Enhanced Production of Carboxymethylcellulase by a Marine Bacterium, Bacillus velezensis A-68, by Using Rice Hulls in Pilot-scale Bioreactor under Optimized Conditions for Dissolved Oxygen

Wa Gao; Hye-Jin Kim; Chung-Han Chung; Jin-Woo Lee

doi:10.1007/s12275-014-4156-3

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Enhanced Production of Carboxymethylcellulase by a Marine Bacterium, Bacillus velezensis A-68, by Using Rice Hulls in Pilot-scale Bioreactor under Optimized Conditions for Dissolved Oxygen: Wa Gao ¹, Hye-Jin Kim ¹, Chung-Han Chung ², Jin-Woo Lee ¹; Journal of Microbiology 2014;52(9):755-761.
DOI: https://doi.org/10.1007/s12275-014-4156-3
Published online: July 30, 2014

¹Department of Biotechnology, Dong-A University, Busan 604-714, Republic of Korea , ²ReSEAT Programs, Korea Institute of Science and Technology Information, Seoul 130-741, Republic of Korea¹Department of Biotechnology, Dong-A University, Busan 604-714, Republic of Korea , ²ReSEAT Programs, Korea Institute of Science and Technology Information, Seoul 130-741, Republic of Korea

Corresponding author: Jin-Woo Lee , Tel: +82-51-200-7593,

Received: 12 March 2014 • Revised: 7 May 2014 • Accepted: 2 June 2014

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Abstract

The optimal conditions for the production of carboxymethylcellulase (CMCase) by Bacillus velezensis A-68 at a flask scale have been previously reported. In this study, the parameters involved in dissolved oxygen in 7 and 100 L bioreactors were optimized for the pilot-scale production of CMCase. The optimal agitation speed and aeration rate for cell growth of B. velezensis A-68 were 323 rpm and 1.46 vvm in a 7 L bioreactor, whereas those for the production of CMCase were 380 rpm and 0.54 vvm, respectively. The analysis of variance (ANOVA) implied that the highly significant factor for cell growth was the aeration rate, whereas that for the production of CMCase was the agitation speed. The optimal inner pressures for cell growth and the production of CMCase by B. velezensis A-68 in a 100 L bioreactor were 0.00 and 0.04 MPa, respectively. The maximal production of CMCase in a 100 L bioreactor under optimized conditions using rice hulls was 108.1 U/ml, which was 1.8 times higher than that at a flask scale under previously optimized conditions.

Keywords: Bacillus velezensis;carboxymethylcellulase;marine bacterium;response surface methodology;rice hulls

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Enhanced Production of Carboxymethylcellulase by a Marine Bacterium, Bacillus velezensis A-68, by Using Rice Hulls in Pilot-scale Bioreactor under Optimized Conditions for Dissolved Oxygen

J. Microbiol. 2014;52(9):755-761. Published online July 30, 2014

DOI: https://doi.org/10.1007/s12275-014-4156-3

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