, Nicole J. de Voogd, Michael Hoggard, Peter J. Schupp
PeerJ.2016; 4: e1936. CrossRef
Effect of long-term different fertilization on bacterial community structures and diversity in citrus orchard soil of volcanic ash
Jae Ho Joa, Hang Yeon Weon, Hae Nam Hyun, Young Chull Jeun, Sang Wook Koh
Journal of Microbiology.2014; 52(12): 995. CrossRef
Two distinct microbial communities revealed in the sponge Cinachyrella
Marie L. Cuvelier, Emily Blake, Rebecca Mulheron, Peter J. McCarthy, Patricia Blackwelder, Rebecca L. Vega Thurber, Jose V. Lopez
Frontiers in Microbiology.2014;[Epub] CrossRef
Analysis of bacterial diversity in sponges collected from chuuk and kosrae islands in micronesia
In-Hye Jeong, Kyoung-Ho Kim, Hyi-Seung Lee, Jin-Sook Park
Journal of Microbiology.2014; 52(1): 20. CrossRef
Bacterial Diversity of the South Pacific Sponge, Dactylospongia metachromia Based on DGGE Fingerprinting
In-Hye Jeong, Jin-Sook Park
The Korean Journal of Microbiology.2013; 49(4): 377. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Analysis of Bacterial Diversity in Sponges Collected off Chujado, an Island in Korea, Using Barcoded 454 Pyrosequencing: Analysis of a Distinctive Sponge Group Containing Chloroflexi

J. Microbiol. 2013;51(5):570-577. Published online October 31, 2013

DOI: https://doi.org/10.1007/s12275-013-3426-9

Related articles