NOTE] Next-Generation Sequencing-Based Transcriptome Analysis of L-Lysine-Producing Corynebacterium glutamicum ATCC 21300 Strain

Hong-Il Kim; Jae-Young Nam; Jae-Yong Cho; Chang-Soo Lee; Young-Jin Park

doi:10.1007/s12275-013-3236-0

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Research Support, Non-U.S. Gov't
NOTE] Next-Generation Sequencing-Based Transcriptome Analysis of L-Lysine-Producing Corynebacterium glutamicum ATCC 21300 Strain: Hong-Il Kim ¹, Jae-Young Nam ¹, Jae-Yong Cho ², Chang-Soo Lee ¹, Young-Jin Park ¹; Journal of Microbiology 2013;51(6):877-880.
DOI: https://doi.org/10.1007/s12275-013-3236-0
Published online: December 19, 2013

¹Department of Biomedical Chemistry, Konkuk University, Chung-Ju 380-701, Republic of Korea, ²Department of Pharmaceutical Engineering, Sangji University, Wonju 220-702, Republic of Korea¹Department of Biomedical Chemistry, Konkuk University, Chung-Ju 380-701, Republic of Korea, ²Department of Pharmaceutical Engineering, Sangji University, Wonju 220-702, Republic of Korea

Corresponding author: Young-Jin Park , Tel: +82-43-840-3572,

Received: 23 April 2013 • Accepted: 15 July 2013

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Abstract

In the present study, 151 genes showed a significant change in their expression levels in Corynebacterium glutamicum ATCC 21300 compared with those of C. glutamicum ATCC 13032. Of these 151 genes, 56 genes (2%) were up-regulated and 95 genes (3%) were down-regulated. RNA sequencing analysis also revealed that 11 genes, involved in the L-lysine biosynthetic pathway of C. glutamicum, were up- or downregulated compared with those of C. glutamicum ATCC 13032. Of the 151 genes, 10 genes were identified to have mutations including SNP (9 genes) and InDel (1 gene). This information will be useful for genome breeding of C. glutamicum to develop an industrial amino acid-producing strain with minimal mutation.

Keywords: transcriptome;mutation;genome;Corynebacterium glutamicum;RT-PCR

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NOTE] Next-Generation Sequencing-Based Transcriptome Analysis of L-Lysine-Producing Corynebacterium glutamicum ATCC 21300 Strain

J. Microbiol. 2013;51(6):877-880. Published online December 19, 2013

DOI: https://doi.org/10.1007/s12275-013-3236-0

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