Cloning and Functional Analysis of the Gβ Gene Mgb1 and the Gγ Gene Mgg1 in Monascus ruber

Li Li; Lu He; Yong Lai; Yanchun Shao; Fusheng Chen

doi:10.1007/s12275-014-3072-x

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Research Support, Non-U.S. Gov't
Cloning and Functional Analysis of the Gβ Gene Mgb1 and the Gγ Gene Mgg1 in Monascus ruber: Li Li ^1,2, Lu He ¹, Yong Lai ¹, Yanchun Shao ¹, Fusheng Chen ^1,3; Journal of Microbiology 2014;52(1):35-43.
DOI: https://doi.org/10.1007/s12275-014-3072-x
Published online: January 4, 2014

¹College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, P. R. China, ²College of Life Science, Yangtze University, Jingzhou, 434025, Hubei Province, P. R. China, ³Key Laboratory of Environment Correlative Dietology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, Hubei Province, P. R. China¹College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, P. R. China, ²College of Life Science, Yangtze University, Jingzhou, 434025, Hubei Province, P. R. China, ³Key Laboratory of Environment Correlative Dietology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, Hubei Province, P. R. China

Corresponding author: Fusheng Chen , Tel: +86-27-87282927,

Received: 31 January 2013 • Revised: 18 July 2013 • Accepted: 31 July 2013

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Abstract

The ascomycetous fungus Monascus ruber is one of the most well-known species widely used to produce Monascus-fermentation products for natural food colorants and medicine. Our previous research on the Gα subunit Mga1 and the regulator of G protein signaling MrflbA indicated that heterotrimeric G protein signaling pathways were involved in aspects of growth, sporulation and secondary metabolite production in M. ruber. To better understand the G protein signaling pathways in this fungus, a Gβ subunit gene (Mgb1) and a Gγ subunit gene (Mgg1) were cloned and investigated in the current study. The predicted Mgb1 protein consisted of 353 amino acids and Mgg1 consisted of 94 amino acids, sharing marked similarity with Aspergillus Gβ and Gγ subunits, respectively. Targeted deletion (Δ) of Mgb1 or Mgg1
result
ed in phenotypic alterations similar to those resulting from ΔMga1, i.e., restricted vegetative growth, lowered asexual sporulation, impaired cleistothecial formation, and enhanced citrinin and pigment production. Moreover, deletion of Mgg1 suppressed the defects in asexual development and in biosynthesis of citrinin and pigment caused by the absence of MrflbA function. These results provide evidence that Mgb1 and Mgg1 form a functional Gβγ dimer and the dimer interacts with Mga1 to mediate signaling pathways, which are negatively controlled by MrflbA, for growth, reproduction and citrinin and pigment biosynthesis in M. ruber.

Keywords: Monascus ruber;G-protein beta gamma;growth;reproduction;citrinin;pigment

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Cloning and Functional Analysis of the Gβ Gene Mgb1 and the Gγ Gene Mgg1 in Monascus ruber

J. Microbiol. 2014;52(1):35-43. Published online January 4, 2014

DOI: https://doi.org/10.1007/s12275-014-3072-x

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