Synthetic Lethal Screen of NAA20, a Catalytic Subunit Gene of NatB N-Terminal Acetylase in Saccharomyces cerevisiae

Kang-Eun Lee; Jun-Young Ahn; Jeong-Mok Kim; Cheol-Sang Hwang

doi:10.1007/s12275-014-3694-z

Articles

Page Path: HOME > J. Microbiol > Volume 52(10); 2014 > Article

Research Support, Non-U.S. Gov't
Synthetic Lethal Screen of NAA20, a Catalytic Subunit Gene of NatB N-Terminal Acetylase in Saccharomyces cerevisiae: Kang-Eun Lee , Jun-Young Ahn , Jeong-Mok Kim , Cheol-Sang Hwang; Journal of Microbiology 2014;52(10):842-848.
DOI: https://doi.org/10.1007/s12275-014-3694-z
Published online: August 27, 2014

Department of Life Sciences, Pohang University of Science and Technology, Gyeongbuk 790-784, Republic of KoreaDepartment of Life Sciences, Pohang University of Science and Technology, Gyeongbuk 790-784, Republic of Korea

Corresponding author: Cheol-Sang Hwang , Tel: +82-54-279-2353,

Received: 31 December 2013 • Revised: 30 June 2014 • Accepted: 24 July 2014

prev next

45 Views
0 Download
4 Crossref
4 Scopus

Full Article

Abstract

The Saccharomyces cerevisiae NatB N-terminal acetylase contains a catalytic subunit Naa20 and an auxiliary subunit Naa25. To elucidate the cellular functions of the NatB, we utilized the Synthetic Genetic Array to screen for genes that are essential for cell growth in the absence of NAA20. The genome-wide synthetic lethal screen of NAA20 identified genes encoding for serine/threonine protein kinase Vps15, 1,3-beta-glucanosyltransferase Gas5, and a catabolic repression regulator Mig3. The present study suggests that the catalytic activity of the NatB N-terminal aceytase is involved in vacuolar protein sorting and cell wall maintenance.

Keywords: N-terminal acetylation;synthetic lethal screen;NatB;Naa20;synthetic genetic array

Figure & Data

References

Citations

Citations to this article as recorded by

Fungal β-1, 3-glucanosyltransferases: A comprehensive review on classification, catalytic mechanism and functional role
Ting-Ting Wen, Zhuo-Yu Qian, Lei Sun, Feng-Jie Cui, Xin-Yi Zan, Li-Juan Meng, Wen-Jing Sun
International Journal of Biological Macromolecules.2025; 289: 138651. CrossRef
Structural basis of Naa20 activity towards a canonical NatB substrate
Dominik Layer, Jürgen Kopp, Miriam Fontanillo, Maja Köhn, Karine Lapouge, Irmgard Sinning
Communications Biology.2021;[Epub] CrossRef
Molecular basis for N-terminal alpha-synuclein acetylation by human NatB
Sunbin Deng, Buyan Pan, Leah Gottlieb, E James Petersson, Ronen Marmorstein
eLife.2020;[Epub] CrossRef
N-Terminal Acetylation-Targeted N-End Rule Proteolytic System: The Ac/N-End Rule Pathway
Kang-Eun Lee, Ji-Eun Heo, Jeong-Mok Kim, Cheol-Sang Hwang
Molecules and Cells.2016; 39(3): 169. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Synthetic Lethal Screen of NAA20, a Catalytic Subunit Gene of NatB N-Terminal Acetylase in Saccharomyces cerevisiae

J. Microbiol. 2014;52(10):842-848. Published online August 27, 2014

DOI: https://doi.org/10.1007/s12275-014-3694-z

Related articles