Transcriptional Regulation of fksA, a β-1,3-Glucan Synthase Gene, by the APSES Protein StuA during Aspergillus nidulans Development

Bum-Chan Park; Yun-Hee Park; Soohyun Yi; Yu Kyung Choi; Eun-Hye Kang; Hee-Moon Park

doi:10.1007/s12275-014-4517-y

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Transcriptional Regulation of fksA, a β-1,3-Glucan Synthase Gene, by the APSES Protein StuA during Aspergillus nidulans Development: Bum-Chan Park , Yun-Hee Park , Soohyun Yi , Yu Kyung Choi , Eun-Hye Kang , Hee-Moon Park; Journal of Microbiology 2014;52(11):940-947.
DOI: https://doi.org/10.1007/s12275-014-4517-y
Published online: October 31, 2014

Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of KoreaDepartment of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea

Corresponding author: Hee-Moon Park , Tel: +82-42-821-6417,

Received: 17 September 2014 • Revised: 6 October 2014 • Accepted: 6 October 2014

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Abstract

The temporal and spatial regulation of β-1,3-glucan synthesis plays an important role in morphogenesis during fungal growth and development. Northern blot analysis showed that the transcription of fksA, the gene encoding β-1,3-glucan synthase in Aspergillus nidulans, was cell-cycle-dependent and increased steadily over the duration of the vegetative period, but its overall expression during the asexual and sexual stages was fairly constant up until the time of transcription cessation. In an A. nidulans strain mutated in the eukaryotic bHLH-like APSES transcription factor stuA1, the transcriptional level of fksA, and consequently the content of alkali-insoluble cell wall β-glucan, significantly increased at the conidial chain formation and maturation stage. Electrophoretic mobility shift assays revealed that StuA was bound to StREs (StuA Response Elements) on the fksA promoter region. Promoter analysis with sGFP-fusion constructs also indicated the negative regulation of fksA expression by StuA, especially during asexual development. Taken together, these data suggest that StuA plays an important role in cell wall biogenesis during the development of A. nidulans, by controlling the transcription level of fksA.

Keywords: Aspergillus nidulans;β-1;3-glucan synthase gene;transcription;StuA;EMSA;sGFP-fusion

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Transcriptional Regulation of fksA, a β-1,3-Glucan Synthase Gene, by the APSES Protein StuA during Aspergillus nidulans Development

J. Microbiol. 2014;52(11):940-947. Published online October 31, 2014

DOI: https://doi.org/10.1007/s12275-014-4517-y

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