Crystal structure of the bacterial type VI secretion system component TssL from Vibrio cholerae

Jeong Ho Chang; Yeon-Gil Kim

doi:10.1007/s12275-015-4539-0

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Crystal structure of the bacterial type VI secretion system component TssL from Vibrio cholerae: Jeong Ho Chang ¹, Yeon-Gil Kim ²; Journal of Microbiology 2015;53(1):32-37.
DOI: https://doi.org/10.1007/s12275-015-4539-0
Published online: December 4, 2014

¹Department of Biology, Teachers College, Kyungpook National University, Daegu 702-701, Republic of Korea, ²Beamline Science Division, Pohang Accelerator Laboratory, Pohang 790-784, Republic of Korea¹Department of Biology, Teachers College, Kyungpook National University, Daegu 702-701, Republic of Korea, ²Beamline Science Division, Pohang Accelerator Laboratory, Pohang 790-784, Republic of Korea

Corresponding author: Yeon-Gil Kim , Tel: +82-54-279-1544,

Received: 26 September 2014 • Revised: 27 October 2014 • Accepted: 27 October 2014

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Abstract

The type VI secretion system (T6SS), commonly found in Gram-negative bacteria, is responsible for exporting effector proteins. The T6SS has been reported to be cytotoxic to host cells. While the components and assembly of the T6SS complex have been largely assessed, structural data on T6SS components from virulent bacteria is remarkably insufficient. Here, we report the crystal structure of Vibrio cholerae TssL (VcTssL), a core component of T6SS. In spite of a relatively low sequence identity, the overall structure of VcTssL is largely similar to those from other bacterial homologs except for several differences found in local structural elements. A unique feature attributed to the C-terminal fragment of Vc- TssL is a crystallographic artifact. This incidental feature of VcTssL may provide insights into screening of molecular partners for the cytoplasmic domain of TssL. Additionally, our results may help in the design of molecular probes for a detailed understanding of the functional relationship between TssL and other T6SS components.

Keywords: type VI secretion system;TssL;Vibrio cholerae;X-ray crystallography

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Structural Characterization of TssL from Acinetobacter baumannii: a Key Component of the Type VI Secretion System
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Crystal structure of the bacterial type VI secretion system component TssL from Vibrio cholerae

J. Microbiol. 2015;53(1):32-37. Published online December 4, 2014

DOI: https://doi.org/10.1007/s12275-015-4539-0

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