Comparison of JEV Neutralization Assay Using Pseudotyped JEV with the Conventional Plaque-Reduction Neutralization Test

Hee-Jung Lee; Kyung-Il Min; Ki Hoon Park; Hyo Jung Choi; Min-Kyoung Kim; Chi-Young Ahn; Young-Jin Hong; Young Bong Kim

doi:10.1007/s12275-014-3529-y

Articles

Page Path: HOME > J. Microbiol > Volume 52(5); 2014 > Article

Validation Study
Comparison of JEV Neutralization Assay Using Pseudotyped JEV with the Conventional Plaque-Reduction Neutralization Test: Hee-Jung Lee ¹, Kyung-Il Min ², Ki Hoon Park ¹, Hyo Jung Choi ¹, Min-Kyoung Kim ¹, Chi-Young Ahn ³, Young-Jin Hong ⁴, Young Bong Kim ¹; Journal of Microbiology 2014;52(5):435-440
DOI: https://doi.org/10.1007/s12275-014-3529-y
Published online: March 7, 2014

¹Department of Bio-industrial technologies, Konkuk University, Seoul 143-701, Republic of Korea, ²Department of Biopharmaceuticals and Herbal Medicine Evaluation, National Institute of Food and Drug Safety Evaluation, Osong 363-951, Republic of Korea, ³Blood Products Team, National Institute of Food and Drug Safety Evaluation, Osong 363-951, Republic of Korea, ⁴Department of Pediatrics, College of medicine, Inha University, Incheon 402-751, Republic of Korea¹Department of Bio-industrial technologies, Konkuk University, Seoul 143-701, Republic of Korea, ²Department of Biopharmaceuticals and Herbal Medicine Evaluation, National Institute of Food and Drug Safety Evaluation, Osong 363-951, Republic of Korea, ³Blood Products Team, National Institute of Food and Drug Safety Evaluation, Osong 363-951, Republic of Korea, ⁴Department of Pediatrics, College of medicine, Inha University, Incheon 402-751, Republic of Korea

Corresponding author: Young Bong Kim , Tel: +82-2-450-4208,

Received: 10 October 2013 • Revised: 13 December 2013 • Accepted: 16 December 2013

prev next

2 Views
0 Download
0 Crossref
18 Scopus

Full Article

Abstract

We previously reported the development of a neutralization assay system for evaluating Japanese Encephalitis Virus (JEV) neutralizing antibody (NAb) using pseudotyped-JEV (JEV- PV). JEV-PV-based neutralization assay offers several advan-tages compared with the current standard plaque-reduc-tion neutralization test (PRNT), including simplicity, safety, and speed. To evaluate the suitability of the JEV-PV assay as new replacement neutralization assay, we compared its repeatability, reproducibility, specificity, and correlated its results with those obtained using the PRNT. These analyses showed a close correlation between the results obtained with the JEV-PV assay and the PRNT, using the 50% plaque re-duction method as a standard for measuring NAb titers to JEV. The validation results met all analytical acceptance criteria. These results suggest that the JEV-PV assay could serve as a safe and simple method for measuring NAb titer against JEV and could be used as an alternative approach for assaying the potency of JEV neutralization.

Keywords: Japanese encephalitis virus; neutralizing antibody; pseudovirus; PRNT

Figure & Data

References

Citations

Citations to this article as recorded by

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Comparison of JEV Neutralization Assay Using Pseudotyped JEV with the Conventional Plaque-Reduction Neutralization Test

J. Microbiol. 2014;52(5):435-440. Published online March 7, 2014

DOI: https://doi.org/10.1007/s12275-014-3529-y

Related articles