NOTE] Next-Generation Sequencing-Based Transcriptome Analysis of L-Lysine-Producing Corynebacterium glutamicum ATCC 21300 Strain

Hong-Il Kim; Jae-Young Nam; Jae-Yong Cho; Chang-Soo Lee; Young-Jin Park

doi:10.1007/s12275-013-3236-0

Articles

Page Path: HOME > J. Microbiol > Volume 51(6); 2013 > Article

Research Support, Non-U.S. Gov't
NOTE] Next-Generation Sequencing-Based Transcriptome Analysis of L-Lysine-Producing Corynebacterium glutamicum ATCC 21300 Strain: Hong-Il Kim ¹, Jae-Young Nam ¹, Jae-Yong Cho ², Chang-Soo Lee ¹, Young-Jin Park ¹; Journal of Microbiology 2013;51(6):877-880.
DOI: https://doi.org/10.1007/s12275-013-3236-0
Published online: December 19, 2013

¹Department of Biomedical Chemistry, Konkuk University, Chung-Ju 380-701, Republic of Korea, ²Department of Pharmaceutical Engineering, Sangji University, Wonju 220-702, Republic of Korea¹Department of Biomedical Chemistry, Konkuk University, Chung-Ju 380-701, Republic of Korea, ²Department of Pharmaceutical Engineering, Sangji University, Wonju 220-702, Republic of Korea

Corresponding author: Young-Jin Park , Tel: +82-43-840-3572,

Received: 23 April 2013 • Accepted: 15 July 2013

prev next

33 Views
0 Download
4 Crossref
6 Scopus

Full Article

Abstract

In the present study, 151 genes showed a significant change in their expression levels in Corynebacterium glutamicum ATCC 21300 compared with those of C. glutamicum ATCC 13032. Of these 151 genes, 56 genes (2%) were up-regulated and 95 genes (3%) were down-regulated. RNA sequencing analysis also revealed that 11 genes, involved in the L-lysine biosynthetic pathway of C. glutamicum, were up- or downregulated compared with those of C. glutamicum ATCC 13032. Of the 151 genes, 10 genes were identified to have mutations including SNP (9 genes) and InDel (1 gene). This information will be useful for genome breeding of C. glutamicum to develop an industrial amino acid-producing strain with minimal mutation.

Keywords: transcriptome;mutation;genome;Corynebacterium glutamicum;RT-PCR

Figure & Data

References

Citations

Citations to this article as recorded by

Review of the Proteomics and Metabolic Properties of Corynebacterium glutamicum
Juhwan Park, Sooa Lim
Microorganisms.2024; 12(8): 1681. CrossRef
Transcriptome analysis of l-leucine-producing Corynebacterium glutamicum under the addition of trimethylglycine
Jian Wang, Xuesong Wang, Qing Liang, Deheng Li, Dawei Li, Qunqun Guo
Amino Acids.2022; 54(2): 229. CrossRef
RETRACTED ARTICLE: Comparative analysis of the Corynebacterium glutamicum transcriptome in response to changes in dissolved oxygen levels
Xiuxia Liu, Sun Yang, Fen Wang, Xiaofeng Dai, Yankun Yang, Zhonghu Bai
Journal of Industrial Microbiology and Biotechnology.2017; 44(2): 181. CrossRef
Transcriptome and Gene Ontology (GO) Enrichment Analysis Reveals Genes Involved in Biotin Metabolism That Affect l-Lysine Production in Corynebacterium glutamicum
Hong-Il Kim, Jong-Hyeon Kim, Young-Jin Park
International Journal of Molecular Sciences.2016; 17(3): 353. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

NOTE] Next-Generation Sequencing-Based Transcriptome Analysis of L-Lysine-Producing Corynebacterium glutamicum ATCC 21300 Strain

J. Microbiol. 2013;51(6):877-880. Published online December 19, 2013

DOI: https://doi.org/10.1007/s12275-013-3236-0

Related articles