Abstract
			
			Bovine viral diarrhea virus (BVDV) is an enveloped, positive-sense, single-stranded RNA virus that belongs to the genus Pestivirus (Flaviviridae). The signaling pathways and levels of signaling molecules are altered in Madin-Darby Bovine Kidney (MDBK) cells infected with BVDV. Autophagy is a conservative biological degradation pathway that mainly eliminates and degrades damaged or superfluous organelles and macromolecular complexes for intracellular recycling in eukaryotic cells. Autophagy can also be induced as an effective response to maintain cellular homeostasis in response to different stresses, such as nutrient or growth factor deprivation, hypoxia, reactive oxygen species exposure and pathogen infection. However, the effects of BVDV infection on autophagy inMDBK cells remain unclear. Therefore, we performed an analysis of autophagic activity after BVDV NADL infection using real-time PCR, electron microscopy, laser confocal microscopy, and Western blotting analysis. The results demonstrated that BVDV NADL infection increased autophagic activity and significantly elevated the expression levels of the autophagy-related genes Beclin1 and ATG14 inMDBK cells. However, the knockdown of Beclin1 and ATG14 by RNA interference (RNAi) did not affect BVDV NADL infection-related autophagic activity. These findings provided a novel perspective to elaborate the effects of viral infection on the host cells.			
						
						
					 
		
		
		 
		
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			Citations to this article as recorded by  

- Non-cytopathic bovine viral diarrhea virus (BVDV) inhibits innate immune responses via induction of mitophagy
 Zhijun Li, Ying Zhang, Bao Zhao, Qinghong Xue, Chunjiang Wang, Siyu Wan, Jingyu Wang, Xiwen Chen, Xuefeng Qi
 Veterinary Research.2024;[Epub]     CrossRef
- Immune evasion strategies of bovine viral diarrhea virus
 Feng Pang, Qinqin Long, Min Wei
 Frontiers in Cellular and Infection Microbiology.2023;[Epub]     CrossRef
- bta-miR-2904 inhibits bovine viral diarrhea virus replication by targeting viral-infection-induced autophagy via ATG13
 Ningning Yang, Nana Hu, Jiangwei Zhang, Jihai Yi, Zhen Wang, Yong Wang, Peng Wu, Chuangfu Chen
 Archives of Virology.2023;[Epub]     CrossRef
- In Vivo and In Vitro Antiviral Activity of Phlorizin Against Bovine Viral Diarrhea Virus
 Zecai Zhang, Jiang Huang, Zhicheng Zhao, Xueying Yuan, Chuang Li, Siyu Liu, Yueqi Cui, Yu Liu, Yulong Zhou, Zhanbo Zhu
 Journal of Agricultural and Food Chemistry.2022; 70(47): 14841.     CrossRef
- The Multi-Faceted Role of Autophagy During Animal Virus Infection
 Hui Jiang, Xianjin Kan, Chan Ding, Yingjie Sun
 Frontiers in Cellular and Infection Microbiology.2022;[Epub]     CrossRef
- DDIT3 Targets Innate Immunity via the DDIT3-OTUD1-MAVS Pathway To Promote Bovine Viral Diarrhea Virus Replication
 Song Wang, Peili Hou, Wei Pan, Wenqi He, Daniel Chang He, Hongmei Wang, Hongbin He, Rozanne M. Sandri-Goldin
 Journal of Virology.2021;[Epub]     CrossRef
- Fate of the germ cells in mammalian ovary: A review
 Pramod K. Yadav, Anumegha Gupta, Alka Sharma, Anil Kumar Yadav, Meenakshi Tiwari, Ashutosh N. Pandey, Shilpa Prasad, Tulsidas G. Shrivastav, Shail K. Chaube
 Journal of Reproductive Health and Medicine.2020; 3: 3.     CrossRef
- Stress proteins: the biological functions in virus infection, present and challenges for target-based antiviral drug development
 Qianya Wan, Dan Song, Huangcan Li, Ming-liang He
 Signal Transduction and Targeted Therapy.2020;[Epub]     CrossRef
- Glucocorticoid-induced autophagy and apoptosis in bone
 Tiantian Wang, Xiaonan Liu, Chengqi He
 Apoptosis.2020; 25(3-4): 157.     CrossRef
- Effects of Starvation on Antioxidant-Related Signaling Molecules, Oxidative Stress, and Autophagy in Juvenile Chinese Perch Skeletal Muscle
 Ping Wu, Aimin Wang, Jia Cheng, Lin Chen, Yaxiong Pan, Honghui Li, Qi Zhang, Jiaqi Zhang, Wuying Chu, Jianshe Zhang
 Marine Biotechnology.2020; 22(1): 81.     CrossRef
- Autophagy: A Promising Target for Age-related Osteoporosis
 Tiantian Wang, Hongchen  He, Shaxin Liu, Chengsen  Jia, Ziyan Fan, Can Zhong, Jiadan Yu, Honghong Liu, Chengqi He
 Current Drug Targets.2019; 20(3): 354.     CrossRef
- A Comprehensive Review of Autophagy and Its Various Roles in Infectious, Non-Infectious, and Lifestyle Diseases: Current Knowledge and Prospects for Disease Prevention, Novel Drug Design, and Therapy
 Rekha Khandia, Maryam Dadar, Ashok Munjal, Kuldeep Dhama, Kumaragurubaran Karthik, Ruchi Tiwari, Mohd. Iqbal Yatoo, Hafiz M.N. Iqbal, Karam Pal Singh, Sunil K. Joshi, Wanpen Chaicumpa
 Cells.2019; 8(7): 674.     CrossRef
- Bovine viral diarrhea virus non-structural protein NS4B induces autophagosomes in bovine kidney cells
 Yuto Suda, Shin Murakami, Taisuke Horimoto
 Archives of Virology.2019; 164(1): 255.     CrossRef
- Comprehensive analysis of circRNAs expression profiles in different periods of MDBK cells infected with bovine viral diarrhea virus
 Cunyuan Li, Xiaoyue Li, Xiaoxu Hou, Wei Ni, Mengdan Zhang, Huixiang Li, Yueren Xu, Wureli Hazi, Qiman Ma, Yunfeng Zhang, Dawei Wang, Shengwei Hu
 Research in Veterinary Science.2019; 125: 52.     CrossRef
- Germ cell depletion from mammalian ovary: possible involvement of apoptosis and autophagy
 Pramod K. Yadav, Meenakshi Tiwari, Anumegha Gupta, Alka Sharma, Shilpa Prasad, Ashutosh N. Pandey, Shail K. Chaube
 Journal of Biomedical Science.2018;[Epub]     CrossRef
- Autophagy as a target for glucocorticoid-induced osteoporosis therapy
 Gengyang Shen, Hui Ren, Qi Shang, Ting Qiu, Xiang Yu, Zhida Zhang, Jinjing Huang, Wenhua Zhao, Yuzhuo Zhang, De Liang, Xiaobing Jiang
 Cellular and Molecular Life Sciences.2018; 75(15): 2683.     CrossRef
- Analyses of long non-coding RNAs and mRNA profiling through RNA sequencing of MDBK cells at different stages of bovine viral diarrhea virus infection
 Qiman Ma, Liangyuan Li, Yan Tang, Qiang Fu, Sheng Liu, Shengwei Hu, Jun Qiao, Chuangfu Chen, Wei Ni
 Research in Veterinary Science.2017; 115: 508.     CrossRef
- Both cytopathic and non-cytopathic bovine viral diarrhea virus (BVDV) induced autophagy at a similar rate
 Mrigendra K.S. Rajput, Karim Abdelsalam, Mahmoud F. Darweesh, Lyle J Braun, Jason Kerkvliet, Adam D. Hoppe, Christopher C.L. Chase
 Veterinary Immunology and Immunopathology.2017; 193-194: 1.     CrossRef
- Roles of Autophagy in Ischemic Heart Diseases and the Modulatory Effects of Chinese Herbal Medicine
 Dawei Wang, Weiqing Yu, Yuntao Liu, Guofu Zhong, Zhen Zhao, Xia Yan, Qing Liu
 The American Journal of Chinese Medicine.2017; 45(07): 1401.     CrossRef
- Crosstalk between Autophagy and Apoptosis: Potential and Emerging Therapeutic Targets for Cardiac Diseases
 Meng Li, Ping Gao, Junping Zhang
 International Journal of Molecular Sciences.2016; 17(3): 332.     CrossRef
- Roles of p53 and ASF1A in the Reprogramming of Sheep Kidney Cells to Pluripotent Cells
 Huijun Shi, Qiang Fu, Guozhong Li, Yan Ren, Shengwei Hu, Wei Ni, Fei Guo, Mengting Shi, Luping Meng, Hui Zhang, Jun Qiao, Zhiru Guo, Chuangfu Chen
 Cellular Reprogramming.2015; 17(6): 441.     CrossRef
- Recent insights into the role of autophagy in the pathogenesis of rheumatoid arthritis
 Yujie Dai, Shaoxian Hu
 Rheumatology.2015; : kev337.     CrossRef
- Structures and Functions of Pestivirus Glycoproteins: Not Simply Surface Matters
 Fun-In Wang, Ming-Chung Deng, Yu-Liang Huang, Chia-Yi Chang
 Viruses.2015; 7(7): 3506.     CrossRef