A Putative APSES Transcription Factor Is Necessary for Normal Growth and Development of Aspergillus nidulans

Ji-Yeon Lee; Lee-Han Kim; Ha-Eun Kim; Jae-Sin Park; Kap-Hoon Han; Dong-Min Han

doi:10.1007/s12275-013-3100-2

Articles

Page Path: HOME > J. Microbiol > Volume 51(6); 2013 > Article

Research Support, Non-U.S. Gov't
A Putative APSES Transcription Factor Is Necessary for Normal Growth and Development of Aspergillus nidulans: Ji-Yeon Lee ¹, Lee-Han Kim ¹, Ha-Eun Kim ¹, Jae-Sin Park ¹, Kap-Hoon Han ², Dong-Min Han ¹; Journal of Microbiology 2013;51(6):800-806.
DOI: https://doi.org/10.1007/s12275-013-3100-2
Published online: December 19, 2013

¹Division of Life Science, Wonkwang University, Iksan 570-749, Republic of Korea, ²Department of Pharmaceutical Engineering, Woosuk University, Wanju 565-901, Republic of Korea¹Division of Life Science, Wonkwang University, Iksan 570-749, Republic of Korea, ²Department of Pharmaceutical Engineering, Woosuk University, Wanju 565-901, Republic of Korea

Corresponding author: Dong-Min Han , Tel: +82-63-850-6220,

Received: 15 February 2013 • Accepted: 11 June 2013

prev next

49 Views
0 Download
13 Crossref
11 Scopus

Full Article

Abstract

The nsdD gene encoding a GATA type transcription factor positively controls sexual development in Aspergillus nidulans. According to microarray data, 20 genes that were upregulated by deleting nsdD during various life cycle stages were randomly selected and deleted for functional analysis. None of the mutants showed apparent changes in growth or development compared with those of the wild-type except the AN3154 gene that encodes a putative APSES transcription factor and is an ortholog of Saccharomyces cerevisiae swi4. Deleting AN3154 resulted in retarded growth and development, and the gene was named rgdA (retared growth and development). The rgdA deletion mutant developed a reduced number of conidia even under favorable conditions for asexual development. The retarded growth and development was partially suppressed by the veA1 mutation. The conidial heads of the mutant aborted, showing reduced and irregular shaped phialides. Fruiting body development was delayed compared with that in the wild-type. The mutant did not respond to various nutritional or environmental factors that affected the development patterns. The rgdA gene was expressed at low levels throughout the life cycle and was not significantly affected by several regulators of sexual and asexual development such as nsdD, veA, stuA, or brlA. However, the rgdA gene affected brlA and abaA expression, which function as key regulators of asexual sporulation, suggesting that rgdA functions upstream of those genes.

Keywords: Aspergillus nidulans;APSES transcription factor;rgdA;asexual development;VeA

Figure & Data

References

Citations

Citations to this article as recorded by

Putative APSES family transcription factor mbp1 plays an essential role in regulating cell wall synthesis in the agaricomycete Pleurotus ostreatus
Hayase Kojima, Moriyuki Kawauchi, Yuitsu Otsuka, Kim Schiphof, Kenya Tsuji, Akira Yoshimi, Chihiro Tanaka, Shigekazu Yano, Takehito Nakazawa, Yoichi Honda
Fungal Genetics and Biology.2024; 175: 103936. CrossRef
Regulators of the Asexual Life Cycle of Aspergillus nidulans
Ye-Eun Son, Jae-Hyuk Yu, Hee-Soo Park
Cells.2023; 12(11): 1544. CrossRef
Characterization of the mbsA Gene Encoding a Putative APSES Transcription Factor in Aspergillus fumigatus
Yong-Ho Choi, Sang-Cheol Jun, Min-Woo Lee, Jae-Hyuk Yu, Kwang-Soo Shin
International Journal of Molecular Sciences.2021; 22(7): 3777. CrossRef
The Putative APSES Transcription Factor RgdA Governs Growth, Development, Toxigenesis, and Virulence in Aspergillus fumigatus
Sang-Cheol Jun, Yong-Ho Choi, Min-Woo Lee, Jae-Hyuk Yu, Kwang-Soo Shin, Aaron P. Mitchell
mSphere.2020;[Epub] CrossRef
Analogous and Diverse Functions of APSES-Type Transcription Factors in the Morphogenesis of the Entomopathogenic Fungus Metarhizium rileyi
Caiyan Xin, Jinping Zhang, Siji Nian, Guangxi Wang, Zhongkang Wang, Zhangyong Song, Guangwei Ren, Ning-Yi Zhou
Applied and Environmental Microbiology.2020;[Epub] CrossRef
Distribution, evolution and expression ofGATA-TFsprovide new insights into their functions in light response and fruiting body development ofTolypocladium guangdongense
Chenghua Zhang, Gangzheng Wang, Wangqiu Deng, Taihui Li
PeerJ.2020; 8: e9784. CrossRef
Characterization of the APSES-family transcriptional regulators of Histoplasma capsulatum
Larissa V G Longo, Stephanie C Ray, Rosana Puccia, Chad A Rappleye
FEMS Yeast Research.2018;[Epub] CrossRef
Essential APSES Transcription Factors for Mycotoxin Synthesis, Fungal Development, and Pathogenicity in Aspergillus flavus
Guangshan Yao, Feng Zhang, Xinyi Nie, Xiuna Wang, Jun Yuan, Zhenhong Zhuang, Shihua Wang
Frontiers in Microbiology.2017;[Epub] CrossRef
Characterizing the nuclear proteome of Paracoccidioides spp.
Lucas Nojosa Oliveira, Luciana Casaletti, Sônia Nair Báo, Clayton Luiz Borges, Patrícia de Sousa Lima, Célia Maria de Almeida Soares
Fungal Biology.2016; 120(10): 1209. CrossRef
Isolation and Characterization of Two Methyltransferase Genes, AfuvipB and AfuvipC in Aspergillus fumigatus
Mohammed A. Abdo Elgabbar, Kap-Hoon Han
The Korean Journal of Mycology.2015; 43(1): 33. CrossRef
Depletion of ε-COP in the COPI Vesicular Coat Reduces Cleistothecium Production inAspergillus nidulans
Eun-Hye Kang, Eun-Jung Song, Jun Ho Kook, Hwan-Hee Lee, Bo-Ri Jeong, Hee-Moon Park
Mycobiology.2015; 43(1): 31. CrossRef
FgFlbD regulates hyphal differentiation required for sexual and asexual reproduction in the ascomycete fungus Fusarium graminearum
Hokyoung Son, Myung-Gu Kim, Suhn-Kee Chae, Yin-Won Lee
Journal of Microbiology.2014; 52(11): 930. CrossRef
Transcriptional regulation of fksA, a β-1,3-glucan synthase gene, by the APSES protein StuA during Aspergillus nidulans development
Bum-Chan Park, Yun-Hee Park, Soohyun Yi, Yu Kyung Choi, Eun-Hye Kang, Hee-Moon Park
Journal of Microbiology.2014; 52(11): 940. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

A Putative APSES Transcription Factor Is Necessary for Normal Growth and Development of Aspergillus nidulans

J. Microbiol. 2013;51(6):800-806. Published online December 19, 2013

DOI: https://doi.org/10.1007/s12275-013-3100-2

Related articles