Surface Display Expression of Bacillus licheniformis Lipase in Escherichia coli Using Lpp’OmpA Chimera

Jae-Hyung Jo; Chan-Wook Han; Seung-Hwan Kim; Hyuk-Jin Kwon; Hyune-Hwan Lee

doi:10.1007/s12275-014-4217-7

Articles

Page Path: HOME > J. Microbiol > Volume 52(10); 2014 > Article

Research Support, Non-U.S. Gov't
Surface Display Expression of Bacillus licheniformis Lipase in Escherichia coli Using Lpp’OmpA Chimera: Jae-Hyung Jo , Chan-Wook Han , Seung-Hwan Kim , Hyuk-Jin Kwon , Hyune-Hwan Lee; Journal of Microbiology 2014;52(10):856-862.
DOI: https://doi.org/10.1007/s12275-014-4217-7
Published online: August 27, 2014

Department of Bioscience and Biotechnology and Protein Research Center of GRRC, College of Natural Sciences, Hankuk University of Foreign Studies, Kyunggi-Do 449-791, Republic of KoreaDepartment of Bioscience and Biotechnology and Protein Research Center of GRRC, College of Natural Sciences, Hankuk University of Foreign Studies, Kyunggi-Do 449-791, Republic of Korea

Corresponding author: Hyune-Hwan Lee , Tel: +82-31-330-4280,

Received: 8 April 2014 • Revised: 11 July 2014 • Accepted: 15 July 2014

prev next

46 Views
0 Download
15 Crossref
17 Scopus

Full Article

Abstract

The lipase from Bacillus licheniformis ATCC14580 was displayed on the cell surface of Escherichia coli using Lpp’OmpA as the anchoring protein. The expressed Lpp’OmpA-lipase fusion protein has a molecular weight of approximately 35 kDa, which was confirmed by SDS-PAGE and western blot analysis. The Lpp’OmpA-lipase fusion protein was located on the cell surface, as determined by immunofluorescence confocal microscopy and flow cytometry. The enzyme activity of the surface-displayed lipase showed clear halo around the colony. The cell surface-displayed lipase showed the highest activity of 248.12 ± 9.42 U/g (lyophilized cell) at the optimal temperature of 37°C and pH 8.0. The enzyme exhibited the highest activity toward the substrate p-nitrophenyl caprylate (C8). These results suggest that E. coli, which displayed the lipase on its surface, could be used as a whole cell biocatalyst.

Keywords: surface display;Bacillus lichenformis;lipase;Lpp’OmpA;Escherichia coli

Figure & Data

References

Citations

Citations to this article as recorded by

Recent advances in bioinspired multienzyme engineering for food applications
Xianhan Chen, Yujin Chen, Dandan Tang, Mengyu Li, Yuting Lu, Yi Cao, Quanyu Zhao, Shuai Jiang, Wei Liu, Ling Jiang
Trends in Food Science & Technology.2025; 156: 104840. CrossRef
Surface Engineering of Escherichia coli to Display Its Phytase (AppA) and Functional Analysis of Enzyme Activities
Patricia L. A. Muñoz-Muñoz, Celina Terán-Ramírez, Rosa E. Mares-Alejandre, Ariana B. Márquez-González, Pablo A. Madero-Ayala, Samuel G. Meléndez-López, Marco A. Ramos-Ibarra
Current Issues in Molecular Biology.2024; 46(4): 3424. CrossRef
Characterization of a novel subfamily 1.4 lipase from Bacillus licheniformis IBRL-CHS2: Cloning and expression optimization
Ammar Khazaal Kadhim Almansoori, Nidyaletchmy Subba Reddy, Mustafa Abdulfattah, Sarah Solehah Ismail, Rashidah Abdul Rahim, Estibaliz Sansinenea
PLOS ONE.2024; 19(12): e0314556. CrossRef
Surface Display of Multiple Metal-Binding Domains in Deinococcus radiodurans Alleviates Cadmium and Lead Toxicity in Rice
Liangyan Wang, Yudong Wang, Shang Dai, Binqiang Wang
International Journal of Molecular Sciences.2024; 25(23): 12570. CrossRef
A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice
Jingjing Sun, Xuansheng Lin, Yige He, Baozhong Zhang, Nan Zhou, Jian-dong Huang
Frontiers in Immunology.2023;[Epub] CrossRef
Establishment of a soluble expression and rapid purification system for self-assembling protein nanoparticle and characterization of its physiochemical properties
Dan Wang, Linwei Duan, Min Wei, Baizhu Chen, Zhipeng Li, Qingyou Liu
Biochemical Engineering Journal.2022; 186: 108580. CrossRef
A Modular System for the Rapid Comparison of Different Membrane Anchors for Surface Display on Escherichia coli
Sabrina Gallus, Esther Mittmann, Kersten S. Rabe
ChemBioChem.2022;[Epub] CrossRef
Decorating the surface of Escherichia coli with bacterial lipoproteins: a comparative analysis of different display systems
Sonia Nicchi, Maria Giuliani, Fabiola Giusti, Laura Pancotto, Domenico Maione, Isabel Delany, Cesira L. Galeotti, Cecilia Brettoni
Microbial Cell Factories.2021;[Epub] CrossRef
Recombinant expression and surface display of a zearalenone lactonohydrolase from Trichoderma aggressivum in Escherichia coli
Shurong Chen, Li Pan, Siying Liu, Lijie Pan, Xuejie Li, Bin Wang
Protein Expression and Purification.2021; 187: 105933. CrossRef
Bacterial Cell Display as a Robust and Versatile Platform for Engineering Low‐Affinity Ligands and Enzymes
Eszter Csibra, Marleen Renders, Vitor B. Pinheiro
ChemBioChem.2020; 21(19): 2844. CrossRef
Surface Display of Complex Enzymes by in Situ SpyCatcher‐SpyTag Interaction
Sabrina Gallus, Theo Peschke, Malte Paulsen, Teresa Burgahn, Christof M. Niemeyer, Kersten S. Rabe
ChemBioChem.2020; 21(15): 2126. CrossRef
Shaking Rate during Production Affects the Activity of Escherichia coli Surface-Displayed Candida antarctica Lipase A
Chen-Fu Chung, Shih-Che Lin, Tzong-Yuan Juang, Yung-Chuan Liu
Catalysts.2020; 10(4): 382. CrossRef
Functional Display of an Amoebic Chitinase in Escherichia coli Expressing the Catalytic Domain of EhCHT1 on the Bacterial Cell Surface
Ricardo Torres-Bañaga, Rosa E. Mares-Alejandre, Celina Terán-Ramírez, Ana L. Estrada-González, Patricia L.A. Muñoz-Muñoz, Samuel G. Meléndez-López, Ignacio A. Rivero, Marco A. Ramos-Ibarra
Applied Biochemistry and Biotechnology.2020; 192(4): 1255. CrossRef
Heterologous expression of antigenic peptides in Bacillus subtilis biofilms
Cédric M. Vogt, Elisabeth M. Schraner, Claudio Aguilar, Catherine Eichwald
Microbial Cell Factories.2016;[Epub] CrossRef
Display of Fungi Xylanase on Escherichia coli Cell Surface and Use of the Enzyme in Xylan Biodegradation
Wei Qu, Yuanxia Xue, Qiang Ding
Current Microbiology.2015; 70(6): 779. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Surface Display Expression of Bacillus licheniformis Lipase in Escherichia coli Using Lpp’OmpA Chimera

J. Microbiol. 2014;52(10):856-862. Published online August 27, 2014

DOI: https://doi.org/10.1007/s12275-014-4217-7

Related articles