Bacterial Diversity and Composition of an Alkaline Uranium Mine Tailings-Water Interface

Nurul H. Khan; Viorica F. Bondici; Prabhakara G. Medihala; John R. Lawrence; Gideon M. Wolfaardt; Jeff Warner; Darren R. Korber

doi:10.1007/s12275-013-3075-z

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Research Support, Non-U.S. Gov't
Bacterial Diversity and Composition of an Alkaline Uranium Mine Tailings-Water Interface: Nurul H. Khan ¹, Viorica F. Bondici ¹, Prabhakara G. Medihala ¹, John R. Lawrence ², Gideon M. Wolfaardt ³, Jeff Warner ⁴, Darren R. Korber ¹; Journal of Microbiology 2013;51(5):558-569.
DOI: https://doi.org/10.1007/s12275-013-3075-z
Published online: September 14, 2013

¹Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK, Canada, ²Environment Canada, Saskatoon, SK, Canada, ³Department of Chemistry and Biology, Ryerson University, Toronto, ON, Canada, ⁴Cameco Corporation, Saskatoon, SK, Canada¹Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK, Canada, ²Environment Canada, Saskatoon, SK, Canada, ³Department of Chemistry and Biology, Ryerson University, Toronto, ON, Canada, ⁴Cameco Corporation, Saskatoon, SK, Canada

Corresponding author: Darren R. Korber , Tel: +1-306-966-7786,

Received: 31 January 2013 • Accepted: 19 April 2013

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Abstract

The microbial diversity and biogeochemical potential associated with a northern Saskatchewan uranium mine watertailings interface was examined using culture-dependent and -independent techniques. Morphologically-distinct colonies from uranium mine water-tailings and a reference lake (MC) obtained using selective and non-selective media were selected for 16S rRNA gene sequencing and identification, revealing that culturable organisms from the uranium tailings interface were dominated by Firmicutes and Betaproteobacteria; whereas, MC organisms mainly consisted of Bacteroidetes and Gammaproteobacteria. Ion Torrent (IT) 16S rRNA metagenomic analysis carried out on extracted DNA from tailings and MC interfaces demonstrated the dominance of Firmicutes in both of the systems. Overall, the tailings-water interface environment harbored a distinct bacterial community relative to the MC, reflective of the ambient conditions (i.e., total dissolved solids, pH, salinity, conductivity, heavy metals) dominating the uranium tailings system. Significant correlations among the physicochemical data and the major bacterial groups present in the tailings and MC were also observed. Presence of sulfate reducing bacteria demonstrated by culture-dependent analyses and the dominance of Desulfosporosinus spp. indicated by Ion Torrent analyses within the tailings-water interface suggests the existence of anaerobic microenvironments along with the potential for reductive metabolic processes.

Keywords: bacterial diversity;uranium mine tailing;sediment water interface;sulphate reducing bacteria;Ion Torrent analyses

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Bacterial Diversity and Composition of an Alkaline Uranium Mine Tailings-Water Interface

J. Microbiol. 2013;51(5):558-569. Published online September 14, 2013

DOI: https://doi.org/10.1007/s12275-013-3075-z

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