MINIREVIEW] On the study of microbial transcriptomes using second- and third-generation sequencing technologies

Sang Chul Choi

doi:10.1007/s12275-016-6233-2

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MINIREVIEW] On the study of microbial transcriptomes using second- and third-generation sequencing technologies: Sang Chul Choi; Journal of Microbiology 2016;54(8):527-536.
DOI: https://doi.org/10.1007/s12275-016-6233-2
Published online: August 2, 2016

Department of Biology, Sungshin Women's University, Seoul 01133, Republic of KoreaDepartment of Biology, Sungshin Women's University, Seoul 01133, Republic of Korea

Corresponding author: Sang Chul Choi , Tel: +82-2-920-2751,

Received: 20 May 2016 • Revised: 16 June 2016 • Accepted: 16 June 2016

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Abstract

Second-generation sequencing technologies transformed the study of microbial transcriptomes. They helped reveal the transcription start sites and antisense transcripts of microbial species, improving the microbial genome annotation. Quantification of genome-wide gene expression levels allowed for functional studies of microbial research. Ever-evolving sequencing technologies are reshaping approaches to studying microbial transcriptomes. Recently, Oxford Nanopore Technologies delivered a sequencing platform called MinION, a third-generation sequencing technology, to the research community. We expect it to be the next sequencing technology that enables breakthroughs in life science fields. The studies of microbial transcriptomes will be no exception. In this paper, we review microbial transcriptomics studies using second- generation sequencing technology. We also discuss the prospect of microbial transcriptomics studies with thirdgeneration sequencing.

Keywords: library preparation;bioinformatics;quality control;RNA-seq;differential expression;functional enrichment;Oxford Nanopore MinION

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MINIREVIEW] On the study of microbial transcriptomes using second- and third-generation sequencing technologies

J. Microbiol. 2016;54(8):527-536. Published online August 2, 2016

DOI: https://doi.org/10.1007/s12275-016-6233-2

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