Characterization of the rapamycin-inducible EBV LMP1 activation system

Sang Yong Kim; Jung-Eun Kim; Jiyeon Won; Yoon-Jae Song

doi:10.1007/s12275-015-5455-z

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Characterization of the rapamycin-inducible EBV LMP1 activation system: Sang Yong Kim , Jung-Eun Kim , Jiyeon Won , Yoon-Jae Song; Journal of Microbiology 2015;53(10):732-738.
DOI: https://doi.org/10.1007/s12275-015-5455-z
Published online: October 2, 2015

Department of Life Science, Gachon University, Seongnam 13120, Republic of KoreaDepartment of Life Science, Gachon University, Seongnam 13120, Republic of Korea

Corresponding author: Yoon-Jae Song , Tel: +82-31-750-8731,

Received: 8 September 2015 • Revised: 14 September 2015 • Accepted: 15 September 2015

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Abstract

Epstein-Barr virus (EBV) latent infection membrane protein 1 (LMP1) is required for EBV-mediated B lymphocyte transformation into proliferating lymphoblastoid cell lines (LCL). LMP1 oligomerizes spontaneously in membrane lipid rafts via its transmembrane domain and constitutively activates signal transduction pathways, including NF-κB, p38 Mitogen-Activated Protein Kinase (MAPK), and c-Jun N-terminal Kinase (JNK). Since LMP1 mimics the tumor necrosis factor receptor (TNFR), CD40, it may be effectively utilized to study the effects of constitutive activation of signal transduction pathways on cellular physiology. On the other hand, LMP1 presents a disadvantage in terms of determining the sequential events and factors involved in signaling pathways. A CD40-LMP1 chimeric molecule has been generated to overcome this limitation but does not represent the authentic and physiological nature of LMP1. In the current study, a ligand-dependent activation system for LMP1 using rapamycin-inducible dimerization was generated to delineate the LMP1 signaling pathway.

Keywords: Epstein-Barr virus;latent membrane protein 1;NF-κB

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Characterization of the rapamycin-inducible EBV LMP1 activation system

J. Microbiol. 2015;53(10):732-738. Published online October 2, 2015

DOI: https://doi.org/10.1007/s12275-015-5455-z

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