Identification of essential genes of Pseudomonas aeruginosa for its growth in airway mucus

Mohammed Abd Alrahman; Sang Sun Yoon

doi:10.1007/s12275-017-6515-3

Articles

Page Path: HOME > J. Microbiol > Volume 55(1); 2017 > Article

Journal Article
Identification of essential genes of Pseudomonas aeruginosa for its growth in airway mucus: Mohammed Abd Alrahman ¹, Sang Sun Yoon ²; Journal of Microbiology 2017;55(1):68-74.
DOI: https://doi.org/10.1007/s12275-017-6515-3
Published online: December 30, 2016

¹Department of Microbiology and Immunology, Brain Korea 21 PLUS Project for Medical Science Yonsei University College of Medicine, Seoul 03722, Republic of Korea, ²Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul 03722, Republic of Korea¹Department of Microbiology and Immunology, Brain Korea 21 PLUS Project for Medical Science Yonsei University College of Medicine, Seoul 03722, Republic of Korea, ²Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul 03722, Republic of Korea

Corresponding author: Sang Sun Yoon , Tel: -,

Received: 10 October 2016 • Revised: 21 October 2016 • Accepted: 21 October 2016

prev next

47 Views
0 Download
8 Crossref
9 Scopus

Full Article

Abstract

Pseudomonas aeruginosa has been identified as an important causative agent of airway infection, mainly in cystic fibrosis. This disease is characterized by defective mucociliary clearance induced in part by mucus hyper-production. Mucin is a major component of airway mucus and is heavily O-glycosylated, with a protein backbone. Airway infection is known to be established with bacterial adhesion to mucin. However, the genes involved in mucin degradation or utilization remain elusive. In this study, we sought to provide a genetic basis of P. aeruginosa airway growth by identifying those genes. First, using RNASeq analyses, we compared genome-wide expression profiles of PAO1, a prototype P. aeruginosa laboratory strain, grown in M9-mucin (M9M) and M9-glucose (M9G) media. Additionally, a PAO1 transposon (Tn) insertion mutants library was screened for mutants defective in growth in M9M medium. One mutant with a Tn insertion in the xcpU gene (PA3100) was determined to exhibit faulty growth in M9M medium. This gene contributes to the type II secretion system, suggesting that P. aeruginosa uses this secretion system to produce a number of proteins to break down and assimilate the mucin molecule. Furthermore, we screened the PAO1 genome for genes with protease activity. Of 13 mutants, one with mutation in PA3247 gene exhibited defective growth in M9M, suggesting that the PA3247-encoded protease plays a role in mucin utilization. Further mechanistic dissection of this particular process will reveal new drug targets, the inhibition of which could control recalcitrant P. aeruginosa infections.

Keywords: Pseudomonas aeruginosa;airway infection;mucin;type II secretion system;protease

Figure & Data

References

Citations

Citations to this article as recorded by

Citrobacter rodentium possesses a functional type II secretion system necessary for successful host infection
Z Krekhno, SE Woodward, A Serapio-Palacios, J Peña-Díaz, KM Moon, LJ Foster, BB Finlay
Gut Microbes.2024;[Epub] CrossRef
Cross-talk between cancer and Pseudomonas aeruginosa mediates tumor suppression
Juliana K. Choi, Samer A. Naffouje, Masahide Goto, Jing Wang, Konstantin Christov, David J. Rademacher, Albert Green, Arlene A. Stecenko, Ananda M. Chakrabarty, Tapas K. Das Gupta, Tohru Yamada
Communications Biology.2023;[Epub] CrossRef
Pseudomonas aeruginosa type IV pili actively induce mucus contraction to form biofilms in tissue-engineered human airways
Tamara Rossy, Tania Distler, Lucas A. Meirelles, Joern Pezoldt, Jaemin Kim, Lorenzo Talà, Nikolaos Bouklas, Bart Deplancke, Alexandre Persat, Victor Sourjik
PLOS Biology.2023; 21(8): e3002209. CrossRef
Impact of diet and the bacterial microbiome on the mucous barrier and immune disorders
Charlotte A. Alemao, Kurtis F. Budden, Henry M. Gomez, Saima F. Rehman, Jacqueline E. Marshall, Shakti D. Shukla, Chantal Donovan, Samuel C. Forster, Ian A. Yang, Simon Keely, Elizabeth R. Mann, Emad M. El Omar, Gabrielle T. Belz, Philip M. Hansbro
Allergy.2021; 76(3): 714. CrossRef
The Bactericidal Tandem Drug, AB569: How to Eradicate Antibiotic-Resistant Biofilm Pseudomonas aeruginosa in Multiple Disease Settings Including Cystic Fibrosis, Burns/Wounds and Urinary Tract Infections
Daniel J. Hassett, Rhett A. Kovall, Michael J. Schurr, Nalinikanth Kotagiri, Harshita Kumari, Latha Satish
Frontiers in Microbiology.2021;[Epub] CrossRef
Structural and functional analysis of the carotenoid biosynthesis genes of aPseudomonasstrain isolated from the excrement of Autumn Darter
Yuki Fukaya, Miho Takemura, Takashi Koyanagi, Takashi Maoka, Kazutoshi Shindo, Norihiko Misawa
Bioscience, Biotechnology, and Biochemistry.2018; 82(6): 1043. CrossRef
Evolutionary conservation of the antimicrobial function of mucus: a first defence against infection
Cassie R Bakshani, Ana L Morales-Garcia, Mike Althaus, Matthew D Wilcox, Jeffrey P Pearson, John C Bythell, J Grant Burgess
npj Biofilms and Microbiomes.2018;[Epub] CrossRef
Expanding Role of Type II Secretion in Bacterial Pathogenesis and Beyond
Nicholas P. Cianciotto, Richard C. White, Anthony T. Maurelli
Infection and Immunity.2017;[Epub] CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Identification of essential genes of Pseudomonas aeruginosa for its growth in airway mucus

J. Microbiol. 2017;55(1):68-74. Published online December 30, 2016

DOI: https://doi.org/10.1007/s12275-017-6515-3

Related articles