Molecular Characterization of Atoxigenic Aspergillus flavus Isolates Collected in China

Dandan Wei; Lu Zhou; Jonathan Nimal Selvaraj; Chushu Zhang; Fuguo Xing; Yueju Zhao; Yan Wang; Yang Liu

doi:10.1007/s12275-014-3629-8

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Molecular Characterization of Atoxigenic Aspergillus flavus Isolates Collected in China: Dandan Wei , Lu Zhou , Jonathan Nimal Selvaraj , Chushu Zhang , Fuguo Xing , Yueju Zhao , Yan Wang , Yang Liu; Journal of Microbiology 2014;52(7):559-565.
DOI: https://doi.org/10.1007/s12275-014-3629-8
Published online: May 30, 2014

Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Processing, Ministry of Agriculture, Beijing 100193, P. R. ChinaInstitute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Processing, Ministry of Agriculture, Beijing 100193, P. R. China

Corresponding author: Yang Liu , Tel: +86-10-62815874,

Received: 6 December 2013 • Revised: 18 February 2014 • Accepted: 24 February 2014

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Abstract

Aspergillus flavus strains were isolated from peanut fields of Liaoning, Shandong, Hubei and Guangdong Provinces in China, and identified through phenotypic and molecular approaches. Of the 323 A. flavus strains isolated, 76 strains did not produce aflatoxins detectable by UPLC. The incidence of atoxigenic A. flavus strains decreased with increase in temperature and increased with increase in latitude in different geographical locations. Amplification of all the aflatoxin genes in the aflatoxin gene cluster in the atoxigenic isolates showed that there were 25 deletion patterns (A-Y), with 22 deletion patterns identified for the first time. Most of the atoxigenic A. flavus isolates with gene deletions (97%) had deletions in at least one of the four genes (aflT, nor-1, aflR, and hypB), indicating that these four genes could be targeted for rapid identification of atoxigenic strains. The atoxigenic isolates with gene deletions, especially the isolates with large deletions, are potential candidates for aflatoxin control.

Keywords: aflatoxins;Aspergillus flavus;atoxigenic isolate;deletion pattern;gene cluster;biological control

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Molecular Characterization of Atoxigenic Aspergillus flavus Isolates Collected in China

J. Microbiol. 2014;52(7):559-565. Published online May 30, 2014

DOI: https://doi.org/10.1007/s12275-014-3629-8

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