Directed analysis of cyanobacterial membrane phosphoproteome using stained phosphoproteins and titanium-enriched phosphopeptides§

Dong-Gi Lee; Joseph Kwon; Chi-Yong Eom; Young-Moon Kang; Seong Woon Roh; Kyung-Bok Lee; Jong-Soon Choi

doi:10.1007/s12275-015-5021-8

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Research Support, Non-U.S. Gov't
Directed analysis of cyanobacterial membrane phosphoproteome using stained phosphoproteins and titanium-enriched phosphopeptides§: Dong-Gi Lee ¹, Joseph Kwon ¹, Chi-Yong Eom ², Young-Moon Kang ³, Seong Woon Roh ¹, Kyung-Bok Lee ¹, Jong-Soon Choi ^1,3; Journal of Microbiology 2015;53(4):279-287.
DOI: https://doi.org/10.1007/s12275-015-5021-8
Published online: April 8, 2015

¹Biological Disaster Analysis Group, Korea Basic Science Institute, Daejeon 305-806, Republic of Korea, ²Molecular Imaging Group, Korea Basic Science Institute, Seoul 120-140, Republic of Korea, ³Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, Republic of Korea¹Biological Disaster Analysis Group, Korea Basic Science Institute, Daejeon 305-806, Republic of Korea, ²Molecular Imaging Group, Korea Basic Science Institute, Seoul 120-140, Republic of Korea, ³Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, Republic of Korea

Corresponding author: Jong-Soon Choi , Tel: +82-42-865-3428,

Received: 13 January 2015 • Revised: 12 February 2015 • Accepted: 13 February 2015

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Abstract

Gel-free shotgun phosphoproteomics of unicellular cyanobacterium Synechocystis sp. PCC 6803 has not been reported up to now. The purpose of this study is to develop directed membrane phosphoproteomic method in Synechocystis sp. Total Synechocystis membrane proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and phosphoprotein-stained gel bands were selectively subjected to in-gel trypsin digestion. The phosphorylation sites of the resulting peptides were determined by assigning the neutral loss of [M-H3PO4] to Ser, Thr, and Tyr residues using nano-liquid chromatography 7 Tesla Fourier transform mass spectrometry. As an initial application, 111 proteins and 33 phosphoproteins were identified containing 11 integral membrane proteins. Identified four unknown phosphoproteins with transmembrane helices were suggested to be involved in membrane migration or transporters based on BLASTP search annotations. The overall distribution of hydrophobic amino acids in pTyr was lower in frequency than that of pSer or pThr. Positively charged amino acids were abundantly revealed in the surrounding amino acids centered on pTyr. A directed shotgun membrane phosphoproteomic strategy provided insight into understanding the fundamental regulatory processes underlying Ser, Thr, and Tyr phosphorylation in multi-layered membranous cyanobacteria.

Keywords: cyanobacterial membrane phosphoprotein;phospho-tagging;phospho-trapping;FT-MS

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Directed analysis of cyanobacterial membrane phosphoproteome using stained phosphoproteins and titanium-enriched phosphopeptides§

J. Microbiol. 2015;53(4):279-287. Published online April 8, 2015

DOI: https://doi.org/10.1007/s12275-015-5021-8

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