Immunological charaterization of monoclonal antibodies used in rapid influenza diagnostic test for detection of the 2009 pandemic influenza A(H1N1)pdm09 infection

Hwajung Yi; Mi-Seon Lee; Joo-Yeon Lee; Hae Kyung Lee; Chun Kang

doi:10.1007/s12275-015-4642-2

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Immunological charaterization of monoclonal antibodies used in rapid influenza diagnostic test for detection of the 2009 pandemic influenza A(H1N1)pdm09 infection: Hwajung Yi ¹, Mi-Seon Lee ¹, Joo-Yeon Lee ¹, Hae Kyung Lee ¹, Chun Kang ^1,2; Journal of Microbiology 2015;53(2):166-175.
DOI: https://doi.org/10.1007/s12275-015-4642-2
Published online: January 28, 2015

¹Division of Influenza Virus, Center for Infectious Diseases, Korea National Institute of Health, Centers for Disease Control and Prevention, Cheongju 363-951, Republic of Korea, ²Division of AIDS, Center for Infectious Diseases, Korea National Institute of Health, Centers for Disease Control and Prevention, Cheongju 363-951, Republic of Korea¹Division of Influenza Virus, Center for Infectious Diseases, Korea National Institute of Health, Centers for Disease Control and Prevention, Cheongju 363-951, Republic of Korea, ²Division of AIDS, Center for Infectious Diseases, Korea National Institute of Health, Centers for Disease Control and Prevention, Cheongju 363-951, Republic of Korea

Corresponding author: Hwajung Yi , Tel: +82-43-719-8196,

Received: 5 November 2014 • Revised: 12 January 2015 • Accepted: 12 January 2015

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Abstract

Since the 2009 pandemic, monoclonal antibodies (mAbs) for rapid influenza diagnostic tests (RIDT) have been developed for specific diagnostics of pandemic viral infection. Most of the mAbs were poorly characterized because of urgency during the pandemic. Further characterization of the mAbs for RIDTs would be beneficial for understanding the immunological properties of the pandemic virus and utilizing the mAbs for other research purposes. In this study, it was confirmed that two mAbs (I38 and D383) in an RIDT for H1N1pdm09 diagnostics were able to detect H1N1pdm09 virus through enzyme-linked immunosorbent assay (ELISA) and immunofluorescence assay (IFA). Also, the two mAbs exhibited reactivity to hemagglutinins (HAs) of both the H1N1pdm09 and 1918 H1N1 viruses; therefore, the RIDT using the mAbs could detect HAs of H1N1pdm09 and also HAs of 1918 H1N1-like strains. In an extension to our previous study, the epitopes (Sa antigenic site and the interface area of F?and vestigial esterase subdomains on the HA1 domain of HA of H1N1pdm09) recognized by the mAbs were corroborated in depth by IFA with escape-mutants from the mAbs and mapping of the epitopes on the crystal structure of human H1N1 viral HAs. Collectively, these results imply that the mAbs for the RIDT may be suitable for use in studying the immunological properties of H1N1pdm09 viruses and that the Sa antigenic site and the interface area between F?and vestigial esterase subdomains on influenza viral HA recognized by the mAbs are immunologically conserved regions between H1N1pdm09 and 1918 H1N1.

Keywords: 2009 influenza pandemic;monoclonal antibody;RIDT;H1N1pdm09;1918 H1N1

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Immunological charaterization of monoclonal antibodies used in rapid influenza diagnostic test for detection of the 2009 pandemic influenza A(H1N1)pdm09 infection

J. Microbiol. 2015;53(2):166-175. Published online January 28, 2015

DOI: https://doi.org/10.1007/s12275-015-4642-2

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