PprM is necessary for up-regulation of katE1, encoding the major catalase of Deinococcus radiodurans, under unstressed culture conditions

Sun-Wook Jeong; Ho Seong Seo; Min-Kyu Kim; Jong-Il Choi; Heon-Man Lim; Sangyong Lim

doi:10.1007/s12275-016-6175-8

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PprM is necessary for up-regulation of katE1, encoding the major catalase of Deinococcus radiodurans, under unstressed culture conditions: Sun-Wook Jeong ^1,2, Ho Seong Seo ¹, Min-Kyu Kim ¹, Jong-Il Choi ³, Heon-Man Lim ², Sangyong Lim ¹; Journal of Microbiology 2016;54(6):426-431.
DOI: https://doi.org/10.1007/s12275-016-6175-8
Published online: May 27, 2016

¹Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea, ²Department of Biological Sciences, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea, ³Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 61186, Republic of Korea¹Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea, ²Department of Biological Sciences, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea, ³Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 61186, Republic of Korea

Corresponding author: Sangyong Lim , Tel: -,

Received: 15 April 2016 • Revised: 19 May 2016 • Accepted: 19 May 2016

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Abstract

Deinococcus radiodurans is a poly-extremophilic organism, capable of tolerating a wide variety of different stresses, such as gamma/ultraviolet radiation, desiccation, and oxidative stress. PprM, a cold shock protein homolog, is involved in the radiation resistance of D. radiodurans, but its role in the oxidative stress response has not been investigated. In this study, we investigated the effect of pprM mutation on catalase gene expression. pprM disruption decreased the mRNA and protein levels of KatE1, which is the major catalase in D. radiodurans, under normal culture conditions. A pprM mutant strain (pprMMT) exhibited decreased catalase activity, and its resistance to hydrogen peroxide (H2O2) decreased accordingly compared with that of the wild-type strain. We confirmed that RecG helicase negatively regulates katE1 under normal culture conditions. Among katE1 transcriptional regulators, the positive regulator drRRA was not altered in pprM-, while the negative regulators perR, dtxR, and recG were activated more than 2.5-fold in pprMMT. These findings suggest that PprM is necessary for KatE1 production under normal culture conditions by down-regulation of katE1 negative regulators.

Keywords: PprM;cold-shock protein;ROS;katE1;catalase activity

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PprM is necessary for up-regulation of katE1, encoding the major catalase of Deinococcus radiodurans, under unstressed culture conditions

J. Microbiol. 2016;54(6):426-431. Published online May 27, 2016

DOI: https://doi.org/10.1007/s12275-016-6175-8

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