The Role of Wheat Germ Agglutinin in the Attachment of Pseudomonas sp. WS32 to Wheat Root

Jian Zhang; Liyuan Meng; Yuanyuan Cao; Huiping Chang; Zhongyou Ma; Leni Sun; Ming Zhang; Xinyun Tang

doi:10.1007/s12275-014-4089-x

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The Role of Wheat Germ Agglutinin in the Attachment of Pseudomonas sp. WS32 to Wheat Root: Jian Zhang ¹, Liyuan Meng ¹, Yuanyuan Cao ¹, Huiping Chang ², Zhongyou Ma ³, Leni Sun ¹, Ming Zhang ¹, Xinyun Tang ¹; Journal of Microbiology 2014;52(12):1020-1024.
DOI: https://doi.org/10.1007/s12275-014-4089-x
Published online: November 29, 2014

¹School of Life Sciences, Anhui Agricultural University, Hefei 230036, Anhui Province, P. R. China, ²Henan Institute of Education, Zhengzhou 450046, Henan Province, P. R. China, ³Department of Biology, Anhui Science and Technology University, Bengbu 233100, Anhui Province, P. R. China¹School of Life Sciences, Anhui Agricultural University, Hefei 230036, Anhui Province, P. R. China, ²Henan Institute of Education, Zhengzhou 450046, Henan Province, P. R. China, ³Department of Biology, Anhui Science and Technology University, Bengbu 233100, Anhui Province, P. R. China

Corresponding author: Yuanyuan Cao , Tel: +86-0551-65786319,
Xinyun Tang , Tel: +86-0551-65786319,

Received: 11 February 2014 • Revised: 31 August 2014 • Accepted: 22 October 2014

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Abstract

Wheat germ agglutinin (WGA), which is secreted on the surface of wheat root, has been defined as a protein that reversibly and non-enzymatically binds to specific carbohydrates. However, little attention has been paid to the function of WGA in the attachment of bacteria to their host plants. The aim of this study was to investigate the role of WGA in the attachment of Pseudomonas sp. WS32 to wheat roots. Wheat roots were initially treated with double-distilled water, WGA-H (WGA solution that was heated at 100°C for 15 min) and WGA, independently. Subsequently, the roots were coincubated with cell solutions (109 cells/ml). A dilution plate
method
using a solid nutrient medium was employed to determine the adsorption of WS32 to wheat roots. WGA was labeled with fluorescein isothiocyanate and detected using the fluorescent in situ hybridization (FISH) technique. The number of adsorptive WS32 cells on wheat roots was significantly increased when the wheat roots were pretreated with WGA, compared with the control treatment (p = 0.01). However, WGA-H failed to increase the amount of bacterial cells that attached to the wheat roots because of the loss of its physiological activity. The FISH assay also revealed that more cells adhered to WGA-treated wheat roots than to control or WGA-H-treated roots. The results indicated that WGA can mediate Pseudomonas strain WS32’s adherence to wheat seedling roots. The findings of this study provide a better understanding of the processes involved in plant-microbe interactions.

Keywords: wheat germ agglutinin;Pseudomonas sp.;attachment;wheat roots;fluorescent in situ hybridization

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Isolation and purification of wheat germ agglutinin and analysis of its properties
Han Wang
IOP Conference Series: Earth and Environmental Science.2017; 100: 012021. CrossRef

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The Role of Wheat Germ Agglutinin in the Attachment of Pseudomonas sp. WS32 to Wheat Root

J. Microbiol. 2014;52(12):1020-1024. Published online November 29, 2014

DOI: https://doi.org/10.1007/s12275-014-4089-x

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