Synthetic Lethal Screen of NAA20, a Catalytic Subunit Gene of NatB N-Terminal Acetylase in Saccharomyces cerevisiae

Kang-Eun Lee; Jun-Young Ahn; Jeong-Mok Kim; Cheol-Sang Hwang

doi:10.1007/s12275-014-3694-z

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Synthetic Lethal Screen of NAA20, a Catalytic Subunit Gene of NatB N-Terminal Acetylase in Saccharomyces cerevisiae: Kang-Eun Lee , Jun-Young Ahn , Jeong-Mok Kim , Cheol-Sang Hwang; Journal of Microbiology 2014;52(10):842-848.
DOI: https://doi.org/10.1007/s12275-014-3694-z
Published online: August 27, 2014

Department of Life Sciences, Pohang University of Science and Technology, Gyeongbuk 790-784, Republic of KoreaDepartment of Life Sciences, Pohang University of Science and Technology, Gyeongbuk 790-784, Republic of Korea

Corresponding author: Cheol-Sang Hwang , Tel: +82-54-279-2353,

Received: 31 December 2013 • Revised: 30 June 2014 • Accepted: 24 July 2014

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Abstract

The Saccharomyces cerevisiae NatB N-terminal acetylase contains a catalytic subunit Naa20 and an auxiliary subunit Naa25. To elucidate the cellular functions of the NatB, we utilized the Synthetic Genetic Array to screen for genes that are essential for cell growth in the absence of NAA20. The genome-wide synthetic lethal screen of NAA20 identified genes encoding for serine/threonine protein kinase Vps15, 1,3-beta-glucanosyltransferase Gas5, and a catabolic repression regulator Mig3. The present study suggests that the catalytic activity of the NatB N-terminal aceytase is involved in vacuolar protein sorting and cell wall maintenance.

Keywords: N-terminal acetylation;synthetic lethal screen;NatB;Naa20;synthetic genetic array

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Synthetic Lethal Screen of NAA20, a Catalytic Subunit Gene of NatB N-Terminal Acetylase in Saccharomyces cerevisiae

J. Microbiol. 2014;52(10):842-848. Published online August 27, 2014

DOI: https://doi.org/10.1007/s12275-014-3694-z

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