Diversity of indigenous endophytic bacteria associated with the roots of Chinese cabbage (Brassica campestris L.) cultivars and their antagonism towards pathogens

Md. Azizul Haque; Han Dae Yun; Kye Man Cho

doi:10.1007/s12275-016-5641-7

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Diversity of indigenous endophytic bacteria associated with the roots of Chinese cabbage (Brassica campestris L.) cultivars and their antagonism towards pathogens: Md. Azizul Haque ^1,2, Han Dae Yun ³, Kye Man Cho ¹; Journal of Microbiology 2016;54(5):353-363.
DOI: https://doi.org/10.1007/s12275-016-5641-7
Published online: April 20, 2016

¹Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 660-758, Republic of Korea, ²Department of Biochemistry & Molecular Biology, Hajee Mohammad Danesh Science & Technology University, Dinajpur 5200, Bangladesh, ³Research Institute of Life Sciences, Gyeongsang National University, Jinju 660-701, Republic of Korea¹Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 660-758, Republic of Korea, ²Department of Biochemistry & Molecular Biology, Hajee Mohammad Danesh Science & Technology University, Dinajpur 5200, Bangladesh, ³Research Institute of Life Sciences, Gyeongsang National University, Jinju 660-701, Republic of Korea

Corresponding author: Kye Man Cho , Tel: +82-55-751-3272,

Received: 28 December 2015 • Revised: 4 April 2016 • Accepted: 6 April 2016

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Abstract

The study aimed to reveal the diversity of endophytic bacteria in the roots of Chinese cabbage (CC) cultivated in two areas in Korea, namely, Seosang-gun (SS) and Haenam-gun (HN), and also in a transgenic plant (TP) from the laboratory. A total of 653 colonies were isolated from the interior of CC roots, comprising 118, 302, and 233 isolates from SS, HN, and TP samples, respectively. Based on 16S rRNA gene sequence analysis, the isolates belonged to four major phylogenetic groups: high-G+C Gram-positive bacteria (HGC-GPB), low-G+C Gram-positive bacteria (LGC-GPB), Proteobacteria, and Bacteriodetes. The most dominant groups in the roots of the SS, HN, and TP cultivars were LGC-GPB (48.3%), Proteobacteria (50.2%), and HGC-GPB (38.2%), respectively. Importantly, most of the isolates that produced cell-walldegrading enzymes belonged to the genus Bacillus. Bacillus sp. (HNR03, TPR06), Bacillus pumilus (SSR07, HNR11, TPR07), and Bacillus subtilis (TPR03) showed high antagonism against the tested food-borne pathogenic bacteria. In addition, Bacillus sp. (HNR03, TPR06), Bacillus pumilus (SSR07, HNR11, HNR17, TPR11), Microbacterium oxidans (SSR09, TPR04), Bacillus cereus HNR10, Pseudomonas sp. HNR13, and Bacillus subtilis (TPR02, TPR03) showed strong antagonistic activity against the fungi Phythium ultimum, Phytophthora capsici, Fusarium oxysporum, and Rhizoctonia solani. The endophytes isolated from the TP cultivar showed the strongest antagonistic reactions against pathogens. This study is the first report on endophytic bacteria from Chinese cabbage roots.

Keywords: Chinese cabbage root;endophytic bacteria;phylogene;antimicrobial activity

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Diversity of indigenous endophytic bacteria associated with the roots of Chinese cabbage (Brassica campestris L.) cultivars and their antagonism towards pathogens

J. Microbiol. 2016;54(5):353-363. Published online April 20, 2016

DOI: https://doi.org/10.1007/s12275-016-5641-7

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