Proteomic and Functional Analyses of a Novel Porin-like Protein in Xanthomonas oryzae pv. oryzae

Hye-Jee Park; Sang-Won Lee; Sang-Wook Han

doi:10.1007/s12275-014-4442-0

Articles

Page Path: HOME > J. Microbiol > Volume 52(12); 2014 > Article

Research Support, Non-U.S. Gov't
Proteomic and Functional Analyses of a Novel Porin-like Protein in Xanthomonas oryzae pv. oryzae: Hye-Jee Park ¹, Sang-Won Lee ², Sang-Wook Han ¹; Journal of Microbiology 2014;52(12):1030-1035.
DOI: https://doi.org/10.1007/s12275-014-4442-0
Published online: November 29, 2014

¹Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Republic of Korea, ²Department of Plant Molecular Systems Biotechnology & Crop Biotech Institute & Graduate School of Biotechnology Kyung Hee University, Yongin 446-701, Republic of Korea¹Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Republic of Korea, ²Department of Plant Molecular Systems Biotechnology & Crop Biotech Institute & Graduate School of Biotechnology Kyung Hee University, Yongin 446-701, Republic of Korea

Corresponding author: Sang-Won Lee , Tel: +82-31-201-3473,
Sang-Wook Han , Tel: +82-31-201-3473,

Received: 31 July 2014 • Revised: 15 October 2014 • Accepted: 18 October 2014

prev next

43 Views
0 Download
13 Crossref
13 Scopus

Full Article

Abstract

Proteomic analysis is a useful technique for postulating and elucidating protein functions. In the present work, a shotgun proteomic analysis was used to identify functions of the PXO_03968 gene (previously known as the ax21) from Xanthomonas oryzae pv. oryzae (Xoo), a causal agent for bacterial blight disease in rice. Structural prediction performed on the protein sequence encoded by PXO_03968 reveals that it encodes a putative porin-like protein, possessing a β-barrel domain with 10 β-strands and a signal peptide at the Nterminus. We renamed the gene as an omp1X (outer membrane protein 1 in Xoo), generated its knock out mutant (XooΔomp1X), and compared the protein expression level in the mutant to that in the wild type. A total of 106 proteins displayed more than 1.5-fold difference in expression between the mutant and the wild type strains. COG analysis revealed that these proteins are involved in cell motility as well as signal transduction. In addition, phenotypic analysis demonstrated that motility and biofilm formation in XooΔomp1X are lower than the wild type. These results provide new insights into the functions of outer membrane proteins in Gram-negative bacteria.

Keywords: outer membrane protein;proteomics;motility;biofilm formation

Figure & Data

References

Citations

Citations to this article as recorded by

Stenotrophomonas maltophilia virulence: a current view
Vladimir Mikhailovich, Rustam Heydarov, Danila Zimenkov, Igor Chebotar
Frontiers in Microbiology.2024;[Epub] CrossRef
Molecular-genetic portrait of virulence of Stenotrophomonas maltophilia
Vladimir M. Mikhailovich, Rustam N. Geydarov, Julia A. Bocharova, Igor V. Chebotar
Journal of microbiology, epidemiology and immunobiology.2023; 100(5): 380. CrossRef
p‐Aminobenzoic acid inhibits the growth of soybean pathogen Xanthomonas axonopodis pv. glycines by altering outer membrane integrity
Yong‐Hui Jiang, Ting Liu, Xin‐Chi Shi, Daniela D Herrera‐Balandrano, Mei‐Ting Xu, Su‐Yan Wang, Pedro Laborda
Pest Management Science.2023; 79(10): 4083. CrossRef
Expression and function of clpS and clpA in Xanthomonas campestris pv. campestris
Hsueh-Hsia Lo, Hsiao-Ching Chang, Chao-Tsai Liao, Yi-Min Hsiao
Antonie van Leeuwenhoek.2022; 115(5): 589. CrossRef
Outer Membrane Vesicles: Biogenesis, Functions, and Issues
Rokas Juodeikis, Simon R. Carding
Microbiology and Molecular Biology Reviews.2022;[Epub] CrossRef
Recombinant Ax21 protein is a promising subunit vaccine candidate against Stenotrophomonas maltophilia in a murine infection model
Amal Tarek Sarhan, Mohammed Bahey-El-Din, Taha Ibrahim Zaghloul
Vaccine.2021; 39(32): 4471. CrossRef
Deciphering the functions of the outer membrane porin OprBXo involved in virulence, motility, exopolysaccharide production, biofilm formation and stress tolerance in Xanthomonas oryzae pv. oryzae
Nahee Bae, Hye‐Jee Park, Hanbi Park, Minyoung Kim, Sang‐Wook Han
Molecular Plant Pathology.2018; 19(12): 2527. CrossRef
The Ax21 protein influences virulence and biofilm formation in Stenotrophomonas maltophilia
Shi-qi An, Ji-liang Tang
Archives of Microbiology.2018; 200(1): 183. CrossRef
Lsp family proteins regulate antibiotic biosynthesis in Lysobacter enzymogenes OH11
Ruping Wang, Huiyong Xu, Yangyang Zhao, Juan Zhang, Gary Y Yuen, Guoliang Qian, Fengquan Liu
AMB Express.2017;[Epub] CrossRef
Systems and synthetic biology perspective of the versatile plant-pathogenic and polysaccharide-producing bacterium Xanthomonas campestris
Sarah Schatschneider, Jessica Schneider, Jochen Blom, Fabien Létisse, Karsten Niehaus, Alexander Goesmann, Frank-Jörg Vorhölter
Microbiology.2017; 163(8): 1117. CrossRef
Functional and Proteomic Analyses Reveal That ScpBXv Is Involved in Bacterial Growth, Virulence, and Biofilm Formation in Xanthomonas campestris pv. vesicatoria
Hye-Jee Park, Sang-Wook Han
The Plant Pathology Journal.2017; 33(6): 602. CrossRef
Deciphering the Role of Tyrosine Sulfation in Xanthomonas oryzae pv. oryzae Using Shotgun Proteomic Analysis
Hye-Jee Park, Chang-Jin Park, Nahee Bae, Sang-Wook Han
The Plant Pathology Journal.2016; 32(3): 266. CrossRef
The effect of imipenem and diffusible signaling factors on the secretion of outer membrane vesicles and associated Ax21 proteins in Stenotrophomonas maltophilia
Simon Devos, Laurence Van Oudenhove, Stephan Stremersch, Wouter Van Putte, Riet De Rycke, Gonzalez Van Driessche, Jolien Vitse, Koen Raemdonck, Bart Devreese
Frontiers in Microbiology.2015;[Epub] CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Proteomic and Functional Analyses of a Novel Porin-like Protein in Xanthomonas oryzae pv. oryzae

J. Microbiol. 2014;52(12):1030-1035. Published online November 29, 2014

DOI: https://doi.org/10.1007/s12275-014-4442-0

Related articles

Identification and Functional Analysis of Acyl‑Acyl Carrier Protein Δ9 Desaturase from Nannochloropsis oceanica