Abstract

Strain JSH 5-14T, a Gram-negative, non-motile, and curved rod-shaped bacterium, was isolated from a dust sample collected at Nonsan, Chungnam province, South Korea, and was characterized to determine its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence of strain JSH 5-14T revealed that it belongs to the genus Spirosoma, family Cytophagaceae, class Cytophagia. The highest degree of sequence similarities of strain JSH 5-14T were found with Spirosoma liguale DSM 74T (97.8%) and Spirosoma endophyticum EX 36T (96.2%). The predominant fatty acids were summed feature 3 (composed of C16:1 ω7c/C16:1 ω6c) and C16:1ω5c. The major polar lipid was phosphatidylethanolamine, and the predominant respiratory quinone was MK-7. Based on the phylogenetic, chemotaxonomic, and phenotypic data, we propose the strain JSH 5-14T (=KCTC 42550T =JCM 30688T =KEMB 9004-165T) should be classified as a type strain of a novel species, for which the name Spirosoma pulveris sp. nov., is proposed.

• Keywords: Cytophagaceae; Cytophagia; Spirosoma; taxonomy