Note] Identification of High-Specificity H-NS Binding Site in LEE5 Promoter of Enteropathogenic Esherichia coli (EPEC)

Abhay Prasad Bhat; Minsang Shin; Hyon E. Choy

doi:10.1007/s12275-014-3562-x

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Note] Identification of High-Specificity H-NS Binding Site in LEE5 Promoter of Enteropathogenic Esherichia coli (EPEC): Abhay Prasad Bhat ^1,2, Minsang Shin ^1,2, Hyon E. Choy ^1,2; Journal of Microbiology 2014;52(7):626-629.
DOI: https://doi.org/10.1007/s12275-014-3562-x
Published online: March 7, 2014

¹Center for Host Defense against Enteropathogenic Bacteria Infection, Chonnam National University Medical School, Kwangju 501-746, Republic of Korea, ²Department of Microbiology, Chonnam National University Medical School, Kwangju 501-746, Republic of Korea¹Center for Host Defense against Enteropathogenic Bacteria Infection, Chonnam National University Medical School, Kwangju 501-746, Republic of Korea, ²Department of Microbiology, Chonnam National University Medical School, Kwangju 501-746, Republic of Korea

Corresponding author: Hyon E. Choy , Tel: +82-62-220-4137,

Received: 5 October 2013 • Revised: 29 October 2013 • Accepted: 30 October 2013

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Abstract

Histone-like nucleoid structuring protein (H-NS) is a small but abundant protein present in enteric bacteria and is involved in compaction of the DNA and regulation of the transcription. Recent reports have suggested that H-NS binds to a specific AT rich DNA sequence than to intrinsically curved DNA in sequence independent manner. We detected two high-specificity H-NS binding sites in LEE5 promoter of EPEC centered at -110 and -138, which were close to the proposed consensus H-NS binding motif. To identify H-NS binding sequence in LEE5 promoter, we took a random mutagenesis approach and found the mutations at around -138 were specifically defective in the regulation byH-NS. It was concluded that H-NS exertsmaximumrepression via the specific sequence at around -138 and ubsequently contacts α subunit of RNAP through oligomerization.

Keywords: LEE5;H-NS;nucleation site;consensus motif;transcriptional repression;gene regulation;transcription

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Note] Identification of High-Specificity H-NS Binding Site in LEE5 Promoter of Enteropathogenic Esherichia coli (EPEC)

J. Microbiol. 2014;52(7):626-629. Published online March 7, 2014

DOI: https://doi.org/10.1007/s12275-014-3562-x

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