Molecular Characterization of the Alpha Subunit of Multicomponent Phenol Hydroxylase from 4-Chlorophenol-Degrading Pseudomonas sp. Strain PT3

Wael S. El-Sayed; Mohamed K. Ibrahim; Salama A. Ouf

doi:10.1007/s12275-014-3250-x

Articles

Page Path: HOME > J. Microbiol > Volume 52(1); 2014 > Article

Research Support, Non-U.S. Gov't
Molecular Characterization of the Alpha Subunit of Multicomponent Phenol Hydroxylase from 4-Chlorophenol-Degrading Pseudomonas sp. Strain PT3: Wael S. El-Sayed ^1,2, Mohamed K. Ibrahim ², Salama A. Ouf ^1,3; Journal of Microbiology 2014;52(1):13-19.
DOI: https://doi.org/10.1007/s12275-014-3250-x
Published online: January 4, 2014

¹Biology Department, Faculty of Science, Taibah University, 344, Almadinah Almunwarrah, KSA, ²Microbiology Department, Faculty of Science, Ain Shams University, 11566, Cairo, Egypt, ³Botany Department, Faculty of Science, Cairo University, Giza 12613, Egypt¹Biology Department, Faculty of Science, Taibah University, 344, Almadinah Almunwarrah, KSA, ²Microbiology Department, Faculty of Science, Ain Shams University, 11566, Cairo, Egypt, ³Botany Department, Faculty of Science, Cairo University, Giza 12613, Egypt

Received: 6 May 2013 • Revised: 6 August 2013 • Accepted: 13 August 2013

prev next

49 Views
0 Download
6 Crossref
8 Scopus

Full Article

Abstract

Multicomponent phenol hydroxylases (mPHs) are diiron enzymes that use molecular oxygen to hydroxylate a variety of phenolic compounds. The DNA sequence of the alpha subunit (large subunit) of mPH from 4-chlorophenol (4-CP)- degrading bacterial strain PT3 was determined. Strain PT3 was isolated from oil-contaminated soil samples adjacent to automobile workshops and oil stations after enrichment and establishment of a chlorophenol-degrading consortium. Strain PT3 was identified as a member of Pseudomonas sp. based on sequence analysis of the 16S rRNA gene fragment. The 4-CP catabolic pathway by strain PT3 was tentatively proposed to proceed via a meta-cleavage pathway after hydroxylation to the corresponding chlorocatechol. This hypothesis was supported by polymerase chain reaction (PCR) detection of the LmPH encoding sequence and UV/VIS spectrophotometric analysis of the culture filtrate showing accumulation of 5-chloro-2-hydroxymuconic semialdehyde (5-CHMS) with λmax 380. The detection of catabolic genes involved in 4-CP degradation by PCR showed the presence of both mPH and catechol 2,3-dioxygenase (C23DO). Nucleotide sequence analysis of the alpha subunit of mPH from strain PT3 revealed specific phylogenetic grouping to known mPH. The metal coordination encoding regions from strain PT3 were found to be conserved with those from the homologous dinuclear oxo-iron bacterial monooxygenases. Two DE(D)XRH motifs was detected in LmPH of strain PT3 within an approximate 100 amino acid interval, a typical arrangement characteristic of most known PHs.

Keywords: 4-chlorophenol;biodegradation;phenol hydroxylase;catalytic domain;Pseudomonas

Figure & Data

References

Citations

Citations to this article as recorded by

An investigation of soil and groundwater metagenomes for genes encoding soluble and particulate methane monooxygenase, toluene-4-monoxygenase, propane monooxygenase and phenol hydroxylase
Alison M. Cupples, Hongyu Dang, Katy Foss, Anat Bernstein, Jean-Rene Thelusmond
Archives of Microbiology.2024;[Epub] CrossRef
Functionality, characterization and DEGs contribution by engineering isolate Pseudomonas P1 to elucidate the regulation mechanisms of p-chlorophenol-4-Chloroaniline bioremediation
Shuo Sun, Yaru Wang, Shuiquan Chen, Zhenhai Li, Chaocheng Zhao, Qiyou Liu, Xiuxia Zhang
Chemical Engineering Journal.2023; 468: 143798. CrossRef
Challenges and opportunities for the biodegradation of chlorophenols: Aerobic, anaerobic and bioelectrochemical processes
Kaichao Yang, Yingxin Zhao, Min Ji, Zhiling Li, Siyuan Zhai, Xu Zhou, Qian Wang, Can Wang, Bin Liang
Water Research.2021; 193: 116862. CrossRef
Metabolic profile analysis and kinetics of p-cresol biodegradation by an indigenous Pseudomonas citronellolis NS1 isolated from coke oven wastewater
Namita Panigrahy, Manas Barik, Rajesh Kumar Sahoo, Naresh Kumar Sahoo
International Biodeterioration & Biodegradation.2020; 147: 104837. CrossRef
Phenol hydroxylase from Pseudomonas sp. KZNSA: Purification, characterization and prediction of three-dimensional structure
Boitumelo Setlhare, Ajit Kumar, Mduduzi P. Mokoena, Bala Pillay, Ademola O. Olaniran
International Journal of Biological Macromolecules.2020; 146: 1000. CrossRef
Characterization and biodegradation potential of an aniline-degrading strain of Pseudomonas JA1 at low temperature
Yu Jiang, Yu Shang, Jun Zhou, Kai Yang, Hongyu Wang
Desalination and Water Treatment.2016; 57(52): 25011. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Molecular Characterization of the Alpha Subunit of Multicomponent Phenol Hydroxylase from 4-Chlorophenol-Degrading Pseudomonas sp. Strain PT3

J. Microbiol. 2014;52(1):13-19. Published online January 4, 2014

DOI: https://doi.org/10.1007/s12275-014-3250-x

Related articles