Novel nuclear targeting coiled-coil protein of Helicobacter pylori showing Ca2+-independent, Mg2+-dependent DNase I activity

Young Chul Kwon; Sinil Kim; Yong Seok Lee; Je Chul Lee; Myung-Je Cho; Woo-Kon Lee; Hyung-Lyun Kang; Jae-Young Song; Seung Chul Baik; Hyeon Su Ro

doi:10.1007/s12275-016-5631-9

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Novel nuclear targeting coiled-coil protein of Helicobacter pylori showing Ca2+-independent, Mg2+-dependent DNase I activity: Young Chul Kwon ¹, Sinil Kim ², Yong Seok Lee ³, Je Chul Lee ⁴, Myung-Je Cho ¹, Woo-Kon Lee ¹, Hyung-Lyun Kang ¹, Jae-Young Song ¹, Seung Chul Baik ¹, Hyeon Su Ro ²; Journal of Microbiology 2016;54(5):387-395.
DOI: https://doi.org/10.1007/s12275-016-5631-9
Published online: April 20, 2016

¹Department of Microbiology, Gyeongsang National University School of Medicine, Jinju 52727, Republic of Korea, ²Division of Life Science and Research Institute of Life Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea, ³Department of Life Science and Biotechnology, Soonchunhyang University, Asan 31538, Republic of Korea, ⁴Department of Microbiology, Kyungpook National University School of Medicine, Daegu 41944, Republic of Korea¹Department of Microbiology, Gyeongsang National University School of Medicine, Jinju 52727, Republic of Korea, ²Division of Life Science and Research Institute of Life Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea, ³Department of Life Science and Biotechnology, Soonchunhyang University, Asan 31538, Republic of Korea, ⁴Department of Microbiology, Kyungpook National University School of Medicine, Daegu 41944, Republic of Korea

Corresponding author: Hyeon Su Ro , Tel: -,

Received: 22 December 2015 • Revised: 30 March 2016 • Accepted: 30 March 2016

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Abstract

HP0059, an uncharacterized gene of Helicobacter pylori, encodes a 284-aa-long protein containing a nuclear localization sequence (NLS) and multiple leucine-rich heptad repeats. Effects of HP0059 proteins in human stomach cells were assessed by incubation of recombinant HP0059 proteins with the AGS human gastric carcinoma cell line. Wild-type HP0059 proteins showed cytotoxicity in AGS cells in a concentrationdependent manner, whereas NLS mutant protein showed no effect, suggesting that the cytotoxicity is attributed to host nuclear localization. AGS cells transfected with pEGFP-HP0059 plasmid showed strong GFP signal merged to the chromosomal DNA region. The chromosome was fragmented into multiple distinct dots merged with the GFP signal after 12 h of incubation. The chromosome fragmentation was further explored by incubation of AGS chromosomal DNA with recombinant HP0059 proteins, which leaded to complete degradation of the chromosomal DNA. HP0059 protein also degraded circular plasmid DNA without consensus, being an indication of DNase I activity. The DNase was activated by MgCl2, but not by CaCl2. The activity was completely blocked by EDTA. The optimal pH and temperature for DNase activity were 7.0–8.0 and 55°C, respectively. These results indicate that HP0059 possesses a novel DNase I activity along with a role in the genomic instability of human gastric cells, which may result in the transformation of gastric cells.

Keywords: coiled-coil;DNase I;Helicobacter pylori;HP0059

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Novel nuclear targeting coiled-coil protein of Helicobacter pylori showing Ca2+-independent, Mg2+-dependent DNase I activity

J. Microbiol. 2016;54(5):387-395. Published online April 20, 2016

DOI: https://doi.org/10.1007/s12275-016-5631-9

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