Anti-tumor effect of Cordyceps militaris in HCV-infected human hepatocarcinoma 7.5 cells

Seulki Lee; Hwan Hee Lee; Jisung Kim; Joohee Jung; Aree Moon; Choon-Sik Jeong; Hyojeung Kang; Hyosun Cho

doi:10.1007/s12275-015-5198-x

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Research Support, Non-U.S. Gov't
Anti-tumor effect of Cordyceps militaris in HCV-infected human hepatocarcinoma 7.5 cells: Seulki Lee ^1,2, Hwan Hee Lee ^1,2, Jisung Kim ¹, Joohee Jung ^1,2, Aree Moon ^1,2, Choon-Sik Jeong ^1,2, Hyojeung Kang ³, Hyosun Cho ^1,2; Journal of Microbiology 2015;53(7):468-474.
DOI: https://doi.org/10.1007/s12275-015-5198-x
Published online: June 27, 2015

¹College of Pharmacy, Duksung Women’s University, Seoul 132-714, Republic of Korea, ²Innovative Drug Center, Duksung Women’s University, Seoul 132-714, Republic of Korea, ³College of Pharmacy, Research Institute of Pharmaceutical Sciences and Institute for Microorganisms, Kyungpook National University, Daegu 702-701, Republic of Korea¹College of Pharmacy, Duksung Women’s University, Seoul 132-714, Republic of Korea, ²Innovative Drug Center, Duksung Women’s University, Seoul 132-714, Republic of Korea, ³College of Pharmacy, Research Institute of Pharmaceutical Sciences and Institute for Microorganisms, Kyungpook National University, Daegu 702-701, Republic of Korea

Corresponding author: Hyojeung Kang , Tel: +82-2-901-8678,
Hyosun Cho , Tel: +82-2-901-8678,

Received: 15 April 2015 • Revised: 21 May 2015 • Accepted: 26 May 2015

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Abstract

Cordyceps extract has been reported to have various pharmacological activities including an anti-cancer effect. We investigated the inhibitory effect of Cordyceps militaris on hepatitis C virus-infected human hepatocarcinoma 7.5 cells (J6/JFH1-huh 7.5 cells). The huh7.5 cells with or without HCV infection were treated with various concentrations of ethanol extract of Cordyceps militaris (CME) for 48 h and the cytotoxicity was measured by CCK-8 assay. Both J6/JFH1- huh7.5 cells and huh7.5 cells were highly susceptible to CME. To examine the molecular mechanisms of the inhibitory effect on huh7.5 cells, the effect of CME on cell apoptosis was measured using flow cytometry and the expressions of p53, Bim, Bax, PARP, (cleaved) caspase-9, and (cleaved) caspase- 3 in huh 7.5 cells were detected by western blot assays. CME significantly increased early apoptosis and up-regulated the expression of Bim, Bax, cleaved PARP, cleaved caspase 9 and cleaved caspase-3. We also found the decrease of HCV Core or NS3 protein by CME in HCV-infected huh 7.5 cells.

Keywords: Cordyceps militaris;human hepatocarcinoma 7.5 cells;HCV;apoptosis;Bax;PARP

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Anti-tumor effect of Cordyceps militaris in HCV-infected human hepatocarcinoma 7.5 cells

J. Microbiol. 2015;53(7):468-474. Published online June 27, 2015

DOI: https://doi.org/10.1007/s12275-015-5198-x

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